begin name
pritchard1
end name

begin reactions
ADH {1.0}NAD + {1.0}$EtOH = {1.0}NADH + {1.0}AcAld
AK {2.0}ADP = {1.0}ATP + {1.0}AMP
ALD {1.0}F16bP = {1.0}DHAP + {1.0}GAP
ATPase {1.0}ATP = {1.0}ADP
ENO {1.0}P2G = {1.0}PEP
G3PDH {1.0}DHAP + {1.0}NADH = {1.0}NAD + {1.0}$Glycerol
GAPDH {1.0}GAP + {1.0}NAD = {1.0}BPG + {1.0}NADH
GlycogenBranch {1.0}ATP + {1.0}G6P = {1.0}ADP + {1.0}$Glycogen
HK {1.0}GLCi + {1.0}ATP = {1.0}G6P + {1.0}ADP
HXT {1.0}$GLCo = {1.0}GLCi
PDC {1.0}PYR = {1.0}AcAld + {1.0}$CO2
PFK {1.0}ATP + {1.0}F6P = {1.0}ADP + {1.0}F16bP
PGI {1.0}G6P = {1.0}F6P
PGK {1.0}ADP + {1.0}BPG = {1.0}ATP + {1.0}P3G
PGM {1.0}P3G = {1.0}P2G
PYK {1.0}ADP + {1.0}PEP = {1.0}ATP + {1.0}PYR
SuccinateBranch {3.0}NAD + {2.0}AcAld = {3.0}NADH + {1.0}$Succinate
TPI {1.0}DHAP = {1.0}GAP
TrehaloseBranch {1.0}ATP + {2.0}G6P = {1.0}ADP + {1.0}$Trehalose
end reactions

begin rate equations
ADH = (ADHVmax13*cell*((EtOH*NAD[t])/(ADHKetoh13*ADHKinad13) - (AcAld[t]*NADH[t])/(ADHKeq13*ADHKetoh13*ADHKinad13)))/(1 + (ADHKnad13*EtOH)/(ADHKetoh13*ADHKinad13) + (ADHKnadh13*AcAld[t])/(ADHKacald13*ADHKinadh13) + NAD[t]/ADHKinad13 + (EtOH*NAD[t])/(ADHKetoh13*ADHKinad13) + (ADHKnadh13*AcAld[t]*NAD[t])/(ADHKacald13*ADHKinadh13*ADHKinad13) + (EtOH*AcAld[t]*NAD[t])/(ADHKetoh13*ADHKiacald13*ADHKinad13) + NADH[t]/ADHKinadh13 + (ADHKnad13*EtOH*NADH[t])/(ADHKetoh13*ADHKinadh13*ADHKinad13) + (AcAld[t]*NADH[t])/(ADHKacald13*ADHKinadh13) + (EtOH*AcAld[t]*NADH[t])/(ADHKacald13*ADHKietoh13*ADHKinadh13))
AK = cell*(AKk115*ADP[t]^2 - AKk215*AMP[t]*ATP[t])
ALD = (ALDVmax5*cell*(F16bP[t]/ALDKf16bp5 - (DHAP[t]*GAP[t])/(ALDKeq5*ALDKf16bp5)))/(1 + DHAP[t]/ALDKdhap5 + F16bP[t]/ALDKf16bp5 + GAP[t]/ALDKgap5 + (DHAP[t]*GAP[t])/(ALDKdhap5*ALDKgap5) + (F16bP[t]*GAP[t])/(ALDKf16bp5*ALDKigap5))
ATPase = ATPaseKatpase14*cell*ATP[t]
ENO = (cell*ENOVmax10*(P2G[t]/ENOKp2g10 - PEP[t]/(ENOKeq10*ENOKp2g10)))/(1 + P2G[t]/ENOKp2g10 + PEP[t]/ENOKpep10)
G3PDH = (cell*G3PDHVmax16*(-((Glycerol*NAD[t])/(G3PDHKdhap16*G3PDHKeq16*G3PDHKnadh16)) + (DHAP[t]*NADH[t])/(G3PDHKdhap16*G3PDHKnadh16)))/((1 + Glycerol/G3PDHKglycerol16 + DHAP[t]/G3PDHKdhap16)*(1 + NAD[t]/G3PDHKnad16 + NADH[t]/G3PDHKnadh16))
GAPDH = (cell*GAPDHC7*((GAPDHVmaxf7*GAP[t]*NAD[t])/(GAPDHKgap7*GAPDHKnad7) - (GAPDHVmaxr7*BPG[t]*NADH[t])/(GAPDHKbpg7*GAPDHKnadh7)))/((1 + BPG[t]/GAPDHKbpg7 + GAP[t]/GAPDHKgap7)*(1 + NAD[t]/GAPDHKnad7 + NADH[t]/GAPDHKnadh7))
GlycogenBranch = cell*GlycogenBranchKGLYCOGEN17
HK = (cell*HKVmax2*(-((ADP[t]*G6P[t])/(HKKatp2*HKKeq2*HKKglc2)) + (ATP[t]*GLCi[t])/(HKKatp2*HKKglc2)))/((1 + ADP[t]/HKKadp2 + ATP[t]/HKKatp2)*(1 + G6P[t]/HKKg6p2 + GLCi[t]/HKKglc2))
HXT = (HXTVmax1*(GLCo - GLCi[t]))/(HXTKglc1*(1 + (GLCo*HXTKi1*GLCi[t])/HXTKglc1^2 + (GLCo + GLCi[t])/HXTKglc1))
PDC = (cell*PDCVmax12*(PYR[t]/PDCKpyr12)^PDCnH12)/(1 + (PYR[t]/PDCKpyr12)^PDCnH12)
PFK = (cell*PFKgR4*PFKVmax4*ATP[t]*F6P[t]*(1 + ATP[t]/PFKKatp4 + F6P[t]/PFKKf6p4 + (PFKgR4*ATP[t]*F6P[t])/(PFKKatp4*PFKKf6p4)))/(PFKKatp4*PFKKf6p4*((PFKL04*(1 + (PFKCamp4*AMP[t])/PFKKamp4)^2*(1 + (PFKCatp4*ATP[t])/PFKKatp4)^2*(1 + (PFKCiatp4*ATP[t])/PFKKiatp4)^2*(1 + (F26bP*PFKCf264)/PFKKf264 + (PFKCf164*F16bP[t])/PFKKf164)^2)/((1 + AMP[t]/PFKKamp4)^2*(1 + ATP[t]/PFKKiatp4)^2*(1 + F26bP/PFKKf264 + F16bP[t]/PFKKf164)^2) + (1 + ATP[t]/PFKKatp4 + F6P[t]/PFKKf6p4 + (PFKgR4*ATP[t]*F6P[t])/(PFKKatp4*PFKKf6p4))^2))
PGI = (cell*PGIVmax3*(-(F6P[t]/(PGIKeq3*PGIKg6p3)) + G6P[t]/PGIKg6p3))/(1 + F6P[t]/PGIKf6p3 + G6P[t]/PGIKg6p3)
PGK = (cell*PGKVmax8*(PGKKeq8*ADP[t]*BPG[t] - ATP[t]*P3G[t]))/(PGKKatp8*PGKKp3g8*(1 + ADP[t]/PGKKadp8 + ATP[t]/PGKKatp8)*(1 + BPG[t]/PGKKbpg8 + P3G[t]/PGKKp3g8))
PGM = (cell*PGMVmax9*(-(P2G[t]/(PGMKeq9*PGMKp3g9)) + P3G[t]/PGMKp3g9))/(1 + P2G[t]/PGMKp2g9 + P3G[t]/PGMKp3g9)
PYK = (cell*PYKVmax11*((ADP[t]*PEP[t])/(PYKKadp11*PYKKpep11) - (ATP[t]*PYR[t])/(PYKKadp11*PYKKeq11*PYKKpep11)))/((1 + ADP[t]/PYKKadp11 + ATP[t]/PYKKatp11)*(1 + PEP[t]/PYKKpep11 + PYR[t]/PYKKpyr11))
SuccinateBranch = cell*SuccinateBranchk19*AcAld[t]
TPI = cell*(TPIk16*DHAP[t] - TPIk26*GAP[t])
TrehaloseBranch = cell*TrehaloseBranchKtrehalose18
end rate equations

begin parameters
CO2 = 1.0
EtOH = 50.0
F26bP = 0.02
GLCo = 2.0
Glycerol = 0.15
Glycogen = 0.0
Succinate = 0.0
Trehalose = 0.0
AKk215 = 100.0
G3PDHVmax16 = 47.11
G3PDHKdhap16 = 0.4
G3PDHKnadh16 = 0.023
G3PDHKeq16 = 4300.0
HXTVmax1 = 97.24
HXTKglc1 = 1.1918
HXTKi1 = 0.91
HKVmax2 = 236.7
HKKglc2 = 0.08
HKKatp2 = 0.15
HKKeq2 = 2000.0
HKKg6p2 = 30.0
HKKadp2 = 0.23
PGIVmax3 = 1056.0
PGIKg6p3 = 1.4
PGIKeq3 = 0.29
PGIKf6p3 = 0.3
PFKVmax4 = 110.0
PFKgR4 = 5.12
PFKKf6p4 = 0.1
PFKKatp4 = 0.71
PFKL04 = 0.66
PFKCiatp4 = 100.0
PFKKiatp4 = 0.65
PFKCamp4 = 0.0845
PFKKamp4 = 0.0995
PFKCf264 = 0.0174
PFKKf264 = 0.000682
PFKCf164 = 0.397
PFKKf164 = 0.111
PFKCatp4 = 3.0
ALDVmax5 = 94.69
ALDKf16bp5 = 0.3
ALDKeq5 = 0.069
ALDKdhap5 = 2.0
ALDKgap5 = 2.4
ALDKigap5 = 10.0
TPIk16 = 450000.0
TPIk26 = 10000000.0
GAPDHC7 = 1.0
GAPDHVmaxf7 = 1152.0
GAPDHKgap7 = 0.21
GAPDHKnad7 = 0.09
GAPDHVmaxr7 = 6719.0
GAPDHKbpg7 = 0.0098
GAPDHKnadh7 = 0.06
PGKVmax8 = 1288.0
PGKKeq8 = 3200.0
PGKKp3g8 = 0.53
PGKKatp8 = 0.3
PGKKbpg8 = 0.003
PGKKadp8 = 0.2
PGMVmax9 = 2585.0
PGMKp3g9 = 1.2
PGMKeq9 = 0.19
PGMKp2g9 = 0.08
ENOVmax10 = 201.6
ENOKp2g10 = 0.04
ENOKeq10 = 6.7
ENOKpep10 = 0.5
PYKVmax11 = 1000.0
PYKKpep11 = 0.14
PYKKadp11 = 0.53
PYKKeq11 = 6500.0
PYKKpyr11 = 21.0
PYKKatp11 = 1.5
PDCVmax12 = 857.8
PDCKpyr12 = 4.33
PDCnH12 = 1.9
ADHVmax13 = 209.5
ADHKetoh13 = 17.0
ADHKinad13 = 0.92
ADHKeq13 = "6.9e-05"
ADHKnad13 = 0.17
ADHKnadh13 = 0.11
ADHKinadh13 = 0.031
ADHKacald13 = 1.11
ADHKiacald13 = 1.1
ADHKietoh13 = 90.0
ATPaseKatpase14 = 39.5
AKk115 = 45.0
G3PDHKglycerol16 = 1.0
G3PDHKnad16 = 0.93
GlycogenBranchKGLYCOGEN17 = 6.0
TrehaloseBranchKtrehalose18 = 2.4
SuccinateBranchk19 = 21.4
cell = 1.0
ext = 1.0
end parameters

begin initial conditions
ADP[0] = ADPi
AMP[0] = AMPi
ATP[0] = ATPi
AcAld[0] = AcAldi
BPG[0] = BPGi
DHAP[0] = DHAPi
F16bP[0] = F16bPi
F6P[0] = F6Pi
G6P[0] = G6Pi
GAP[0] = GAPi
GLCi[0] = GLCii
NAD[0] = NADi
NADH[0] = NADHi
P2G[0] = P2Gi
P3G[0] = P3Gi
PEP[0] = PEPi
PYR[0] = PYRi
end initial conditions

begin initial values
ADPi = 1.28198768168719
AMPi = 0.292884853320091
ATPi = 2.52512746499271
AcAldi = 0.178140579850657
BPGi = 0.000736873499865602
DHAPi = 1.00415254899644
F16bPi = 6.22132076069411
F6Pi = 0.624976405532373
G6Pi = 2.67504014044787
GAPi = 0.0451809175780963
GLCii = 0.097652231064563
NADi = 1.50329030201531
NADHi = 0.0867096979846952
P2Gi = 0.127695817386632
P3Gi = 0.885688538360659
PEPi = 0.0632352144936527
PYRi = 1.81531251192736
end initial values

begin assignment rules
end assignment rules

begin function definitions
end function definitions

begin events
end events

begin process annotations
ADH http://identifiers.org/kegg.reaction/R00754
ADH http://identifiers.org/ec-code/1.1.1.1
ADH http://identifiers.org/go/GO:0004022
ALD http://identifiers.org/kegg.reaction/R01068
ALD http://identifiers.org/ec-code/4.1.2.13
ALD http://identifiers.org/go/GO:0004332
ENO http://identifiers.org/kegg.reaction/R00658
ENO http://identifiers.org/ec-code/4.2.1.11
ENO http://identifiers.org/go/GO:0004634
GAPDH http://identifiers.org/kegg.reaction/R01061
GAPDH http://identifiers.org/ec-code/1.2.1.12
GAPDH http://identifiers.org/go/GO:0004365
HK http://identifiers.org/kegg.reaction/R02848
HK http://identifiers.org/ec-code/2.7.1.1
HK http://identifiers.org/go/GO:0004396
PDC http://identifiers.org/kegg.reaction/R00636
PDC http://identifiers.org/ec-code/4.1.1.1
PDC http://identifiers.org/go/GO:0004737
PGI http://identifiers.org/kegg.reaction/R00771
PGI http://identifiers.org/ec-code/5.3.1.9
PGI http://identifiers.org/go/GO:0004347
PGK http://identifiers.org/kegg.reaction/R01512
PGK http://identifiers.org/ec-code/2.7.2.3
PGK http://identifiers.org/go/GO:0004618
PYK http://identifiers.org/kegg.reaction/R00200
PYK http://identifiers.org/ec-code/2.7.1.40
PYK http://identifiers.org/go/GO:0004743
TPI http://identifiers.org/kegg.reaction/R01015
TPI http://identifiers.org/ec-code/5.3.1.1
TPI http://identifiers.org/go/GO:0004807
end process annotations

begin species annotations
ADP http://identifiers.org/chebi/CHEBI:16761
ADP http://identifiers.org/kegg.compound/C00008
AMP http://identifiers.org/chebi/CHEBI:16027
AMP http://identifiers.org/kegg.compound/C00020
ATP http://identifiers.org/chebi/CHEBI:15422
ATP http://identifiers.org/kegg.compound/C00002
AcAld http://identifiers.org/chebi/CHEBI:15343
AcAld http://identifiers.org/kegg.compound/C00084
BPG http://identifiers.org/chebi/CHEBI:16001
BPG http://identifiers.org/kegg.compound/C00236
CO2 http://identifiers.org/chebi/CHEBI:16526
CO2 http://identifiers.org/kegg.compound/C00011
DHAP http://identifiers.org/chebi/CHEBI:16108
DHAP http://identifiers.org/kegg.compound/C00111
EtOH http://identifiers.org/chebi/CHEBI:16236
EtOH http://identifiers.org/kegg.compound/C00469
F16bP http://identifiers.org/chebi/CHEBI:28013
F16bP http://identifiers.org/kegg.compound/C05378
F26bP http://identifiers.org/chebi/CHEBI:28602
F26bP http://identifiers.org/kegg.compound/C00665
F6P http://identifiers.org/chebi/CHEBI:16084
F6P http://identifiers.org/kegg.compound/C05345
G6P http://identifiers.org/chebi/CHEBI:17665
G6P http://identifiers.org/kegg.compound/C00668
GAP http://identifiers.org/chebi/CHEBI:29052
GAP http://identifiers.org/kegg.compound/C00118
GLCi http://identifiers.org/chebi/CHEBI:4167
GLCi http://identifiers.org/kegg.compound/C00031
GLCo http://identifiers.org/chebi/CHEBI:4167
GLCo http://identifiers.org/kegg.compound/C00031
Glycerol http://identifiers.org/chebi/CHEBI:17754
Glycerol http://identifiers.org/kegg.compound/C00116
Glycogen http://identifiers.org/chebi/CHEBI:28087
Glycogen http://identifiers.org/kegg.compound/C00182
NAD http://identifiers.org/chebi/CHEBI:15846
NAD http://identifiers.org/kegg.compound/C00003
NADH http://identifiers.org/chebi/CHEBI:16908
NADH http://identifiers.org/kegg.compound/C00004
P2G http://identifiers.org/chebi/CHEBI:17835
P2G http://identifiers.org/kegg.compound/C00631
P3G http://identifiers.org/chebi/CHEBI:17794
P3G http://identifiers.org/kegg.compound/C00197
PEP http://identifiers.org/chebi/CHEBI:18021
PEP http://identifiers.org/kegg.compound/C00074
PYR http://identifiers.org/chebi/CHEBI:15361
PYR http://identifiers.org/kegg.compound/C00022
Succinate http://identifiers.org/chebi/CHEBI:30031
Succinate http://identifiers.org/kegg.compound/C00042
Trehalose http://identifiers.org/chebi/CHEBI:16551
Trehalose http://identifiers.org/kegg.compound/C01083
end species annotations

begin units
end units