#vanEunen1

FIX: ACE ADP AMP ATP ETOH F26BP GLCo GLY T6P TREH

#reactions
v_1:
 {1.0}GLCo = {1.0}GLCi
  (VMAXGLT*(GLCo/KMGLTGLCo - GLCi/(KMGLTGLCo*KEQGLT)))/(1 + GLCo/KMGLTGLCo + GLCi/KMGLTGLCi + 0.91*(GLCo*GLCi)/(KMGLTGLCo*KMGLTGLCi))
v_10:
 {1.0}BPG + {1.0}ADP = {1.0}P3G + {1.0}ATP
  (VMAXPGK*((KEQPGK*BPG*ADP)/(KMPGKP3G*KMPGKATP) - (P3G*ATP)/(KMPGKP3G*KMPGKATP)))/((1 +BPG/KMPGKBPG + P3G/KMPGKP3G)*(1 + ATP/KMPGKATP + ADP/KMPGKADP))
v_11:
 {1.0}P3G = {1.0}P2G
  (VMAXPGM*(P3G/KMPGMP3G - P2G/(KMPGMP3G*KEQPGM)))/(1 + P3G/KMPGMP3G + P2G/KMPGMP2G)
v_12:
 {1.0}P2G = {1.0}PEP
  (VMAXENO*(P2G/KMENOP2G - PEP/(KMENOP2G*KEQENO)))/(1 + P2G/KMENOP2G + PEP/KMENOPEP)
v_13:
 {1.0}PEP + {1.0}ADP = {1.0}PYR + {1.0}ATP
  (VMAXPYK*PEP/KMPYKPEP*(1 + PEP/KMPYKPEP)**(n10 - 1))/(L10*((ATP/KMPYKATP + 1)/(F16P/KMPYKF16P +1))**n10 + (1 + PEP/KMPYKPEP)**n10)*ADP/(ADP + KMPYKADP)
v_14:
 {1.0}PYR = {1.0}ACALD
  (VMAXPDC*PYR**NHPDC)/(KMPDCPYR**NHPDC*(1 + PYR**NHPDC/KMPDCPYR**NHPDC))
v_15:
 {2.0}PYR = {3.0}NADH
  KSUC
v_16:
 {1.0}ACALD + {1.0}NADH = {1.0}ETOH
  -(VMAXADH*(((NADt - NADH)*ETOH)/(KIADHNAD*KMADHETOH) - (NADH*ACALD)/(KIADHNAD*KMADHETOH*KEQADH)))/((1 + (NADt - NADH)/KIADHNAD + (KMADHNAD*ETOH)/(KIADHNAD*KMADHETOH) + (KMADHNADH*ACALD)/(KIADHNADH*KMADHACALD) + NADH/KIADHNADH + ((NADt - NADH)* ETOH)/(KIADHNAD*KMADHETOH) + (KMADHNADH *(NADt - NADH)*ACALD)/(KIADHNAD*KIADHNADH*KMADHACALD)  + (KMADHNAD*ETOH*NADH)/(KIADHNAD*KMADHETOH*KIADHNADH) + (NADH*ACALD)/(KIADHNADH*KMADHACALD) + ((NADt - NADH)* ETOH*ACALD)/(KIADHNAD*KMADHETOH*KIADHACALD) + (ETOH*NADH*ACALD)/(KIADHETOH*KIADHNADH*KMADHACALD)))
v_17:
 {1.0}ACALD = {1.0}NADH + {1.0}ACE
  KACE*ACALD
v_2:
 {1.0}GLCi + {1.0}ATP = {1.0}G6P + {1.0}ADP
  (VMAXHK*(GLCi/KMHKGLCi*ATP/KMHKATP - (G6P*ADP)/(KMHKGLCi*KMHKATP*KEQHK) ))/((1 + GLCi/KMHKGLCi + G6P/KMHKG6P + T6P/KIHKT6P)*(1 + ATP/KMHKATP + ADP/KMHKADP))
v_3:
 {1.0}TREH = {2.0}GLCi
  KTRE1
v_4:
 {1.0}G6P = {1.0}F6P
  (VMAXPGI*(G6P/KMPGIG6P - F6P/(KMPGIG6P*KEQPGI)))/(1 + G6P/KMPGIG6P + F6P/KMPGIF6P)
v_5:
 {2.0}G6P + {1.0}ATP = {1.0}TREH + {1.0}ADP
  KTRE2
v_6:
 {1.0}F6P + {1.0}ATP = {1.0}F16P + {1.0}ADP
  (VMAXPFK*gR*F6P*ATP*(1 + F6P/KMPFKF6P*ATP/KMPFKATP + (gR*F6P*ATP)/(KMPFKF6P*KMPFKATP)))/(KMPFKF6P*KMPFKATP*((1 + F6P/KMPFKF6P*ATP/KMPFKATP + (gR*F6P*ATP)/(KMPFKF6P*KMPFKATP))**2 + ((L0*(1 + (CiPFKATP*ATP)/KiPFKATP)**2*(1 + (CPFKAMP*AMP)/KPFKAMP)**2*(1 + (CPFKF26BP*F26BP)/KPFKF26BP + (CPFKF16BP*F16P)/KPFKF16BP)**2)/((1 + ATP/KiPFKATP)**2*(1 + AMP/KPFKAMP)**2*(1 + F26BP/KPFKF26BP + F16P/KPFKF16BP)**2))*(1 + (CPFKATP*ATP)/KMPFKATP)**2))
v_7:
 {1.0}F16P = {2.0}TRIO
  (VMAXALD*(F16P/KMALDF16P - (KEQTPI/(1 + KEQTPI)*TRIO*1/(1 + KEQTPI)*TRIO)/(KMALDF16P*KEQALD)))/(1 + F16P/KMALDF16P + (KEQTPI/(1 + KEQTPI)*TRIO)/KMALDGAP + (1/(1 + KEQTPI)*TRIO)/KMALDDHAP + (KEQTPI/(1 + KEQTPI)*TRIO*1/(1 + KEQTPI)*TRIO)/(KMALDGAP*KMALDDHAP) + (F16P*KEQTPI/(1 + KEQTPI)*TRIO)/( KMALDGAPi*KMALDF16P))
v_8:
 {1.0}NADH + {1.0}TRIO = {1.0}GLY
  KGLY
v_9:
 {1.0}TRIO = {1.0}BPG + {1.0}NADH
  (CGAPDH*((VMAXGAPDHf*KEQTPI/(1 + KEQTPI)*TRIO*(NADt - NADH))/(KMGAPDHGAP*KMGAPDHNAD) -(VMAXGAPDHr*BPG*NADH)/(KMGAPDHBPG*KMGAPDHNADH)))/((1 + (KEQTPI/(1 + KEQTPI)*TRIO)/KMGAPDHGAP + BPG/KMGAPDHBPG)*(1 + (NADt - NADH)/KMGAPDHNAD +NADH/KMGAPDHNADH))


#parameters
CGAPDH = 1.0
CPFKAMP = 0.0845
CPFKATP = 3.0
CPFKF16BP = 0.397
CPFKF26BP = 0.0174
CiPFKATP = 100.0
KACE = 0.5
KEQADH = 6.9e-05
KEQALD = 0.069
KEQENO = 6.7
KEQGLT = 1.0
KEQHK = 3800.0
KEQPGI = 0.314
KEQPGK = 3200.0
KEQPGM = 0.19
KEQTPI = 0.045
KGLY = 17.5
KIADHACALD = 1.1
KIADHETOH = 90.0
KIADHNAD = 0.92
KIADHNADH = 0.031
KIHKT6P = 0.04
KMADHACALD = 1.11
KMADHETOH = 17.0
KMADHNAD = 0.17
KMADHNADH = 0.11
KMALDDHAP = 2.4
KMALDF16P = 0.3
KMALDGAP = 2.0
KMALDGAPi = 10.0
KMENOP2G = 0.04
KMENOPEP = 0.5
KMGAPDHBPG = 0.51
KMGAPDHGAP = 0.39
KMGAPDHNAD = 2.85
KMGAPDHNADH = 0.007
KMGLTGLCi = 1.6
KMGLTGLCo = 1.6
KMHKADP = 0.23
KMHKATP = 0.15
KMHKG6P = 30.0
KMHKGLCi = 0.08
KMPDCPYR = 6.36
KMPFKATP = 0.71
KMPFKF6P = 0.1
KMPGIF6P = 0.3
KMPGIG6P = 1.4
KMPGKADP = 0.2
KMPGKATP = 0.3
KMPGKBPG = 0.003
KMPGKP3G = 0.53
KMPGMP2G = 0.08
KMPGMP3G = 1.2
KMPYKADP = 0.3
KMPYKATP = 9.3
KMPYKF16P = 0.2
KMPYKPEP = 0.19
KPFKAMP = 0.0995
KPFKF16BP = 0.111
KPFKF26BP = 0.000682
KSUC = 0.9
KTRE1 = 2.1
KTRE2 = 0.0
KiPFKATP = 0.65
L0 = 0.66
L10 = 60000.0
NADt = 1.59
NHPDC = 1.9
VMAXADH = 817.0
VMAXALD = 189.0
VMAXENO = 357.0
VMAXGAPDHf = 2090.0
VMAXGAPDHr = 1211.0
VMAXGLT = 220.0
VMAXHK = 285.0
VMAXPDC = 248.0
VMAXPFK = 213.0
VMAXPGI = 808.0
VMAXPGK = 2670.0
VMAXPGM = 856.0
VMAXPYK = 559.0
gR = 5.12
n10 = 4.0
ACE = 10.0
ADP = 1.0
AMP = 0.3
ATP = 5.0
ETOH = 25.0
F26BP = 0.014
GLCo = 50.0
GLY = 10.0
T6P = 2.2
TREH = 2.0
default_compartment = 1.0
external_compartment = 1.0


#initial values
ACALD = 0.04
BPG = 1e-05
F16P = 12.0
F6P = 0.74
G6P = 3.8
GLCi = 0.1
NADH = 0.29
P2G = 0.09
P3G = 0.69
PEP = 0.1
PYR = 2.8
TRIO = 1.0


#assignment rules