#nielsen

FIX: ADPo ATPo Glco NADHo NADo P

#reactions
v_1:
 {1.0}Glco = {1.0}Glc
  k0*Glco
v_10:
 {1.0}EtOH = {1.0}P
  k0*EtOH
v_11:
 {1.0}ACA = {1.0}P
  k0*ACA
v_12:
 {1.0}Pyr = {1.0}P
  k0*Pyr
v_13:
 {1.0}PEP = {1.0}P
  k0*PEP
v_14:
 {1.0}BPG = {1.0}P
  k0*BPG
v_15:
 {1.0}GAP = {1.0}P
  k0*GAP
v_16:
 {1.0}FBP = {1.0}P
  k0*FBP
v_17:
 {1.0}F6P = {1.0}P
  k0*F6P
v_18:
 {1.0}F6P = {1.0}P
  kf10*F6P
v_19:
 {1.0}Glc = {1.0}P
  k0*Glc
v_2:
 {1.0}Glc + {1.0}ATP = {1.0}F6P + {1.0}ADP
  V1m*ATP*Glc/(K1Glc+Glc)/(K1ATP+ATP)
v_20:
 {1.0}NADo = {1.0}NAD
  k0*NADo
v_21:
 {1.0}NAD = {1.0}P
  k0*NAD
v_22:
 {1.0}NADHo = {1.0}NADH
  k0*NADHo
v_23:
 {1.0}NADH = {1.0}P
  k0*NADH
v_24:
 {1.0}ADPo = {1.0}ADP
  k0*ADPo
v_25:
 {1.0}ADP = {1.0}P
  k0*ADP
v_26:
 {1.0}ATPo = {1.0}ATP
  k0*ATPo
v_27:
 {1.0}ATP = {1.0}P
  k0*ATP
v_28:
 {1.0}AMP = {1.0}P
  k0*AMP
v_29:
 {1.0}AMP + {1.0}ATP = {2.0}ADP
  kf9*AMP*ATP - kr9*ADP**2
v_3:
 {1.0}F6P + {1.0}ATP = {1.0}FBP + {1.0}ADP
  V2m*F6P**2/(K2+K2*kappa2*ATP**2/AMP**2+F6P**2)*(ATP/(K2mATP+ATP))
v_4:
 {1.0}FBP = {2.0}GAP
  k3f*FBP - k3b*GAP**2
v_5:
 {1.0}GAP + {1.0}NAD = {1.0}BPG + {1.0}NADH
  V4m*GAP*NAD/(K4G+GAP)/(K4N+NAD)
v_6:
 {1.0}BPG + {1.0}ADP = {1.0}PEP + {1.0}ATP
  k5f*BPG*ADP - k5r*PEP*ATP
v_7:
 {1.0}PEP + {1.0}ADP = {1.0}Pyr + {1.0}ATP
  V6m*ADP*PEP/(K6PEP+PEP)/(K6ADP+ADP)
v_8:
 {1.0}Pyr = {1.0}ACA
  V7m*Pyr/(K7+Pyr)
v_9:
 {1.0}ACA + {1.0}NADH = {1.0}EtOH + {1.0}NAD
  k8f*ACA*NADH - k8r*EtOH*NAD


#parameters
K1ATP = 0.063
K1Glc = 0.1
K2 = 0.0016
K2mATP = 0.01
K4G = 1.0
K4N = 1.0
K6ADP = 0.3
K6PEP = 0.2
K7 = 0.3
V1m = 0.5
V2m = 1.5
V4m = 10.0
V6m = 10.0
V7m = 2.0
k0 = 0.011
k3b = 50.0
k3f = 1.0
k5f = 1.0
k5r = 0.5
k8f = 1.0
k8r = 0.000143
kappa2 = 0.017
kf10 = 0.05
kf9 = 10.0
kr9 = 10.0
ADPo = 1.1
ATPo = 3.5
Glco = 50.0
NADHo = 0.24
NADo = 4.0
P = 0.0
default_compartment = 1.0


#initial values
ACA = 0.0738334
ADP = 0.108367
AMP = 0.00261149
ATP = 4.49064
BPG = 0.299109
EtOH = 0.339813
F6P = 0.65939
FBP = 0.00770135
GAP = 0.00190919
Glc = 0.0112817
NAD = 3.62057
NADH = 0.616118
PEP = 0.00211259
Pyr = 0.00422702


#assignment rules