begin name
nielsen
end name

begin reactions
v1 {1.0}$Glco = {1.0}Glc
v10 {1.0}EtOH = {1.0}$P
v11 {1.0}ACA = {1.0}$P
v12 {1.0}Pyr = {1.0}$P
v13 {1.0}PEP = {1.0}$P
v14 {1.0}BPG = {1.0}$P
v15 {1.0}GAP = {1.0}$P
v16 {1.0}FBP = {1.0}$P
v17 {1.0}F6P = {1.0}$P
v18 {1.0}F6P = {1.0}$P
v19 {1.0}Glc = {1.0}$P
v2 {1.0}Glc + {1.0}ATP = {1.0}F6P + {1.0}ADP
v20 {1.0}$NADo = {1.0}NAD
v21 {1.0}NAD = {1.0}$P
v22 {1.0}$NADHo = {1.0}NADH
v23 {1.0}NADH = {1.0}$P
v24 {1.0}$ADPo = {1.0}ADP
v25 {1.0}ADP = {1.0}$P
v26 {1.0}$ATPo = {1.0}ATP
v27 {1.0}ATP = {1.0}$P
v28 {1.0}AMP = {1.0}$P
v29 {1.0}AMP + {1.0}ATP = {2.0}ADP
v3 {1.0}F6P + {1.0}ATP = {1.0}FBP + {1.0}ADP
v4 {1.0}FBP = {2.0}GAP
v5 {1.0}GAP + {1.0}NAD = {1.0}BPG + {1.0}NADH
v6 {1.0}BPG + {1.0}ADP = {1.0}PEP + {1.0}ATP
v7 {1.0}PEP + {1.0}ADP = {1.0}Pyr + {1.0}ATP
v8 {1.0}Pyr = {1.0}ACA
v9 {1.0}ACA + {1.0}NADH = {1.0}EtOH + {1.0}NAD
end reactions

begin rate equations
v1 = Glco*k0
v10 = k0*EtOH[t]
v11 = k0*ACA[t]
v12 = k0*Pyr[t]
v13 = k0*PEP[t]
v14 = k0*BPG[t]
v15 = k0*GAP[t]
v16 = k0*FBP[t]
v17 = k0*F6P[t]
v18 = kf10*F6P[t]
v19 = k0*Glc[t]
v2 = (V1m*ATP[t]*Glc[t])/((K1ATP + ATP[t])*(K1Glc + Glc[t]))
v20 = k0*NADo
v21 = k0*NAD[t]
v22 = k0*NADHo
v23 = k0*NADH[t]
v24 = ADPo*k0
v25 = k0*ADP[t]
v26 = ATPo*k0
v27 = k0*ATP[t]
v28 = k0*AMP[t]
v29 = -(kr9*ADP[t]^2) + kf9*AMP[t]*ATP[t]
v3 = (V2m*ATP[t]*F6P[t]^2)/((K2mATP + ATP[t])*(K2 + (K2*kappa2*ATP[t]^2)/AMP[t]^2 + F6P[t]^2))
v4 = k3f*FBP[t] - k3b*GAP[t]^2
v5 = (V4m*GAP[t]*NAD[t])/((K4G + GAP[t])*(K4N + NAD[t]))
v6 = k5f*ADP[t]*BPG[t] - k5r*ATP[t]*PEP[t]
v7 = (V6m*ADP[t]*PEP[t])/((K6ADP + ADP[t])*(K6PEP + PEP[t]))
v8 = (V7m*Pyr[t])/(K7 + Pyr[t])
v9 = -(k8r*EtOH[t]*NAD[t]) + k8f*ACA[t]*NADH[t]
end rate equations

begin parameters
K1ATP = 0.063
K1Glc = 0.1
K2 = 0.0016
K2mATP = 0.01
K4G = 1.0
K4N = 1.0
K6ADP = 0.3
K6PEP = 0.2
K7 = 0.3
V1m = 0.5
V2m = 1.5
V4m = 10.0
V6m = 10.0
V7m = 2.0
k0 = 0.011
k3b = 50.0
k3f = 1.0
k5f = 1.0
k5r = 0.5
k8f = 1.0
k8r = 0.000143
kappa2 = 0.017
kf10 = 0.05
kf9 = 10.0
kr9 = 10.0
ADPo = 1.1
ATPo = 3.5
Glco = 50.0
NADHo = 0.24
NADo = 4.0
P = 0.0
defaultcompartment = 1.0
end parameters

begin initial conditions
ACA[0] = ACAi
ADP[0] = ADPi
AMP[0] = AMPi
ATP[0] = ATPi
BPG[0] = BPGi
EtOH[0] = EtOHi
F6P[0] = F6Pi
FBP[0] = FBPi
GAP[0] = GAPi
Glc[0] = Glci
NAD[0] = NADi
NADH[0] = NADHi
PEP[0] = PEPi
Pyr[0] = Pyri
end initial conditions

begin initial values
ACAi = 0.0738334
ADPi = 0.108367
AMPi = 0.00261149
ATPi = 4.49064
BPGi = 0.299109
EtOHi = 0.339813
F6Pi = 0.65939
FBPi = 0.00770135
GAPi = 0.00190919
Glci = 0.0112817
NADi = 3.62057
NADHi = 0.616118
PEPi = 0.00211259
Pyri = 0.00422702
end initial values

begin assignment rules
end assignment rules

begin function definitions
end function definitions

begin events
end events

begin process annotations
end process annotations

begin species annotations
ACA http://identifiers.org/obo.chebi/CHEBI%3A15343
ACA http://identifiers.org/kegg.compound/C00084
ADP http://identifiers.org/obo.chebi/CHEBI%3A16761
ADP http://identifiers.org/kegg.compound/C00008
ADPo http://identifiers.org/obo.chebi/CHEBI%3A16761
ADPo http://identifiers.org/kegg.compound/C00008
AMP http://identifiers.org/obo.chebi/CHEBI%3A16027
AMP http://identifiers.org/kegg.compound/C00020
ATP http://identifiers.org/obo.chebi/CHEBI%3A15422
ATP http://identifiers.org/kegg.compound/C00002
ATPo http://identifiers.org/obo.chebi/CHEBI%3A15422
ATPo http://identifiers.org/kegg.compound/C00002
BPG http://identifiers.org/obo.chebi/CHEBI%3A19324
EtOH http://identifiers.org/obo.chebi/CHEBI%3A16236
EtOH http://identifiers.org/kegg.compound/C00469
F6P http://identifiers.org/obo.chebi/CHEBI%3A15946
F6P http://identifiers.org/kegg.compound/C00085
FBP http://identifiers.org/obo.chebi/CHEBI%3A16905
FBP http://identifiers.org/kegg.compound/C00354
GAP http://identifiers.org/obo.chebi/CHEBI%3A29052
GAP http://identifiers.org/kegg.compound/C00118
Glc http://identifiers.org/kegg.compound/C00293
Glc http://identifiers.org/obo.chebi/CHEBI%3A17234
NADH http://identifiers.org/obo.chebi/CHEBI%3A16908
NADH http://identifiers.org/kegg.compound/C00004
NADHo http://identifiers.org/obo.chebi/CHEBI%3A16908
NADHo http://identifiers.org/kegg.compound/C00004
NADo http://identifiers.org/obo.chebi/CHEBI%3A13389
PEP http://identifiers.org/obo.chebi/CHEBI%3A44897
PEP http://identifiers.org/kegg.compound/C00074
Pyr http://identifiers.org/obo.chebi/CHEBI%3A32816
Pyr http://identifiers.org/kegg.compound/C00022
end species annotations

begin units
end units