#nakakuki1

FIX: EGF HRG

#reactions
re106:
 {1.0}ERK_c = {1.0}ERK_n
  function_4_v007(KexERK, KimERK, Vc, Vn, ERK_c, ERK_n)
re107:
 {1.0}pERK_c = {1.0}pERK_n
  function_4_v008(KexERKP, KimERKP, Vc, Vn, pERK_c, pERK_n)
re108:
 {1.0}ppERK_c = {1.0}ppERK_n
  function_4_v009(KexERKPP, KimERKPP, Vc, Vn, ppERK_c, ppERK_n)
re109:
 $pool = {1.0}PreDUSPmRNA
  nucleus * function_4_v010(K10, V10, n10, ppERK_n)
re110:
 {1.0}PreDUSPmRNA = {1.0}DUSPmRNA
  function_4_v011(Vn, p11, PreDUSPmRNA)
re111:
 {1.0}DUSP_c = {1.0}DUSP_n
  function_4_v018(KexDUSP, KimDUSP, Vc, Vn, DUSP_n, DUSP_c)
re112:
 {1.0}pDUSP_c = {1.0}pDUSP_n
  function_4_v019(KexDUSPP, KimDUSPP, Vc, Vn, pDUSP_c, pDUSP_n)
re113:
 {1.0}pRSK_c = {1.0}pRSK_n
  function_4_v026(KexRSKP, KimRSKP, Vc, Vn, pRSK_n, pRSK_c)
re115:
 {1.0}PreFOSmRNA = {1.0}c_FOSmRNA
  function_4_v032(Vn, p32, PreFOSmRNA)
re116:
 {1.0}c_FOS_c = {1.0}FOSn
  function_4_v040(KexFOS, KimFOS, Vc, Vn, c_FOS_c, FOSn)
re117:
 {1.0}pc_FOS_c = {1.0}FOSn_2
  function_4_v041(KexFOSP, KimFOSP, Vc, Vn, pc_FOS_c, FOSn_2)
re118:
 {1.0}PreFmRNA = {1.0}FmRNA
  function_4_v058(Vn, p58, PreFmRNA)
re119:
 {1.0}F = {1.0}Fn
  function_4_v062(KexF, KimF, Vc, Vn, F, Fn)
re85:
 {1.0}pERK_c = {1.0}ERK_c
  cytoplasm * function_4_v003(K3, K4, V3, pERK_c, ppERK_c)
re86:
 {1.0}ppERK_c = {1.0}pERK_c
  cytoplasm * function_4_v004(K3, K4, V4, pERK_c, ppERK_c)
re87:
 {1.0}pERK_n = {1.0}ERK_n
  nucleus * function_4_v005(K5, K6, V5, pERK_n, ppERK_n)
re88:
 {1.0}ppERK_n = {1.0}pERK_n
  nucleus * function_4_v006(K5, K6, V6, pERK_n, ppERK_n)
re89:
 $pool = {1.0}DUSP_c
  cytoplasm * function_4_v013(p13, DUSPmRNA)
re90:
 $pool = {1.0}c_FOS_c
  cytoplasm * function_4_v034(p34, c_FOSmRNA)
re92:
 $pool = {1.0}PreFmRNA
  nucleus * function_4_v057(K57, V57, n57, FOSn_2)
re94:
 {1.0}FmRNA = $pool
  cytoplasm * p59 * FmRNA
re95:
 $pool = {1.0}F
  cytoplasm * function_4_v060(p60, FmRNA)
re96:
 {1.0}F = $pool
  cytoplasm * p61 * F
re99:
 {1.0}Fn = $pool
  nucleus * function_4_v063(cytoplasm, nucleus, p63, Fn)
reaction_12:
 {1.0}DUSP_n = {1.0}pDUSP_n
  nucleus * function_4_v020(K20, V20, ppERK_n, DUSP_n)
reaction_13:
 {1.0}pDUSP_n = {1.0}DUSP_n
  nucleus * function_4_v021(K21, V21, pDUSP_n)
reaction_14:
 {1.0}DUSP_n = $pool
  nucleus * p22 * DUSP_n
reaction_15:
 {1.0}pDUSP_n = $pool
  nucleus * p23 * pDUSP_n
reaction_17:
 {1.0}DUSPmRNA = $pool
  cytoplasm * p12 * DUSPmRNA
reaction_21:
 {1.0}ppERK_n + {1.0}DUSP_n = {1.0}DUSP_n_ppERK_n
  nucleus * (p47 * DUSP_n * ppERK_n - m47 * DUSP_n_ppERK_n)
reaction_22:
 {1.0}DUSP_n_ppERK_n = {1.0}pERK_n + {1.0}DUSP_n
  nucleus * p48 * DUSP_n_ppERK_n
reaction_23:
 {1.0}pERK_n + {1.0}DUSP_n = {1.0}DUSP_n_pERK_n
  nucleus * (p49 * DUSP_n * pERK_n - m49 * DUSP_n_pERK_n)
reaction_24:
 {1.0}DUSP_n_pERK_n = {1.0}ERK_n + {1.0}DUSP_n
  nucleus * p50 * DUSP_n_pERK_n
reaction_25:
 {1.0}ppERK_n + {1.0}pDUSP_n = {1.0}pDUSP_n_ppERK_n
  nucleus * (p52 * pDUSP_n * ppERK_n - m52 * pDUSP_n_ppERK_n)
reaction_26:
 {1.0}pDUSP_n_ppERK_n = {1.0}pERK_n + {1.0}pDUSP_n
  nucleus * p53 * pDUSP_n_ppERK_n
reaction_27:
 {1.0}pERK_n + {1.0}pDUSP_n = {1.0}pDUSP_n_pERK_n
  nucleus * (p54 * pDUSP_n * pERK_n - m54 * pDUSP_n_pERK_n)
reaction_28:
 {1.0}pDUSP_n_pERK_n = {1.0}ERK_n + {1.0}pDUSP_n
  nucleus * p55 * pDUSP_n_pERK_n
reaction_29:
 {1.0}ERK_n + {1.0}DUSP_n = {1.0}DUSP_n_ERK_n
  nucleus * (p51 * DUSP_n * ERK_n - m51 * DUSP_n_ERK_n)
reaction_30:
 {1.0}ERK_n + {1.0}pDUSP_n = {1.0}pDUSP_n_ERK_n
  nucleus * (p56 * pDUSP_n * ERK_n - m56 * pDUSP_n_ERK_n)
reaction_31:
 {1.0}A1 = {1.0}A1_2
  EGFm * cytoplasm * function_4_v101(K101, V101, A1, EGF)
reaction_32:
 {1.0}ERK_c = {1.0}pERK_c
  cytoplasm * function_4_v001(Fct, K1, K2, V1, ERK_c, pERK_c, pMEK)
reaction_33:
 {1.0}DUSP_c = {1.0}pDUSP_c
  cytoplasm * function_4_v014(K14, V14, ppERK_c, DUSP_c)
reaction_34:
 {1.0}pDUSP_c = {1.0}DUSP_c
  cytoplasm * function_4_v015(K15, V15, pDUSP_c)
reaction_35:
 {1.0}DUSP_c = $pool
  cytoplasm * p16 * DUSP_c
reaction_36:
 {1.0}pDUSP_c = $pool
  cytoplasm * p17 * pDUSP_c
reaction_41:
 {1.0}RSK_c = {1.0}pRSK_c
  cytoplasm * function_4_v024(K24, V24, ppERK_c, RSK_c)
reaction_42:
 {1.0}pRSK_c = {1.0}RSK_c
  cytoplasm * function_4_v025(K25, V25, pRSK_c)
reaction_45:
 {1.0}CREB_n = {1.0}pCREB_n
  nucleus * function_4_v027(K27, V27, CREB_n, pRSK_n)
reaction_46:
 {1.0}pCREB_n = {1.0}CREB_n
  nucleus * function_4_v028(K28, V28, pCREB_n)
reaction_47:
 $pool = {1.0}PreFOSmRNA
  nucleus * function_4_v031(K31, KF31, V31, n31, nF31, Fn, pCREB_n, pElk1_n)
reaction_50:
 {1.0}c_FOSmRNA = $pool
  cytoplasm * p33 * c_FOSmRNA
reaction_52:
 {1.0}FOSn = {1.0}FOSn_2
  nucleus * function_4_v042(K42, V42, ppERK_n, FOSn)
reaction_53:
 {1.0}FOSn = {1.0}FOSn_2
  nucleus * function_4_v043(K43, V43, FOSn, pRSK_n)
reaction_54:
 {1.0}FOSn_2 = {1.0}FOSn
  nucleus * function_4_v044(K44, V44, FOSn_2)
reaction_55:
 {1.0}FOSn = $pool
  nucleus * p45 * FOSn
reaction_56:
 {1.0}FOSn_2 = $pool
  nucleus * p46 * FOSn_2
reaction_57:
 {1.0}Elk1_n = {1.0}pElk1_n
  nucleus * function_4_v029(K29, V29, ppERK_n, Elk1_n)
reaction_58:
 {1.0}pElk1_n = {1.0}Elk1_n
  nucleus * function_4_v030(K30, V30, pElk1_n)
reaction_59:
 {1.0}c_FOS_c = {1.0}pc_FOS_c
  cytoplasm * function_4_v035(K35, V35, c_FOS_c, ppERK_c)
reaction_6:
 {1.0}pERK_c = {1.0}ppERK_c
  cytoplasm * function_4_v002(Fct, K1, K2, V2, ERK_c, pERK_c, pMEK)
reaction_60:
 {1.0}pc_FOS_c = {1.0}c_FOS_c
  cytoplasm * function_4_v037(K37, V37, pc_FOS_c)
reaction_61:
 {1.0}c_FOS_c = $pool
  cytoplasm * p38 * c_FOS_c
reaction_62:
 {1.0}pc_FOS_c = $pool
  cytoplasm * p39 * pc_FOS_c
reaction_67:
 {1.0}c_FOS_c = {1.0}pc_FOS_c
  cytoplasm * function_4_v036(K36, V36, c_FOS_c, pRSK_c)
reaction_68:
 {1.0}A1_2 = {1.0}A1
  cytoplasm * function_4_v102(K102, V102, A1_2)
reaction_69:
 {1.0}A2 = {1.0}A2_2
  HRGm * cytoplasm * function_4_v103(K103, V103, A2, HRG)
reaction_70:
 {1.0}A2_2 = {1.0}A2
  cytoplasm * function_4_v104(K104, V104, A2_2)
reaction_71:
 {1.0}RsD = {1.0}RsT
  EGFm * cytoplasm * function_4_v105(K105, V105, EGF, RsD)
reaction_72:
 {1.0}RsD = {1.0}RsT
  HRGm * cytoplasm * function_4_v106(K106, V106, HRG, RsD)
reaction_73:
 {1.0}RsT = {1.0}RsD
  cytoplasm * function_4_v107(K107, V107, A1_2, RsT)
reaction_74:
 {1.0}RsT = {1.0}RsD
  cytoplasm * function_4_v108(K108, V108, A2_2, RsT)
reaction_75:
 {1.0}A3 = {1.0}A3_2
  HRGm * cytoplasm * function_4_v109(K109, V109, HRG, A3)
reaction_76:
 {1.0}A3_2 = {1.0}A3
  cytoplasm * function_4_v110(K110, V110, A3_2)
reaction_77:
 {1.0}Kin = {1.0}Kin_2
  HRGm * cytoplasm * function_4_v111(K111, V111, HRG, Kin)
reaction_78:
 {1.0}Kin_2 = {1.0}Kin
  cytoplasm * function_4_v112(K112, V112, A3_2, Kin_2)
reaction_79:
 {1.0}MEK = {1.0}pMEK
  cytoplasm * function_4_v113(K113, V113, RsT, MEK)
reaction_80:
 {1.0}MEK = {1.0}pMEK
  cytoplasm * function_4_v114(K114, V114, Kin_2, MEK)
reaction_81:
 {1.0}pMEK = {1.0}MEK
  cytoplasm * function_4_v115(K115, V115, pMEK)


#parameters
EGFm = 1.0
Fct = 0.7485
HRGm = 1.0
K1 = 307.041525298866
K10 = 169.0473748
K101 = 3475.168
K102 = 237.2001
K103 = 1334.132
K104 = 4046.71
K105 = 1.027895
K106 = 606.871
K107 = 424.6884
K108 = 11.5048
K109 = 7424.816
K110 = 425.5268
K111 = 858.3423
K112 = 4665.217
K113 = 20.50809
K114 = 7.774197
K115 = 2122.045
K14 = 34180.48
K15 = 0.001172165
K2 = 350.0
K20 = 735598.6967
K21 = 387.8377182
K24 = 29516.06587
K25 = 0.913939859
K27 = 441.5834425
K28 = 14.99180922
K29 = 21312.69109
K3 = 160.0
K30 = 15.04396629
K31 = 185.9760682
K35 = 8562.744184
K36 = 528.552427
K37 = 0.070379236
K4 = 60.0
K42 = 3992.061328
K43 = 1157.116021
K44 = 0.051168202
K5 = 29.94073716
K57 = 0.637490056
K6 = 29.9407371620698
KF31 = 0.013844393
KexDUSP = 0.070467899
KexDUSPP = 0.070467899
KexERK = 0.018
KexERKP = 0.018
KexERKPP = 0.013
KexF = 0.396950616
KexFOS = 0.133249762
KexFOSP = 0.133249762
KexRSKP = 0.129803956
KimDUSP = 0.024269764
KimDUSPP = 0.024269764
KimERK = 0.012
KimERKP = 0.012
KimERKPP = 0.011
KimF = 0.019898797
KimFOS = 0.54528521
KimFOSP = 0.54528521
KimRSKP = 0.025925065
V1 = 0.342848369838443
V10 = 29.24109258
V101 = 0.01807448
V102 = 0.09858154
V103 = 0.3573399
V104 = 4.635749
V105 = 0.05393704
V106 = 0.109304
V107 = 5.291093
V108 = 0.03436149
V109 = 0.1374307
V110 = 0.08258693
V111 = 0.02487469
V112 = 0.8850982
V113 = 0.05377297
V114 = 0.03957055
V115 = 13.74244
V14 = 5.636949216
V15 = 2.992346912
V2 = 0.22
V20 = 0.157678678
V21 = 0.005648117
V24 = 0.550346114
V25 = 10.09063736
V27 = 19.23118154
V28 = 6.574759504
V29 = 0.518529841
V3 = 0.72
V30 = 13.79479021
V31 = 0.655214248
V35 = 8.907637012
V36 = 0.000597315
V37 = 1.745848179
V4 = 0.648
V42 = 0.909968714
V43 = 0.076717457
V44 = 0.078344305
V5 = 19.49872346
V57 = 1.026834758
V6 = 19.4987234631759
Vc = 940.0
Vn = 220.0
m47 = 15.80783969
m49 = 2.335459127
m51 = 9.544308421
m52 = 15.80783969
m54 = 2.335459127
m56 = 9.544308421
n10 = 3.970849295
n31 = 1.988003164
n57 = 3.584464176
nF31 = 2.800340453
p11 = 0.000126129
p12 = 0.007875765
p13 = 0.001245747
p16 = 0.000257
p17 = 4.81e-05
p22 = 0.000257
p23 = 4.81e-05
p32 = 0.003284434
p33 = 0.000601234209304622
p34 = 7.64816282169636e-05
p38 = 0.000257
p39 = 4.81e-05
p45 = 0.000257
p46 = 4.81e-05
p47 = 0.001670815
p48 = 0.686020478
p49 = 0.314470502
p50 = 26.59483436
p51 = 0.01646825
p52 = 0.001670815
p53 = 0.686020478
p54 = 0.314470502
p55 = 26.59483436
p56 = 0.01646825
p58 = 0.000270488
p59 = 0.001443889
p60 = 0.002448164
p61 = 3.49860901414122e-05
p63 = 4.13466150826031e-05
EGF = 1.0
HRG = 1.0
cytoplasm = 940.0
default = 1.0
nucleus = 220.0


#initial values
A1 = 182.3524
A1_2 = 0.0
A2 = 25.38702
A2_2 = 0.0
A3 = 13.09262
A3_2 = 0.0
CREB_n = 1000.0
DUSP_c = 0.0
DUSP_n = 0.0
DUSP_n_ERK_n = 0.0
DUSP_n_pERK_n = 0.0
DUSP_n_ppERK_n = 0.0
DUSPmRNA = 0.0
ERK_c = 570.4179
ERK_n = 1624.9
Elk1_n = 1510.0
F = 0.0
FOSn = 0.0
FOSn_2 = 0.0
FmRNA = 0.0
Fn = 0.0
Kin = 82.66574
Kin_2 = 0.0
MEK = 637.3212
PreDUSPmRNA = 0.0
PreFOSmRNA = 0.0
PreFmRNA = 0.0
RSK_c = 353.0
RsD = 247.4035
RsT = 0.0
c_FOS_c = 0.0
c_FOSmRNA = 0.0
pCREB_n = 0.0
pDUSP_c = 0.0
pDUSP_n = 0.0
pDUSP_n_ERK_n = 0.0
pDUSP_n_pERK_n = 0.0
pDUSP_n_ppERK_n = 0.0
pERK_c = 0.0
pERK_n = 0.0
pElk1_n = 0.0
pMEK = 0.0
pRSK_c = 0.0
pRSK_n = 0.0
pc_FOS_c = 0.0
ppERK_c = 0.0
ppERK_n = 0.0


#assignment rules