begin name goldbeter3 end name begin reactions reaction1 {1.0}$EXTERNAL = {1.0}C reaction2 {1.0}C = {1.0}$EXTERNAL reaction3 {1.0}C = {1.0}$EXTERNAL reaction4 {1.0}MI = {1.0}M reaction5 {1.0}M = {1.0}MI reaction6 {1.0}XI = {1.0}X reaction7 {1.0}X = {1.0}XI end reactions begin rate equations reaction1 = cell*reaction1vi reaction2 = cell*reaction2kd*C[t] reaction3 = (cell*reaction3vd*C[t]*X[t])/(reaction3Kd + C[t]) reaction4 = (cell*V1*MI[t])/(reaction4K1 + MI[t]) reaction5 = (cell*reaction5V2*M[t])/(reaction5K2 + M[t]) reaction6 = (cell*V3*XI[t])/(reaction6K3 + XI[t]) reaction7 = (cell*reaction7V4*X[t])/(reaction7K4 + X[t]) end rate equations begin parameters Kc = 0.5 VM1 = 3.0 VM3 = 1.0 reaction3vd = 0.25 reaction1vi = 0.025 reaction2kd = 0.01 reaction3Kd = 0.02 reaction4K1 = 0.005 reaction5V2 = 1.5 reaction5K2 = 0.005 reaction6K3 = 0.005 reaction7K4 = 0.005 reaction7V4 = 0.5 cell = 1.0 end parameters begin initial conditions C[0] = Ci M[0] = Mi MI[0] = MIi X[0] = Xi XI[0] = XIi end initial conditions begin initial values Ci = 0.01 Mi = 0.01 MIi = 0.99 Xi = 0.01 XIi = 0.99 end initial values begin assignment rules V3 := VM3*M[t] V1 := (VM1*C[t])/(Kc + C[t]) end assignment rules begin function definitions end function definitions begin events end events begin process annotations end process annotations begin species annotations end species annotations begin units end units