#queralt1

FIX: AA degr

#reactions
securinseparase_securindegradation:
 {1.0}securinseparase = $pool
  (kdsecurin + kadsecurin * Cdc20) * securinseparase
securinseparase_separase_degradation:
 {1.0}securinseparase = $pool
  kdseparase * securinseparase
v1:
 {1.0}AA = {1.0}Clb2
  ksclb2
v10:
 {1.0}separaseT = {1.0}degr
  kdseparase * separaseT
v11:
 $pool = {1.0}securinseparase
  lasecurin * securin * separase - ldsecurin * securinseparase
v13:
 $pool = {1.0}Net1
  (kad * Cdc14 + kd * PP2A) * (Net1P / (Jnet + Net1P))
v14:
 {1.0}Net1 = $pool
  (kp * Cdk + kap * MEN) * (Net1 / (Jnet + Net1 + Net1Cdc14))
v15:
 {1.0}Net1 = {1.0}Net1Cdc14
  lanet * Net1 * Cdc14
v16:
 {1.0}Net1Cdc14 = {1.0}Net1
  ldnet * Net1Cdc14
v17:
 {1.0}Net1Cdc14 = $pool
  (kp * Cdk + kap * MEN) * (Net1Cdc14 / (Jnet + Net1 + Net1Cdc14))
v18:
 {1.0}AA = {1.0}PoloT
  kspolo
v19:
 {1.0}PoloT = {1.0}degr
  (kdpolo + kadpolo * Cdh1) * PoloT
v2:
 {1.0}Clb2 = {1.0}degr
  (kdclb2 + kadclb2 * Cdc20 + kaadclb2 * Cdh1) * Clb2
v20:
 $pool = {1.0}Polo
  (kapolo + kaapolo * Cdk) * ((PoloT - Polo) / (Jpolo + PoloT - Polo))
v21:
 {1.0}Polo = $pool
  kipolo * (Polo / (Jpolo + Polo))
v22:
 {1.0}Polo = {1.0}degr
  (kdpolo + kadpolo * Cdh1) * Polo
v23:
 $pool = {1.0}Tem1
  (katem + kaatem * Polo) * ((Tem1T - Tem1) / (Jtem1 + Tem1T - Tem1))
v24:
 {1.0}Tem1 = $pool
  (kitem + kaitem * PP2A) * (Tem1 / (Jtem1 + Tem1))
v25:
 $pool = {1.0}Cdc15
  (kacdc15 + kaacdc15 * Cdc14) * ((Cdc15T - Cdc15) / (Jcdc15 + Cdc15T - Cdc15))
v26:
 {1.0}Cdc15 = $pool
  (kicdc15 + kaicdc15 * Cdk) * (Cdc15 / (Jcdc15 + Cdc15))
v27:
 {1.0}AA = {1.0}MEN
  lamen * (Tem1 - MEN) * (Cdc15 - MEN)
v28:
 {1.0}MEN = {1.0}degr
  ldmen * MEN
v29:
 {1.0}MEN = $pool
  (kitem + kaitem * PP2A) * (MEN / (Jtem1 + Tem1))
v3:
 {1.0}AA = {1.0}Cdc20
  kscdc20
v30:
 {1.0}MEN = $pool
  (kicdc15 + kaicdc15 * Cdk) * (MEN / (Jcdc15 + Cdc15))
v4:
 {1.0}Cdc20 = {1.0}degr
  (kdcdc20 + kadcdc20 * Cdh1) * Cdc20
v5:
 $pool = {1.0}Cdh1
  (kdcdh + kadcdh * Cdc14) * ((Cdh1T - Cdh1) / (Jcdh + Cdh1T - Cdh1))
v6:
 {1.0}Cdh1 = $pool
  kapcdh * Cdk * (Cdh1 / (Jcdh + Cdh1))
v7:
 {1.0}AA = {1.0}securinT
  kssecurin
v8:
 {1.0}securinT = {1.0}degr
  (kdsecurin + kadsecurin * Cdc20) * securinT
v9:
 {1.0}AA = {1.0}separaseT
  ksseparase


#parameters
Cdc14T = 0.5
Cdc15T = 1.0
Cdh1T = 1.0
Inh = 0.0
Jcdc15 = 0.2
Jcdh = 0.0015
Jnet = 0.2
Jpolo = 0.25
Jtem1 = 0.005
Net1T = 1.0
PP2AT = 1.0
Tem1T = 1.0
kaacdc15 = 0.5
kaadclb2 = 2.0
kaapolo = 0.5
kaatem = 0.5
kacdc15 = 0.02
kad = 0.1
kadcdc20 = 2.0
kadcdh = 1.0
kadclb2 = 0.2
kadpolo = 0.25
kadsecurin = 2.0
kaicdc15 = 0.2
kaitem = 1.0
kap = 2.0
kapcdh = 1.0
kapolo = 0.0
katem = 0.0
kd = 0.45
kdcdc20 = 0.05
kdcdh = 0.01
kdclb2 = 0.03
kdpolo = 0.01
kdsecurin = 0.05
kdseparase = 0.004
ki = 20.0
kicdc15 = 0.0
kipolo = 0.1
kitem = 0.1
kp = 0.4
kpp = 0.1
kscdc20 = 0.015
ksclb2 = 0.03
kspolo = 0.01
kssecurin = 0.03
ksseparase = 0.001
lamen = 10.0
lanet = 500.0
lasecurin = 500.0
ldmen = 0.1
ldnet = 1.0
ldsecurin = 1.0
AA = 1.0
degr = 1.0
compartment = 1.0


#initial values
Cdc15 = 0.0257
Cdc20 = 0.0
Cdh1 = 3.8e-05
Clb2 = 0.997
MEN = 8e-06
Net1 = 0.098
Net1Cdc14 = 0.485
Polo = 0.945
PoloT = 0.99
Tem1 = 0.00389
securinT = 0.6
securinseparase = 0.248
separaseT = 0.25


#assignment rules
!F PP2A = (1.0 + kpp * ki * separase) / (1.0 + ki * separase) * PP2AT
!F Cdc15_i = Cdc15T - Cdc15
!F Cdc14 = Cdc14T - Net1Cdc14
!F Net1P = Net1T - Net1 - Net1Cdc14
!F Cdk = Clb2 / (1.0 + Inh)
!F Polo_i = PoloT - Polo
!F securin = securinT - securinseparase
!F Tem1_i = Tem1T - Tem1
!F Cdh1_i = Cdh1T - Cdh1
!F separase = separaseT - securinseparase