#levchenko2



#reactions
Reaction1:
 {1.0}RAF + {1.0}RAFK = {1.0}RAFRAFK
  a1 * RAF * RAFK
Reaction10:
 {1.0}MEKp + {1.0}MEKPH = {1.0}MEKpMEKPH
  a4 * MEKp * MEKPH
Reaction11:
 {1.0}MEKpMEKPH = {1.0}MEKp + {1.0}MEKPH
  d4 * MEKpMEKPH
Reaction12:
 {1.0}MEKpMEKPH = {1.0}MEK + {1.0}MEKPH
  k4 * MEKpMEKPH
Reaction13:
 {1.0}MEKp + {1.0}RAFp = {1.0}MEKpRAFp
  a5 * MEKp * RAFp
Reaction14:
 {1.0}MEKpRAFp = {1.0}MEKp + {1.0}RAFp
  d5 * MEKpRAFp
Reaction15:
 {1.0}MEKpRAFp = {1.0}MEKpp + {1.0}RAFp
  k5 * MEKpRAFp
Reaction16:
 {1.0}MEKPH + {1.0}MEKpp = {1.0}MEKppMEKPH
  a6 * MEKPH * MEKpp
Reaction17:
 {1.0}MEKppMEKPH = {1.0}MEKPH + {1.0}MEKpp
  d6 * MEKppMEKPH
Reaction18:
 {1.0}MEKppMEKPH = {1.0}MEKp + {1.0}MEKPH
  k6 * MEKppMEKPH
Reaction19:
 {1.0}MAPK + {1.0}MEKpp = {1.0}MAPKMEKpp
  a7 * MAPK * MEKpp
Reaction2:
 {1.0}RAFRAFK = {1.0}RAF + {1.0}RAFK
  d1 * RAFRAFK
Reaction20:
 {1.0}MAPKMEKpp = {1.0}MAPK + {1.0}MEKpp
  d7 * MAPKMEKpp
Reaction21:
 {1.0}MAPKMEKpp = {1.0}MAPKp + {1.0}MEKpp
  k7 * MAPKMEKpp
Reaction22:
 {1.0}MAPKp + {1.0}MAPKPH = {1.0}MAPKpMAPKPH
  a8 * MAPKp * MAPKPH
Reaction23:
 {1.0}MAPKpMAPKPH = {1.0}MAPKp + {1.0}MAPKPH
  d8 * MAPKpMAPKPH
Reaction24:
 {1.0}MAPKpMAPKPH = {1.0}MAPK + {1.0}MAPKPH
  k8 * MAPKpMAPKPH
Reaction25:
 {1.0}MAPKp + {1.0}MEKpp = {1.0}MAPKpMEKpp
  a9 * MAPKp * MEKpp
Reaction26:
 {1.0}MAPKpMEKpp = {1.0}MAPKp + {1.0}MEKpp
  d9 * MAPKpMEKpp
Reaction27:
 {1.0}MAPKpMEKpp = {1.0}MAPKpp + {1.0}MEKpp
  k9 * MAPKpMEKpp
Reaction28:
 {1.0}MAPKPH + {1.0}MAPKpp = {1.0}MAPKppMAPKPH
  a10 * MAPKPH * MAPKpp
Reaction29:
 {1.0}MAPKppMAPKPH = {1.0}MAPKPH + {1.0}MAPKpp
  d10 * MAPKppMAPKPH
Reaction3:
 {1.0}RAFRAFK = {1.0}RAFK + {1.0}RAFp
  k1 * RAFRAFK
Reaction30:
 {1.0}MAPKppMAPKPH = {1.0}MAPKp + {1.0}MAPKPH
  k10 * MAPKppMAPKPH
Reaction4:
 {1.0}RAFp + {1.0}RAFPH = {1.0}RAFpRAFPH
  a2 * RAFp * RAFPH
Reaction5:
 {1.0}RAFpRAFPH = {1.0}RAFp + {1.0}RAFPH
  d2 * RAFpRAFPH
Reaction6:
 {1.0}RAFpRAFPH = {1.0}RAF + {1.0}RAFPH
  k2 * RAFpRAFPH
Reaction7:
 {1.0}MEK + {1.0}RAFp = {1.0}MEKRAFp
  a3 * MEK * RAFp
Reaction8:
 {1.0}MEKRAFp = {1.0}MEK + {1.0}RAFp
  d3 * MEKRAFp
Reaction9:
 {1.0}MEKRAFp = {1.0}MEKp + {1.0}RAFp
  k3 * MEKRAFp
v_ENZ_C1_NEG:
 {1.0}C1 = $pool
  C1 * (on1 * MEK + on2 * MAPK)
v_ENZ_C1_POS:
 $pool = {1.0}C1
  of1 * C2 + of2 * C4 + of3 * C3 + of4 * C5
v_ENZ_C2_NEG:
 {1.0}C2 = $pool
  (of1 + on2 * MAPK) * C2 + kr1 * C2 * RAFp
v_ENZ_C2_POS:
 $pool = {1.0}C2
  on1 * C1 * MEK + of2 * C6 + of4 * C9
v_ENZ_C3_NEG:
 {1.0}C3 = $pool
  on2 * C3 * MAPK + of3 * C3
v_ENZ_C3_POS:
 $pool = {1.0}C3
  of2 * C7 + of4 * C8 + kr1 * C2 * RAFp
v_ENZ_C4_NEG:
 {1.0}C4 = $pool
  (of2 + on1 * MEK) * C4
v_ENZ_C4_POS:
 $pool = {1.0}C4
  of1 * C6 + on2 * MAPK * C1 + of3 * C7
v_ENZ_C5_NEG:
 {1.0}C5 = $pool
  (on1 * MEK + of4) * C5
v_ENZ_C5_POS:
 $pool = {1.0}C5
  of1 * C9 + of3 * C8
v_ENZ_C6_NEG:
 {1.0}C6 = $pool
  (of1 + of2) * C6 + kr1 * C6 * RAFp
v_ENZ_C6_POS:
 $pool = {1.0}C6
  on1 * MEK * C4 + on2 * MAPK * C2
v_ENZ_C7_NEG:
 {1.0}C7 = $pool
  of3 * C7 + kr2 * C7 + of2 * C7
v_ENZ_C7_POS:
 $pool = {1.0}C7
  on2 * MAPK * C3 + kr1 * C6 * RAFp
v_ENZ_C8_NEG:
 {1.0}C8 = $pool
  (of3 + of4) * C8
v_ENZ_C8_POS:
 $pool = {1.0}C8
  kr2 * C7 + kr1 * RAFp * C9
v_ENZ_C9_NEG:
 {1.0}C9 = $pool
  (of1 + of4) * C9 + kr1 * RAFp * C9
v_ENZ_C9_POS:
 $pool = {1.0}C9
  on1 * MEK * C5
v_ENZ_SCAF_MAPKPP_POS:
 $pool = {1.0}MAPKpp
  of4 * (C5 + C8 + C9)
v_ENZ_SCAF_MAPK_NEG:
 {1.0}MAPK = $pool
  on2 * MAPK * (C1 + C2 + C3)
v_ENZ_SCAF_MAPK_POS:
 $pool = {1.0}MAPK
  of2 * (C4 + C6 + C7)
v_ENZ_SCAF_MEKPP_POS:
 $pool = {1.0}MEKpp
  of3 * (C3 + C7 + C8)
v_ENZ_SCAF_MEK_NEG:
 {1.0}MEK = $pool
  on1 * MEK * (C1 + C4 + C5)
v_ENZ_SCAF_MEK_POS:
 $pool = {1.0}MEK
  of1 * (C2 + C6 + C9)


#parameters
initial_value_C1_Fraction = 1.0
initial_value_C2_Fraction = 0.0
initial_value_C4_Fraction = 0.0
initial_value_C6_Fraction = 0.0
initial_value_MAPK = 0.4
initial_value_MEK = 0.2
initial_value_RAFK = 0.2
initial_value_Total_Scaffold = 0.0
kr1 = 0.1
kr2 = 0.1
of1 = 0.05
of2 = 0.05
of3 = 0.05
of4 = 0.5
on1 = 10.0
on2 = 10.0
Reaction1_a1 = 1.0
Reaction10_a4 = 10.0
Reaction11_d4 = 0.8
Reaction12_k4 = 0.1
Reaction13_a5 = 3.3
Reaction14_d5 = 0.4
Reaction15_k5 = 0.1
Reaction16_a6 = 10.0
Reaction17_d6 = 0.8
Reaction18_k6 = 0.1
Reaction19_a7 = 20.0
Reaction2_d1 = 0.4
Reaction20_d7 = 0.6
Reaction21_k7 = 0.1
Reaction22_a8 = 5.0
Reaction23_d8 = 0.4
Reaction24_k8 = 0.1
Reaction25_a9 = 20.0
Reaction26_d9 = 0.6
Reaction27_k9 = 0.1
Reaction28_a10 = 5.0
Reaction29_d10 = 0.4
Reaction3_k1 = 0.1
Reaction30_k10 = 0.1
Reaction4_a2 = 0.5
Reaction5_d2 = 0.5
Reaction6_k2 = 0.1
Reaction7_a3 = 3.3
Reaction8_d3 = 0.42
Reaction9_k3 = 0.1
Cytoplasm = 1.0


#initial values
C1 = 0
C2 = 0
C3 = 0.0
C4 = 0
C5 = 0.0
C6 = 0
C7 = 0.0
C8 = 0.0
C9 = 0.0
MAPK = 0.4
MAPKMEKpp = 0.0
MAPKPH = 0.3
MAPKp = 0.0
MAPKpMAPKPH = 0.0
MAPKpMEKpp = 0.0
MAPKpp = 0.0
MAPKppMAPKPH = 0.0
MEK = 0.2
MEKPH = 0.2
MEKRAFp = 0.0
MEKp = 0.0
MEKpMEKPH = 0.0
MEKpRAFp = 0.0
MEKpp = 0.0
MEKppMEKPH = 0.0
RAF = 0.3
RAFK = 0.2
RAFPH = 0.3
RAFRAFK = 0.0
RAFp = 0.0
RAFpRAFPH = 0.0


#assignment rules