(* Generated by JWS Online *) (* This is an experimental feature of JWS Online. Please report any mistakes.*) (* Note that the following notable SBML entities or features are not supported in notebook outputyet: *) (* Events *) (* Constraints *) (* Units and UnitDefinitions *) (* AlgebraicRules *) (* conversionFactors *) variables = { adp[t], atp[t], bpg13[t], dhap[t], f26bp[t], f6p[t], fbp[t], g6p[t], glc[t], grap[t], lac[t], pep[t], pg2[t], pg3[t], pyr[t] }; initialValues = { adp[0] == 1.199, atp[0] == 3.921, bpg13[0] == 0.018, dhap[0] == 0.011, f26bp[0] == 0.002, f6p[0] == 0.008, fbp[0] == 0.019, g6p[0] == 0.113, glc[0] == 2.571, grap[0] == 0.003, lac[0] == 1.083, pep[0] == 0.477, pg2[0] == 0.14, pg3[0] == 0.669, pyr[0] == 0.392 }; rates = { ATP\[LetterSpace]ADP\[LetterSpace]CHANGE, ATPconsumption, ATPformation, ENO, FBA, FBP, FBP26, GAPD, GLCt1r, HEX1, IRS, LDH\[LetterSpace]L, L\[LetterSpace]LACt2r, PFK, PFK26, PGI, PGK, PGM, PYK, PYRt2r, TPI }; rateEquations = { ATP\[LetterSpace]ADP\[LetterSpace]CHANGE -> (-atp\[LetterSpace]adp + atp\[LetterSpace]adp\[LetterSpace]ss)*cyto*NUCLEOTIDE\[LetterSpace]CHANGE\[LetterSpace]Vm, ATPconsumption -> (ATPconsumption\[LetterSpace]Vm*cyto*atp[t])/(ATPconsumption\[LetterSpace]KMatp*(1 + atp[t]/ATPconsumption\[LetterSpace]KMatp)), ATPformation -> (ATPformation\[LetterSpace]Vm*cyto*phos*adp[t])/(ATPformation\[LetterSpace]KMadp*ATPformation\[LetterSpace]KMphos*(1 + phos/ATPformation\[LetterSpace]KMphos)*(1 + adp[t]/ATPformation\[LetterSpace]KMadp)), ENO -> (cyto*ENO\[LetterSpace]Vm*(-(pep[t]/ENO\[LetterSpace]Keq) + pg2[t]))/(ENO\[LetterSpace]KMpg2*(1 + pep[t]/ENO\[LetterSpace]KMpep + pg2[t]/ENO\[LetterSpace]KMpg2)), FBA -> (cyto*FBA\[LetterSpace]Vm*(fbp[t] - (dhap[t]*grap[t])/FBA\[LetterSpace]Keq))/(FBA\[LetterSpace]KMfbp*(fbp[t]/FBA\[LetterSpace]KMfbp + (1 + dhap[t]/FBA\[LetterSpace]KMdhap)*(1 + grap[t]/FBA\[LetterSpace]KMgrap))), FBP -> (cyto*FBP\[LetterSpace]Vm*fbp[t])/(FBP\[LetterSpace]KMfbp*(1 + fbp[t]/FBP\[LetterSpace]KMfbp)), FBP26 -> (cyto*FBP26\[LetterSpace]Vm*f26bp[t])/(FBP26\[LetterSpace]KMf26bp*(1 + f26bp[t]/FBP26\[LetterSpace]KMf26bp)), GAPD -> (cyto*GAPD\[LetterSpace]Vm*(-((nadh*bpg13[t])/GAPD\[LetterSpace]Keq) + nad*phos*grap[t]))/(GAPD\[LetterSpace]KMgrap*GAPD\[LetterSpace]KMnad*GAPD\[LetterSpace]KMphos*(-1 + (1 + nadh/GAPD\[LetterSpace]KMnadh)*(1 + bpg13[t]/GAPD\[LetterSpace]KMbpg13) + (1 + nad/GAPD\[LetterSpace]KMnad)*(1 + phos/GAPD\[LetterSpace]KMphos)*(1 + grap[t]/GAPD\[LetterSpace]KMgrap))), GLCt1r -> (cyto*GLCt1r\[LetterSpace]Vm*(glc\[LetterSpace]ext - glc[t]/GLCt1r\[LetterSpace]Keq))/(GLCt1r\[LetterSpace]KMglc\[LetterSpace]ext*(1 + glc\[LetterSpace]ext/GLCt1r\[LetterSpace]KMglc\[LetterSpace]ext + glc[t]/GLCt1r\[LetterSpace]KMglc)), HEX1 -> (cyto*HEX1\[LetterSpace]Vm*(-((adp[t]*g6p[t])/HEX1\[LetterSpace]Keq) + atp[t]*glc[t]))/(HEX1\[LetterSpace]KMatp*HEX1\[LetterSpace]KMglc*(-1 + (1 + adp[t]/HEX1\[LetterSpace]KMadp)*(1 + g6p[t]/HEX1\[LetterSpace]KMg6p) + (1 + atp[t]/HEX1\[LetterSpace]KMatp)*(1 + glc[t]/HEX1\[LetterSpace]KMglc))), IRS -> cyto*(IRS\[LetterSpace]fp + IRS\[LetterSpace]ss), LDH\[LetterSpace]L -> (cyto*LDH\[LetterSpace]L\[LetterSpace]Vm*(-((nad*lac[t])/LDH\[LetterSpace]L\[LetterSpace]Keq) + nadh*pyr[t]))/(LDH\[LetterSpace]L\[LetterSpace]KMnadh*LDH\[LetterSpace]L\[LetterSpace]KMpyr*(-1 + (1 + nad/LDH\[LetterSpace]L\[LetterSpace]KMnad)*(1 + lac[t]/LDH\[LetterSpace]L\[LetterSpace]KMlac) + (1 + nadh/LDH\[LetterSpace]L\[LetterSpace]KMnadh)*(1 + pyr[t]/LDH\[LetterSpace]L\[LetterSpace]KMpyr))), L\[LetterSpace]LACt2r -> (cyto*L\[LetterSpace]LACt2r\[LetterSpace]Vm*lac[t])/(L\[LetterSpace]LACt2r\[LetterSpace]KMlac*(1 + lac[t]/L\[LetterSpace]LACt2r\[LetterSpace]KMlac)), PFK -> (cyto*PFK\[LetterSpace]Vm*atp[t]*f6p[t])/(PFK\[LetterSpace]KMatp*PFK\[LetterSpace]KMf6p*(1 + atp[t]/PFK\[LetterSpace]KMatp)*(1 + f6p[t]/PFK\[LetterSpace]KMf6p)), PFK26 -> (cyto*PFK26\[LetterSpace]Vm*atp[t]*f6p[t])/(PFK26\[LetterSpace]KMatp*PFK26\[LetterSpace]KMf6p*(1 + atp[t]/PFK26\[LetterSpace]KMatp)*(1 + f6p[t]/PFK26\[LetterSpace]KMf6p)), PGI -> (cyto*PGI\[LetterSpace]Vm*(-(f6p[t]/PGI\[LetterSpace]Keq) + g6p[t]))/(PGI\[LetterSpace]KMg6p*(1 + f6p[t]/PGI\[LetterSpace]KMf6p + g6p[t]/PGI\[LetterSpace]KMg6p)), PGK -> (cyto*PGK\[LetterSpace]Vm*(adp[t]*bpg13[t] - (atp[t]*pg3[t])/PGK\[LetterSpace]Keq))/(PGK\[LetterSpace]KMadp*PGK\[LetterSpace]KMbpg13*(-1 + (1 + adp[t]/PGK\[LetterSpace]KMadp)*(1 + bpg13[t]/PGK\[LetterSpace]KMbpg13) + (1 + atp[t]/PGK\[LetterSpace]KMatp)*(1 + pg3[t]/PGK\[LetterSpace]KMpg3))), PGM -> (cyto*PGM\[LetterSpace]Vm*(-(pg2[t]/PGM\[LetterSpace]Keq) + pg3[t]))/(PGM\[LetterSpace]KMpg3*(1 + pg2[t]/PGM\[LetterSpace]KMpg2 + pg3[t]/PGM\[LetterSpace]KMpg3)), PYK -> (cyto*PYK\[LetterSpace]Vm*(adp[t]*pep[t] - (atp[t]*pyr[t])/PYK\[LetterSpace]Keq))/(PYK\[LetterSpace]KMadp*PYK\[LetterSpace]KMpep*(-1 + (1 + adp[t]/PYK\[LetterSpace]KMadp)*(1 + pep[t]/PYK\[LetterSpace]KMpep) + (1 + atp[t]/PYK\[LetterSpace]KMatp)*(1 + pyr[t]/PYK\[LetterSpace]KMpyr))), PYRt2r -> (cyto*PYRt2r\[LetterSpace]Vm*pyr[t])/(PYRt2r\[LetterSpace]KMpyr*(1 + pyr[t]/PYRt2r\[LetterSpace]KMpyr)), TPI -> (cyto*TPI\[LetterSpace]Vm*(dhap[t] - grap[t]/TPI\[LetterSpace]Keq))/(TPI\[LetterSpace]KMdhap*(1 + dhap[t]/TPI\[LetterSpace]KMdhap + grap[t]/TPI\[LetterSpace]KMgrap)) }; parameters = { ATPconsumption\[LetterSpace]KMatp -> 2.0, ATPconsumption\[LetterSpace]Vm -> 5.58245, ATPformation\[LetterSpace]KMadp -> 5.6, ATPformation\[LetterSpace]KMphos -> 3.4, ATPformation\[LetterSpace]Vm -> 42.6819, ENO\[LetterSpace]KMpep -> 0.089, ENO\[LetterSpace]KMpg2 -> 0.3, ENO\[LetterSpace]Keq -> 4.38534496525434, ENO\[LetterSpace]Vm -> 191.263, FBA\[LetterSpace]KMdhap -> 0.38, FBA\[LetterSpace]KMfbp -> 0.027, FBA\[LetterSpace]KMgrap -> 0.32, FBA\[LetterSpace]Keq -> 0.000133163971260293, FBA\[LetterSpace]Vm -> 500.562, FBP26\[LetterSpace]KMf26bp -> 0.013, FBP26\[LetterSpace]Vm -> 8.05946, FBP\[LetterSpace]KMfbp -> 0.1, FBP\[LetterSpace]Vm -> 0.01, GAPD\[LetterSpace]KMbpg13 -> 0.018, GAPD\[LetterSpace]KMgrap -> 0.07, GAPD\[LetterSpace]KMnad -> 0.75, GAPD\[LetterSpace]KMnadh -> 0.012, GAPD\[LetterSpace]KMphos -> 3.4, GAPD\[LetterSpace]Keq -> 0.497383227406177, GAPD\[LetterSpace]Vm -> 4950.28, GLCt1r\[LetterSpace]KMglc -> 17.0, GLCt1r\[LetterSpace]KMglc\[LetterSpace]ext -> 17.0, GLCt1r\[LetterSpace]Keq -> 1.0, GLCt1r\[LetterSpace]Vm -> 1000.0, HEX1\[LetterSpace]KMadp -> 0.63, HEX1\[LetterSpace]KMatp -> 0.63, HEX1\[LetterSpace]KMg6p -> 19.9, HEX1\[LetterSpace]KMglc -> 5.0, HEX1\[LetterSpace]Keq -> 2255.52682196071, HEX1\[LetterSpace]Vm -> 2.71005, IRSfp\[LetterSpace]Kdatp\[LetterSpace]dt -> 0.0003, IRSfp\[LetterSpace]Vm -> 0.05, IRSss\[LetterSpace]Katp\[LetterSpace]ratio -> 5.13347666690148, IRSss\[LetterSpace]Vm -> 0.02, IRSss\[LetterSpace]hillatp\[LetterSpace]ratio -> 7.10527645078065, LDH\[LetterSpace]L\[LetterSpace]KMlac -> 3.0, LDH\[LetterSpace]L\[LetterSpace]KMnad -> 0.211, LDH\[LetterSpace]L\[LetterSpace]KMnadh -> 0.0107, LDH\[LetterSpace]L\[LetterSpace]KMpyr -> 0.398, LDH\[LetterSpace]L\[LetterSpace]Keq -> 100.0, LDH\[LetterSpace]L\[LetterSpace]Vm -> 7.26844, L\[LetterSpace]LACt2r\[LetterSpace]KMlac -> 0.8, L\[LetterSpace]LACt2r\[LetterSpace]Vm -> 5.21331, NUCLEOTIDE\[LetterSpace]CHANGE\[LetterSpace]Vm -> 0.2, PFK26\[LetterSpace]KMatp -> 0.328, PFK26\[LetterSpace]KMf6p -> 0.017, PFK26\[LetterSpace]Vm -> 4.19328, PFK\[LetterSpace]KMatp -> 0.063, PFK\[LetterSpace]KMf6p -> 0.35, PFK\[LetterSpace]Vm -> 7.48024, PGI\[LetterSpace]KMf6p -> 0.12, PGI\[LetterSpace]KMg6p -> 0.35, PGI\[LetterSpace]Keq -> 0.364659218171184, PGI\[LetterSpace]Vm -> 4525.64, PGK\[LetterSpace]KMadp -> 0.126, PGK\[LetterSpace]KMatp -> 0.24, PGK\[LetterSpace]KMbpg13 -> 0.042, PGK\[LetterSpace]KMpg3 -> 0.27, PGK\[LetterSpace]Keq -> 1931.283, PGK\[LetterSpace]Vm -> 4912.9, PGM\[LetterSpace]KMpg2 -> 0.139, PGM\[LetterSpace]KMpg3 -> 0.22, PGM\[LetterSpace]Keq -> 0.174472843901766, PGM\[LetterSpace]Vm -> 5000.0, PYK\[LetterSpace]KMadp -> 0.22, PYK\[LetterSpace]KMatp -> 0.86, PYK\[LetterSpace]KMpep -> 0.18, PYK\[LetterSpace]KMpyr -> 3.0, PYK\[LetterSpace]Keq -> 20593.3056361955, PYK\[LetterSpace]Vm -> 7.44078, PYRt2r\[LetterSpace]KMpyr -> 0.15, PYRt2r\[LetterSpace]Vm -> 0.0575902, TPI\[LetterSpace]KMdhap -> 0.62, TPI\[LetterSpace]KMgrap -> 0.55, TPI\[LetterSpace]Keq -> 0.113860326083997, TPI\[LetterSpace]Vm -> 4572.25, f\[LetterSpace]scale -> 1.0, presim\[LetterSpace]duration -> 60.0, presim\[LetterSpace]glc\[LetterSpace]ext -> 3.0, sim\[LetterSpace]glc\[LetterSpace]ext -> 3.0, cpep\[LetterSpace]ext -> 0.0, glc\[LetterSpace]ext -> 2.8, h -> 1.0, h2o -> 1.0, ins\[LetterSpace]ext -> 6*^-08, lac\[LetterSpace]ext -> 1.0, pyr\[LetterSpace]mito -> 1.0, cell -> 0.001321, mm -> 1.0, pm -> 1.0 }; assignments = { ext -> 1000*cell, cyto -> 0.95*cell, mito -> 0.05*cell, atp\[LetterSpace]adp -> adp[t] + atp[t], atp\[LetterSpace]ratio -> atp[t]/(1/100000000 + adp[t]), nadh\[LetterSpace]nad -> nad + nadh, nadh\[LetterSpace]ratio -> nadh/(1/100000000 + nad), fbp\[LetterSpace]grap -> fbp[t] + grap[t], fbp\[LetterSpace]dhap\[LetterSpace]grap -> dhap[t] + fbp[t] + grap[t], fbp\[LetterSpace]dhap -> dhap[t] + fbp[t], lac\[LetterSpace]pyr\[LetterSpace]ratio -> lac[t]/pyr[t], phos -> Piecewise[{{10.1746465657385, glc\[LetterSpace]ext < 2.4}, {10.1746465657385 - 0.184822580052604*(-2.4 + glc\[LetterSpace]ext), glc\[LetterSpace]ext >= 2.4 && glc\[LetterSpace]ext < 4}, {9.87893043765431 - 0.184822580052604*(-4 + glc\[LetterSpace]ext), glc\[LetterSpace]ext >= 4 && glc\[LetterSpace]ext < 8.6}, {9.02874656941233 - 0.184822580052604*(-8.6 + glc\[LetterSpace]ext), glc\[LetterSpace]ext >= 8.6 && glc\[LetterSpace]ext < 14.5}, {7.93829334710197 - 0.184822580052604*(-14.5 + glc\[LetterSpace]ext), glc\[LetterSpace]ext >= 14.5 && glc\[LetterSpace]ext < 16}, {7.66105947702306, glc\[LetterSpace]ext >= 16}}, 0], nad -> 0.59 + (0.3*glc\[LetterSpace]ext^1.39)/(10.20979845132981 + glc\[LetterSpace]ext^1.39), nadh -> 0.13 + (0.23*glc\[LetterSpace]ext^1.53)/(29.47951197523888 + glc\[LetterSpace]ext^1.53), datp\[LetterSpace]dt -> -ATPconsumption + ATPformation + ATP\[LetterSpace]ADP\[LetterSpace]CHANGE - HEX1 - PFK - PFK26 + PGK + PYK, IRS\[LetterSpace]per\[LetterSpace]vol -> IRS/cell, atp\[LetterSpace]adp\[LetterSpace]ss -> 4.66 + (1.87*glc\[LetterSpace]ext^2.03)/(16.764209380544166 + glc\[LetterSpace]ext^2.03), IRS\[LetterSpace]ss -> (IRSss\[LetterSpace]Vm*(atp[t]/adp[t])^IRSss\[LetterSpace]hillatp\[LetterSpace]ratio)/(IRSss\[LetterSpace]Katp\[LetterSpace]ratio^IRSss\[LetterSpace]hillatp\[LetterSpace]ratio + (atp[t]/adp[t])^IRSss\[LetterSpace]hillatp\[LetterSpace]ratio), IRS\[LetterSpace]fp -> Piecewise[{{(datp\[LetterSpace]dt*IRSfp\[LetterSpace]Vm)/(datp\[LetterSpace]dt + IRSfp\[LetterSpace]Kdatp\[LetterSpace]dt), datp\[LetterSpace]dt > 0}}, 0] }; events = { }; speciesAnnotations = { adp[t]->"http://identifiers.org/SBO:0000247", adp[t]->"http://identifiers.org/inchikey/XTWYTFMLZFPYCI-KQYNXXCUSA-N", adp[t]->"http://identifiers.org/vmhmetabolite/adp", adp[t]->"http://identifiers.org/bigg.metabolite/adp", adp[t]->"http://identifiers.org/kegg.compound/C00008", adp[t]->"http://identifiers.org/hmdb/HMDB01341", adp[t]->"http://identifiers.org/hmdb/HMDB0001341", adp[t]->"http://identifiers.org/biocyc/META:ADP", adp[t]->"http://identifiers.org/CHEBI:40553", adp[t]->"http://identifiers.org/CHEBI:456216", adp[t]->"http://identifiers.org/CHEBI:13222", adp[t]->"http://identifiers.org/CHEBI:16761", adp[t]->"http://identifiers.org/CHEBI:2342", adp[t]->"http://identifiers.org/CHEBI:87518", adp[t]->"http://identifiers.org/CHEBI:22244", adp[t]->"http://identifiers.org/metanetx.chemical/MNXM7", adp[t]->"http://identifiers.org/seed.compound/cpd00008", atp[t]->"http://identifiers.org/SBO:0000247", atp[t]->"http://identifiers.org/inchikey/ZKHQWZAMYRWXGA-KQYNXXCUSA-J", atp[t]->"http://identifiers.org/vmhmetabolite/atp", atp[t]->"http://identifiers.org/bigg.metabolite/atp", atp[t]->"http://identifiers.org/kegg.compound/C00002", atp[t]->"http://identifiers.org/hmdb/HMDB00538", atp[t]->"http://identifiers.org/hmdb/HMDB0000538", atp[t]->"http://identifiers.org/biocyc/META:ATP", atp[t]->"http://identifiers.org/CHEBI:30616", atp[t]->"http://identifiers.org/CHEBI:57299", atp[t]->"http://identifiers.org/CHEBI:40938", atp[t]->"http://identifiers.org/CHEBI:10841", atp[t]->"http://identifiers.org/CHEBI:10789", atp[t]->"http://identifiers.org/CHEBI:22249", atp[t]->"http://identifiers.org/CHEBI:2359", atp[t]->"http://identifiers.org/CHEBI:13236", atp[t]->"http://identifiers.org/CHEBI:15422", atp[t]->"http://identifiers.org/CHEBI:237958", atp[t]->"http://identifiers.org/metanetx.chemical/MNXM3", atp[t]->"http://identifiers.org/seed.compound/cpd00002", bpg13[t]->"http://identifiers.org/SBO:0000247", bpg13[t]->"http://identifiers.org/inchikey/LJQLQCAXBUHEAZ-UWTATZPHSA-N", bpg13[t]->"http://identifiers.org/vmhmetabolite/13dpg", bpg13[t]->"http://identifiers.org/bigg.metabolite/13dpg", bpg13[t]->"http://identifiers.org/kegg.compound/C00236", bpg13[t]->"http://identifiers.org/hmdb/HMDB62758", bpg13[t]->"http://identifiers.org/hmdb/HMDB0001270", bpg13[t]->"http://identifiers.org/biocyc/META:DPG", bpg13[t]->"http://identifiers.org/CHEBI:11881", bpg13[t]->"http://identifiers.org/CHEBI:57604", bpg13[t]->"http://identifiers.org/CHEBI:1658", bpg13[t]->"http://identifiers.org/CHEBI:20189", bpg13[t]->"http://identifiers.org/CHEBI:16001", bpg13[t]->"http://identifiers.org/metanetx.chemical/MNXM261", bpg13[t]->"http://identifiers.org/seed.compound/cpd00203", cpep\[LetterSpace]ext[t]->"http://identifiers.org/SBO:0000245", cpep\[LetterSpace]ext[t]->"http://identifiers.org/CHEBI:80332", cpep\[LetterSpace]ext[t]->"http://identifiers.org/ncit/C94608", dhap[t]->"http://identifiers.org/SBO:0000247", dhap[t]->"http://identifiers.org/inchikey/GNGACRATGGDKBX-UHFFFAOYSA-N", dhap[t]->"http://identifiers.org/vmhmetabolite/dhap", dhap[t]->"http://identifiers.org/bigg.metabolite/dhap", dhap[t]->"http://identifiers.org/kegg.compound/C00111", dhap[t]->"http://identifiers.org/hmdb/HMDB0001473", dhap[t]->"http://identifiers.org/hmdb/HMDB11735", dhap[t]->"http://identifiers.org/hmdb/HMDB01473", dhap[t]->"http://identifiers.org/biocyc/META:DIHYDROXY-ACETONE-PHOSPHATE", dhap[t]->"http://identifiers.org/CHEBI:24355", dhap[t]->"http://identifiers.org/CHEBI:16108", dhap[t]->"http://identifiers.org/CHEBI:14342", dhap[t]->"http://identifiers.org/CHEBI:57642", dhap[t]->"http://identifiers.org/CHEBI:5454", dhap[t]->"http://identifiers.org/CHEBI:39571", dhap[t]->"http://identifiers.org/CHEBI:14341", dhap[t]->"http://identifiers.org/metanetx.chemical/MNXM77", dhap[t]->"http://identifiers.org/seed.compound/cpd00095", f26bp[t]->"http://identifiers.org/SBO:0000247", f26bp[t]->"http://identifiers.org/inchikey/YXWOAJXNVLXPMU-ZXXMMSQZSA-N", f26bp[t]->"http://identifiers.org/vmhmetabolite/f26bp", f26bp[t]->"http://identifiers.org/bigg.metabolite/f26bp", f26bp[t]->"http://identifiers.org/kegg.compound/C00665", f26bp[t]->"http://identifiers.org/hmdb/HMDB01047", f26bp[t]->"http://identifiers.org/hmdb/HMDB0001047", f26bp[t]->"http://identifiers.org/biocyc/META:CPD-535", f26bp[t]->"http://identifiers.org/CHEBI:42586", f26bp[t]->"http://identifiers.org/CHEBI:20933", f26bp[t]->"http://identifiers.org/CHEBI:28602", f26bp[t]->"http://identifiers.org/CHEBI:12351", f26bp[t]->"http://identifiers.org/CHEBI:58579", f26bp[t]->"http://identifiers.org/CHEBI:4122", f26bp[t]->"http://identifiers.org/metanetx.chemical/MNXM651", f26bp[t]->"http://identifiers.org/seed.compound/cpd00503", f6p[t]->"http://identifiers.org/SBO:0000247", f6p[t]->"http://identifiers.org/inchikey/BGWGXPAPYGQALX-ARQDHWQXSA-N", f6p[t]->"http://identifiers.org/vmhmetabolite/f6p", f6p[t]->"http://identifiers.org/bigg.metabolite/f6p", f6p[t]->"http://identifiers.org/kegg.compound/C00085", f6p[t]->"http://identifiers.org/kegg.compound/C05345", f6p[t]->"http://identifiers.org/hmdb/HMDB03971", f6p[t]->"http://identifiers.org/hmdb/HMDB0000124", f6p[t]->"http://identifiers.org/biocyc/META:FRUCTOSE-6P", f6p[t]->"http://identifiers.org/CHEBI:16084", f6p[t]->"http://identifiers.org/CHEBI:42378", f6p[t]->"http://identifiers.org/CHEBI:10375", f6p[t]->"http://identifiers.org/CHEBI:57634", f6p[t]->"http://identifiers.org/CHEBI:22768", f6p[t]->"http://identifiers.org/CHEBI:12352", f6p[t]->"http://identifiers.org/metanetx.chemical/MNXM89621", f6p[t]->"http://identifiers.org/seed.compound/cpd19035", fbp[t]->"http://identifiers.org/SBO:0000247", fbp[t]->"http://identifiers.org/inchikey/RNBGYGVWRKECFJ-VRPWFDPXSA-N", fbp[t]->"http://identifiers.org/vmhmetabolite/fdp", fbp[t]->"http://identifiers.org/bigg.metabolite/fdp", fbp[t]->"http://identifiers.org/kegg.compound/C00354", fbp[t]->"http://identifiers.org/hmdb/HMDB0001058", fbp[t]->"http://identifiers.org/CHEBI:37736", fbp[t]->"http://identifiers.org/CHEBI:49299", fbp[t]->"http://identifiers.org/metanetx.chemical/MNXM417", fbp[t]->"http://identifiers.org/seed.compound/cpd00290", g6p[t]->"http://identifiers.org/SBO:0000247", g6p[t]->"http://identifiers.org/inchikey/YXWOAJXNVLXPMU-ZXXMMSQZSA-N", g6p[t]->"http://identifiers.org/vmhmetabolite/g6p", g6p[t]->"http://identifiers.org/bigg.metabolite/g6p", g6p[t]->"http://identifiers.org/kegg.compound/C00092", g6p[t]->"http://identifiers.org/hmdb/HMDB01401", g6p[t]->"http://identifiers.org/hmdb/HMDB06793", g6p[t]->"http://identifiers.org/hmdb/HMDB0001401", g6p[t]->"http://identifiers.org/hmdb/HMDB01549", g6p[t]->"http://identifiers.org/biocyc/META:D-glucopyranose-6-phosphate", g6p[t]->"http://identifiers.org/CHEBI:14314", g6p[t]->"http://identifiers.org/CHEBI:4170", g6p[t]->"http://identifiers.org/CHEBI:61548", g6p[t]->"http://identifiers.org/metanetx.chemical/MNXM160", g6p[t]->"http://identifiers.org/seed.compound/cpd26836", g6p[t]->"http://identifiers.org/seed.compound/cpd00079", glc[t]->"http://identifiers.org/SBO:0000247", glc[t]->"http://identifiers.org/inchikey/NBSCHQHZLSJFNQ-GASJEMHNSA-N", glc[t]->"http://identifiers.org/vmhmetabolite/glc\[LetterSpace]D", glc[t]->"http://identifiers.org/bigg.metabolite/glc\[LetterSpace]\[LetterSpace]D", glc[t]->"http://identifiers.org/kegg.compound/C00031", glc[t]->"http://identifiers.org/hmdb/HMDB0000122", glc[t]->"http://identifiers.org/hmdb/HMDB06564", glc[t]->"http://identifiers.org/hmdb/HMDB00122", glc[t]->"http://identifiers.org/biocyc/META:Glucopyranose", glc[t]->"http://identifiers.org/CHEBI:12965", glc[t]->"http://identifiers.org/CHEBI:20999", glc[t]->"http://identifiers.org/CHEBI:17634", glc[t]->"http://identifiers.org/CHEBI:4167", glc[t]->"http://identifiers.org/metanetx.chemical/MNXM41", glc[t]->"http://identifiers.org/seed.compound/cpd26821", glc[t]->"http://identifiers.org/seed.compound/cpd00027", 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h[t]->"http://identifiers.org/metanetx.chemical/MNXM1", h[t]->"http://identifiers.org/seed.compound/cpd00067", h2o[t]->"http://identifiers.org/SBO:0000247", h2o[t]->"http://identifiers.org/inchikey/XLYOFNOQVPJJNP-UHFFFAOYSA-M", h2o[t]->"http://identifiers.org/vmhmetabolite/h2o", h2o[t]->"http://identifiers.org/bigg.metabolite/h2o", h2o[t]->"http://identifiers.org/kegg.compound/C01328", h2o[t]->"http://identifiers.org/kegg.compound/C00001", h2o[t]->"http://identifiers.org/hmdb/HMDB01039", h2o[t]->"http://identifiers.org/hmdb/HMDB0002111", h2o[t]->"http://identifiers.org/hmdb/HMDB02111", h2o[t]->"http://identifiers.org/biocyc/META:CPD-15815", h2o[t]->"http://identifiers.org/biocyc/META:HYDROXYL-GROUP", h2o[t]->"http://identifiers.org/biocyc/META:WATER", h2o[t]->"http://identifiers.org/biocyc/META:OXONIUM", h2o[t]->"http://identifiers.org/biocyc/META:OH", h2o[t]->"http://identifiers.org/CHEBI:16234", h2o[t]->"http://identifiers.org/CHEBI:10743", 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lac[t]->"http://identifiers.org/biocyc/META:L-LACTATE", lac[t]->"http://identifiers.org/CHEBI:18783", lac[t]->"http://identifiers.org/CHEBI:11065", lac[t]->"http://identifiers.org/CHEBI:422", lac[t]->"http://identifiers.org/CHEBI:16651", lac[t]->"http://identifiers.org/CHEBI:12411", lac[t]->"http://identifiers.org/metanetx.chemical/MNXM179", lac[t]->"http://identifiers.org/seed.compound/cpd00159", lac\[LetterSpace]ext[t]->"http://identifiers.org/SBO:0000247", lac\[LetterSpace]ext[t]->"http://identifiers.org/inchikey/JVTAAEKCZFNVCJ-REOHCLBHSA-M", lac\[LetterSpace]ext[t]->"http://identifiers.org/vmhmetabolite/lac\[LetterSpace]L", lac\[LetterSpace]ext[t]->"http://identifiers.org/bigg.metabolite/lac\[LetterSpace]\[LetterSpace]L", lac\[LetterSpace]ext[t]->"http://identifiers.org/kegg.compound/C00186", lac\[LetterSpace]ext[t]->"http://identifiers.org/hmdb/HMDB03328", lac\[LetterSpace]ext[t]->"http://identifiers.org/hmdb/HMDB0000190", 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nadh[t]->"http://identifiers.org/hmdb/HMDB0001487", nadh[t]->"http://identifiers.org/hmdb/HMDB01487", nadh[t]->"http://identifiers.org/biocyc/META:NADH", nadh[t]->"http://identifiers.org/CHEBI:57945", nadh[t]->"http://identifiers.org/CHEBI:21902", nadh[t]->"http://identifiers.org/CHEBI:44216", nadh[t]->"http://identifiers.org/CHEBI:7423", nadh[t]->"http://identifiers.org/CHEBI:13395", nadh[t]->"http://identifiers.org/CHEBI:13396", nadh[t]->"http://identifiers.org/CHEBI:16908", nadh[t]->"http://identifiers.org/metanetx.chemical/MNXM10", nadh[t]->"http://identifiers.org/seed.compound/cpd00004", pep[t]->"http://identifiers.org/SBO:0000247", pep[t]->"http://identifiers.org/inchikey/DTBNBXWJWCWCIK-UHFFFAOYSA-M", pep[t]->"http://identifiers.org/vmhmetabolite/pep", pep[t]->"http://identifiers.org/bigg.metabolite/pep", pep[t]->"http://identifiers.org/kegg.compound/C00074", pep[t]->"http://identifiers.org/hmdb/HMDB0000263", pep[t]->"http://identifiers.org/hmdb/HMDB00263", pep[t]->"http://identifiers.org/biocyc/META:PHOSPHO-ENOL-PYRUVATE", pep[t]->"http://identifiers.org/CHEBI:8147", pep[t]->"http://identifiers.org/CHEBI:18021", pep[t]->"http://identifiers.org/CHEBI:44897", pep[t]->"http://identifiers.org/CHEBI:58702", pep[t]->"http://identifiers.org/CHEBI:14812", pep[t]->"http://identifiers.org/CHEBI:26055", pep[t]->"http://identifiers.org/CHEBI:44894", pep[t]->"http://identifiers.org/CHEBI:26054", pep[t]->"http://identifiers.org/metanetx.chemical/MNXM73", pep[t]->"http://identifiers.org/seed.compound/cpd00061", pg2[t]->"http://identifiers.org/SBO:0000247", pg2[t]->"http://identifiers.org/inchikey/GXIURPTVHJPJLF-UWTATZPHSA-K", pg2[t]->"http://identifiers.org/vmhmetabolite/2pg", pg2[t]->"http://identifiers.org/bigg.metabolite/2pg", pg2[t]->"http://identifiers.org/kegg.compound/C00631", pg2[t]->"http://identifiers.org/hmdb/HMDB03391", pg2[t]->"http://identifiers.org/hmdb/HMDB00362", pg2[t]->"http://identifiers.org/hmdb/HMDB0003391", pg2[t]->"http://identifiers.org/hmdb/HMDB62707", pg2[t]->"http://identifiers.org/biocyc/META:2-PG", pg2[t]->"http://identifiers.org/CHEBI:58289", pg2[t]->"http://identifiers.org/CHEBI:39868", pg2[t]->"http://identifiers.org/CHEBI:11651", pg2[t]->"http://identifiers.org/CHEBI:24344", pg2[t]->"http://identifiers.org/CHEBI:17835", pg2[t]->"http://identifiers.org/CHEBI:12986", pg2[t]->"http://identifiers.org/CHEBI:21028", pg2[t]->"http://identifiers.org/CHEBI:1267", pg2[t]->"http://identifiers.org/CHEBI:88350", pg2[t]->"http://identifiers.org/metanetx.chemical/MNXM275", pg2[t]->"http://identifiers.org/seed.compound/cpd00482", pg3[t]->"http://identifiers.org/SBO:0000247", pg3[t]->"http://identifiers.org/inchikey/OSJPPGNTCRNQQC-UWTATZPHSA-N", pg3[t]->"http://identifiers.org/vmhmetabolite/3pg", pg3[t]->"http://identifiers.org/bigg.metabolite/3pg", pg3[t]->"http://identifiers.org/kegg.compound/C00197", pg3[t]->"http://identifiers.org/hmdb/HMDB60180", pg3[t]->"http://identifiers.org/hmdb/HMDB0060180", pg3[t]->"http://identifiers.org/biocyc/META:G3P", pg3[t]->"http://identifiers.org/CHEBI:11879", pg3[t]->"http://identifiers.org/CHEBI:17794", pg3[t]->"http://identifiers.org/CHEBI:12987", pg3[t]->"http://identifiers.org/CHEBI:58272", pg3[t]->"http://identifiers.org/CHEBI:11880", pg3[t]->"http://identifiers.org/CHEBI:21029", pg3[t]->"http://identifiers.org/CHEBI:1657", pg3[t]->"http://identifiers.org/metanetx.chemical/MNXM126", pg3[t]->"http://identifiers.org/seed.compound/cpd00169", phos[t]->"http://identifiers.org/SBO:0000247", phos[t]->"http://identifiers.org/inchikey/NBIIXXVUZAFLBC-UHFFFAOYSA-K", phos[t]->"http://identifiers.org/vmhmetabolite/pi", phos[t]->"http://identifiers.org/bigg.metabolite/pi", phos[t]->"http://identifiers.org/kegg.compound/C00009", phos[t]->"http://identifiers.org/hmdb/HMDB02105", phos[t]->"http://identifiers.org/hmdb/HMDB00973", phos[t]->"http://identifiers.org/hmdb/HMDB05947", phos[t]->"http://identifiers.org/hmdb/HMDB0001429", phos[t]->"http://identifiers.org/hmdb/HMDB02142", phos[t]->"http://identifiers.org/hmdb/HMDB01429", phos[t]->"http://identifiers.org/biocyc/META:PHOSPHATE-GROUP", phos[t]->"http://identifiers.org/biocyc/META:CPD-16459", phos[t]->"http://identifiers.org/biocyc/META:Pi", phos[t]->"http://identifiers.org/CHEBI:26020", phos[t]->"http://identifiers.org/CHEBI:45024", phos[t]->"http://identifiers.org/CHEBI:18367", phos[t]->"http://identifiers.org/CHEBI:39745", phos[t]->"http://identifiers.org/CHEBI:7793", phos[t]->"http://identifiers.org/CHEBI:14791", phos[t]->"http://identifiers.org/CHEBI:43470", phos[t]->"http://identifiers.org/CHEBI:29139", phos[t]->"http://identifiers.org/CHEBI:26078", phos[t]->"http://identifiers.org/CHEBI:39739", phos[t]->"http://identifiers.org/CHEBI:43474", phos[t]->"http://identifiers.org/CHEBI:35780", phos[t]->"http://identifiers.org/CHEBI:29137", phos[t]->"http://identifiers.org/metanetx.chemical/MNXM9", phos[t]->"http://identifiers.org/seed.compound/cpd00009", phos[t]->"http://identifiers.org/seed.compound/cpd27787", pyr[t]->"http://identifiers.org/SBO:0000247", pyr[t]->"http://identifiers.org/inchikey/LCTONWCANYUPML-UHFFFAOYSA-M", pyr[t]->"http://identifiers.org/vmhmetabolite/pyr", pyr[t]->"http://identifiers.org/bigg.metabolite/pyr", pyr[t]->"http://identifiers.org/kegg.compound/C00022", pyr[t]->"http://identifiers.org/hmdb/HMDB0000243", pyr[t]->"http://identifiers.org/hmdb/HMDB00243", pyr[t]->"http://identifiers.org/biocyc/META:PYRUVATE", pyr[t]->"http://identifiers.org/CHEBI:15361", pyr[t]->"http://identifiers.org/CHEBI:32816", pyr[t]->"http://identifiers.org/CHEBI:26466", pyr[t]->"http://identifiers.org/CHEBI:45253", pyr[t]->"http://identifiers.org/CHEBI:8685", pyr[t]->"http://identifiers.org/CHEBI:14987", pyr[t]->"http://identifiers.org/CHEBI:26462", pyr[t]->"http://identifiers.org/metanetx.chemical/MNXM23", pyr[t]->"http://identifiers.org/seed.compound/cpd00020", pyr\[LetterSpace]mito[t]->"http://identifiers.org/SBO:0000247", pyr\[LetterSpace]mito[t]->"http://identifiers.org/inchikey/LCTONWCANYUPML-UHFFFAOYSA-M", pyr\[LetterSpace]mito[t]->"http://identifiers.org/vmhmetabolite/pyr", pyr\[LetterSpace]mito[t]->"http://identifiers.org/bigg.metabolite/pyr", pyr\[LetterSpace]mito[t]->"http://identifiers.org/kegg.compound/C00022", pyr\[LetterSpace]mito[t]->"http://identifiers.org/hmdb/HMDB0000243", pyr\[LetterSpace]mito[t]->"http://identifiers.org/hmdb/HMDB00243", pyr\[LetterSpace]mito[t]->"http://identifiers.org/biocyc/META:PYRUVATE", pyr\[LetterSpace]mito[t]->"http://identifiers.org/CHEBI:15361", pyr\[LetterSpace]mito[t]->"http://identifiers.org/CHEBI:32816", pyr\[LetterSpace]mito[t]->"http://identifiers.org/CHEBI:26466", pyr\[LetterSpace]mito[t]->"http://identifiers.org/CHEBI:45253", pyr\[LetterSpace]mito[t]->"http://identifiers.org/CHEBI:8685", pyr\[LetterSpace]mito[t]->"http://identifiers.org/CHEBI:14987", pyr\[LetterSpace]mito[t]->"http://identifiers.org/CHEBI:26462", pyr\[LetterSpace]mito[t]->"http://identifiers.org/metanetx.chemical/MNXM23", pyr\[LetterSpace]mito[t]->"http://identifiers.org/seed.compound/cpd00020" }; reactionAnnotations = { ATP\[LetterSpace]ADP\[LetterSpace]CHANGE->"http://identifiers.org/SBO:0000176", ATPconsumption->"http://identifiers.org/SBO:0000176", ATPformation->"http://identifiers.org/SBO:0000176", ENO->"http://identifiers.org/SBO:0000176", ENO->"http://identifiers.org/vmhreaction/ENO", ENO->"http://identifiers.org/rhea/10167", ENO->"http://identifiers.org/rhea/10164", ENO->"http://identifiers.org/metanetx.reaction/MNXR97932", ENO->"http://identifiers.org/seed/rxn00459", ENO->"http://identifiers.org/bigg.reaction/ENO", ENO->"http://identifiers.org/biocyc/META:2PGADEHYDRAT-RXN", ENO->"http://identifiers.org/ec-code/4.2.1.11", ENO->"http://identifiers.org/kegg.reaction/R00658", ENO->"http://identifiers.org/uniprot/P06733", ENO->"http://identifiers.org/uniprot/P13929", ENO->"http://identifiers.org/uniprot/P09104", FBA->"http://identifiers.org/SBO:0000176", FBA->"http://identifiers.org/vmhreaction/FBA", FBA->"http://identifiers.org/rhea/14729", FBA->"http://identifiers.org/rhea/14732", FBA->"http://identifiers.org/metanetx.reaction/MNXR99459", FBA->"http://identifiers.org/seed/rxn00786", FBA->"http://identifiers.org/bigg.reaction/FBA", FBA->"http://identifiers.org/ec-code/4.1.2.13", FBA->"http://identifiers.org/kegg.reaction/R01068", FBA->"http://identifiers.org/uniprot/P09972", FBA->"http://identifiers.org/uniprot/P04075", FBA->"http://identifiers.org/uniprot/P05062", FBP->"http://identifiers.org/SBO:0000176", FBP->"http://identifiers.org/vmhreaction/FBP", FBP->"http://identifiers.org/metanetx.reaction/MNXR99465", FBP->"http://identifiers.org/seed/rxn00549", FBP->"http://identifiers.org/bigg.reaction/FBP", FBP->"http://identifiers.org/ec-code/3.1.3.11", FBP->"http://identifiers.org/rhea/11064", FBP->"http://identifiers.org/uniprot/P09467", FBP->"http://identifiers.org/uniprot/Q9UMQ9", FBP->"http://identifiers.org/uniprot/O00757", FBP->"http://identifiers.org/uniprot/Q9UJ73", FBP->"http://identifiers.org/uniprot/Q9UMR0", FBP26->"http://identifiers.org/SBO:0000176", FBP26->"http://identifiers.org/vmhreaction/FBP26", FBP26->"http://identifiers.org/metanetx.reaction/MNXR99466", FBP26->"http://identifiers.org/bigg.reaction/FBP26", FBP26->"http://identifiers.org/biocyc/META:3.1.3.46-RXN", FBP26->"http://identifiers.org/ec-code/3.1.3.46", FBP26->"http://identifiers.org/kegg.reaction/R02731", FBP26->"http://identifiers.org/rhea/17289", FBP26->"http://identifiers.org/uniprot/Q16875", FBP26->"http://identifiers.org/uniprot/Q9NQ88", FBP26->"http://identifiers.org/uniprot/Q16877", FBP26->"http://identifiers.org/uniprot/P16118", FBP26->"http://identifiers.org/uniprot/O60825", GAPD->"http://identifiers.org/SBO:0000176", GAPD->"http://identifiers.org/vmhreaction/GAPD", GAPD->"http://identifiers.org/rhea/10303", GAPD->"http://identifiers.org/rhea/10300", GAPD->"http://identifiers.org/metanetx.reaction/MNXR100040", GAPD->"http://identifiers.org/seed/rxn00781", GAPD->"http://identifiers.org/bigg.reaction/GAPD", GAPD->"http://identifiers.org/biocyc/META:GAPOXNPHOSPHN-RXN", GAPD->"http://identifiers.org/ec-code/1.2.1.12", GAPD->"http://identifiers.org/kegg.reaction/R01061", GAPD->"http://identifiers.org/uniprot/P04406", GAPD->"http://identifiers.org/uniprot/O14556", GLCt1r->"http://identifiers.org/SBO:0000655", GLCt1r->"http://identifiers.org/vmhreaction/GLCt1r", GLCt1r->"http://identifiers.org/bigg.reaction/GLCt1", GLCt1r->"http://identifiers.org/biocyc/META:TRANS-RXN0-574", GLCt1r->"http://identifiers.org/metanetx.reaction/MNXR100188", HEX1->"http://identifiers.org/SBO:0000176", HEX1->"http://identifiers.org/vmhreaction/HEX1", HEX1->"http://identifiers.org/rhea/17825", HEX1->"http://identifiers.org/rhea/17828", HEX1->"http://identifiers.org/metanetx.reaction/MNXR100612", HEX1->"http://identifiers.org/seed/rxn00216", HEX1->"http://identifiers.org/bigg.reaction/HEX1", HEX1->"http://identifiers.org/biocyc/META:GLUCOKIN-RXN", HEX1->"http://identifiers.org/ec-code/2.7.1.1", HEX1->"http://identifiers.org/kegg.reaction/R00299", HEX1->"http://identifiers.org/uniprot/P35557", IRS->"http://identifiers.org/SBO:0000655", IRS->"http://identifiers.org/GO:0030073", LDH\[LetterSpace]L->"http://identifiers.org/SBO:0000176", LDH\[LetterSpace]L->"http://identifiers.org/vmhreaction/LDH\[LetterSpace]L", LDH\[LetterSpace]L->"http://identifiers.org/rhea/23444", LDH\[LetterSpace]L->"http://identifiers.org/rhea/23447", LDH\[LetterSpace]L->"http://identifiers.org/metanetx.reaction/MNXR101040", LDH\[LetterSpace]L->"http://identifiers.org/seed/rxn00499", LDH\[LetterSpace]L->"http://identifiers.org/bigg.reaction/LDH\[LetterSpace]L", LDH\[LetterSpace]L->"http://identifiers.org/biocyc/META:L-LACTATE-DEHYDROGENASE-RXN", LDH\[LetterSpace]L->"http://identifiers.org/ec-code/1.1.1.27", LDH\[LetterSpace]L->"http://identifiers.org/kegg.reaction/R00703", LDH\[LetterSpace]L->"http://identifiers.org/uniprot/P00338", LDH\[LetterSpace]L->"http://identifiers.org/uniprot/P07864", LDH\[LetterSpace]L->"http://identifiers.org/uniprot/P07195", LDH\[LetterSpace]L->"http://identifiers.org/uniprot/Q9BYZ2", L\[LetterSpace]LACt2r->"http://identifiers.org/SBO:0000655", L\[LetterSpace]LACt2r->"http://identifiers.org/vmhreaction/L\[LetterSpace]LACt2r", L\[LetterSpace]LACt2r->"http://identifiers.org/metanetx.reaction/MNXR101277", L\[LetterSpace]LACt2r->"http://identifiers.org/seed/rxn05602", L\[LetterSpace]LACt2r->"http://identifiers.org/bigg.reaction/L\[LetterSpace]LACt2r", L\[LetterSpace]LACt2r->"http://identifiers.org/biocyc/META:TRANS-RXN-104", L\[LetterSpace]LACt2r->"http://identifiers.org/rhea/29415", PFK->"http://identifiers.org/SBO:0000176", PFK->"http://identifiers.org/vmhreaction/PFK", PFK->"http://identifiers.org/metanetx.reaction/MNXR102507", PFK->"http://identifiers.org/seed/rxn00545", PFK->"http://identifiers.org/bigg.reaction/PFK", PFK->"http://identifiers.org/ec-code/2.7.1.11", PFK->"http://identifiers.org/rhea/16109", PFK->"http://identifiers.org/uniprot/P17858", PFK->"http://identifiers.org/uniprot/P08237", PFK->"http://identifiers.org/uniprot/Q01813", PFK26->"http://identifiers.org/SBO:0000176", PFK26->"http://identifiers.org/vmhreaction/PFK26", PFK26->"http://identifiers.org/rhea/15656", PFK26->"http://identifiers.org/rhea/15653", PFK26->"http://identifiers.org/metanetx.reaction/MNXR102508", PFK26->"http://identifiers.org/seed/rxn01971", PFK26->"http://identifiers.org/bigg.reaction/PFK26", PFK26->"http://identifiers.org/biocyc/META:6-PHOSPHOFRUCTO-2-KINASE-RXN", PFK26->"http://identifiers.org/ec-code/2.7.1.105", PFK26->"http://identifiers.org/kegg.reaction/R02732", PFK26->"http://identifiers.org/uniprot/Q16877", PFK26->"http://identifiers.org/uniprot/Q16875", PFK26->"http://identifiers.org/uniprot/P16118", PFK26->"http://identifiers.org/uniprot/O60825", PGI->"http://identifiers.org/SBO:0000176", PGI->"http://identifiers.org/vmhreaction/PGI", PGI->"http://identifiers.org/metanetx.reaction/MNXR102535", PGI->"http://identifiers.org/seed/rxn00558", PGI->"http://identifiers.org/bigg.reaction/PGI", PGI->"http://identifiers.org/biocyc/META:PGLUCISOM-RXN", PGI->"http://identifiers.org/ec-code/5.3.1.9", PGI->"http://identifiers.org/uniprot/P06744", PGK->"http://identifiers.org/SBO:0000176", PGK->"http://identifiers.org/vmhreaction/PGK", PGK->"http://identifiers.org/rhea/14801", PGK->"http://identifiers.org/rhea/14804", PGK->"http://identifiers.org/metanetx.reaction/MNXR102538", PGK->"http://identifiers.org/seed/rxn01100", PGK->"http://identifiers.org/bigg.reaction/PGK", PGK->"http://identifiers.org/biocyc/META:PHOSGLYPHOS-RXN", PGK->"http://identifiers.org/ec-code/2.7.2.3", PGK->"http://identifiers.org/kegg.reaction/R01512", PGK->"http://identifiers.org/uniprot/P00558", PGK->"http://identifiers.org/uniprot/P07205", PGM->"http://identifiers.org/SBO:0000176", PGM->"http://identifiers.org/vmhreaction/PGM", PGM->"http://identifiers.org/rhea/15901", PGM->"http://identifiers.org/rhea/15904", PGM->"http://identifiers.org/metanetx.reaction/MNXR102547", PGM->"http://identifiers.org/seed/rxn01106", PGM->"http://identifiers.org/bigg.reaction/PGM", PGM->"http://identifiers.org/biocyc/META:3PGAREARR-RXN", PGM->"http://identifiers.org/ec-code/5.4.2.1", PGM->"http://identifiers.org/kegg.reaction/R01518", PGM->"http://identifiers.org/uniprot/P15259", PGM->"http://identifiers.org/uniprot/P07738", PGM->"http://identifiers.org/uniprot/P18669", PYK->"http://identifiers.org/SBO:0000176", PYK->"http://identifiers.org/vmhreaction/PYK", PYK->"http://identifiers.org/rhea/18160", PYK->"http://identifiers.org/rhea/18157", PYK->"http://identifiers.org/metanetx.reaction/MNXR103371", PYK->"http://identifiers.org/seed/rxn00148", PYK->"http://identifiers.org/bigg.reaction/PYK", PYK->"http://identifiers.org/biocyc/META:PEPDEPHOS-RXN", PYK->"http://identifiers.org/ec-code/2.7.1.40", PYK->"http://identifiers.org/kegg.reaction/R00200", PYK->"http://identifiers.org/uniprot/P14618", PYK->"http://identifiers.org/uniprot/P30613", PYRt2r->"http://identifiers.org/SBO:0000655", PYRt2r->"http://identifiers.org/vmhreaction/PYRt2r", PYRt2r->"http://identifiers.org/bigg.reaction/PYRt2", PYRt2r->"http://identifiers.org/metanetx.reaction/MNXR103385", TPI->"http://identifiers.org/SBO:0000176", TPI->"http://identifiers.org/vmhreaction/TPI", TPI->"http://identifiers.org/metanetx.reaction/MNXR104918", TPI->"http://identifiers.org/seed/rxn00747", TPI->"http://identifiers.org/bigg.reaction/TPI", TPI->"http://identifiers.org/biocyc/META:TRIOSEPISOMERIZATION-RXN", TPI->"http://identifiers.org/ec-code/5.3.1.1", TPI->"http://identifiers.org/kegg.reaction/R01015", TPI->"http://identifiers.org/rhea/18585", TPI->"http://identifiers.org/uniprot/P60174" }; units = { {"time" -> "min", "metabolite" -> "", "extent" -> ""} }; (* Time evolution *) odes = { adp'[t] == 1.0*ATPconsumption +1.0*HEX1 +1.0*PFK +1.0*PFK26 -1.0*ATPformation -1.0*PGK -1.0*PYK, atp'[t] == 1.0*ATPformation +1.0*PGK +1.0*PYK +1.0*ATP\[LetterSpace]ADP\[LetterSpace]CHANGE -1.0*ATPconsumption -1.0*HEX1 -1.0*PFK -1.0*PFK26, bpg13'[t] == 1.0*GAPD -1.0*PGK, dhap'[t] == 1.0*FBA -1.0*TPI, f26bp'[t] == 1.0*PFK26 -1.0*FBP26, f6p'[t] == 1.0*PGI +1.0*FBP +1.0*FBP26 -1.0*PFK -1.0*PFK26, fbp'[t] == 1.0*PFK -1.0*FBP -1.0*FBA, g6p'[t] == 1.0*HEX1 -1.0*PGI, glc'[t] == 1.0*GLCt1r -1.0*HEX1, grap'[t] == 1.0*FBA +1.0*TPI -1.0*GAPD, lac'[t] == 1.0*LDH\[LetterSpace]L -1.0*L\[LetterSpace]LACt2r, pep'[t] == 1.0*ENO -1.0*PYK, pg2'[t] == 1.0*PGM -1.0*ENO, pg3'[t] == 1.0*PGK -1.0*PGM, pyr'[t] == 1.0*PYK -1.0*PYRt2r -1.0*LDH\[LetterSpace]L }; timeCourse = NDSolve[Join[odes, initialValues]//.rateEquations//.assignments//.parameters, variables, {t, 0, 100}]; (* Steady-state solution initialized with result of time evolution *) findRootEquations = odes /.D[_[t],t]->0; findRootVariables = Partition[Flatten[{#, #/.timeCourse/.t->100} &/@variables],2]; steadyStateVariables = FindRoot[findRootEquations//.rateEquations//.assignments//.parameters, findRootVariables, MaxIterations->100] fluxes = #//.assignments//.parameters/.steadyStateVariables&/@rateEquations (* Plot the time evolution of the variables *) plotTable=Table[Plot[variables[[i]]/.parameters/.timeCourse,{t,0,100},PlotLegends->variables[[i]],PlotRange->Full],{i,Length[variables]}]