begin name
fisher2
end name

begin reactions
R1 {1.0}NFATPiNuc + {1.0}ActCNuc = {1.0}ActCNuc + {1.0}NFATNuc
R10 {1.0}NFATPiCyt = {1.0}NFATPiNuc
R11 {1.0}NFATActCCyt = {1.0}ActCCyt + {1.0}NFATCyt
R12 {1.0}NFATPiCyt + {1.0}ActCCyt = {1.0}ActCCyt + {1.0}NFATCyt
R13 {3.0}$CaCyt + {1.0}InactCCyt = {1.0}ActCCyt
R14 {3.0}$CaNuc + {1.0}InactCNuc = {1.0}ActCNuc
R15 {1.0}InactCCyt = {1.0}InactCNuc
R16 {1.0}$CaCyt = {1.0}$CaNuc
R17 {1.0}NFATPiActCCyt = {1.0}NFATPiActCNuc
R2 {1.0}ActCNuc + {1.0}NFATNuc = {1.0}NFATActCNuc
R3 {1.0}NFATNuc = {1.0}NFATCyt
R4 {1.0}ActCNuc = {1.0}ActCCyt
R5 {1.0}NFATActCNuc = {1.0}NFATPiActCNuc
R6 {1.0}NFATPiActCNuc = {1.0}ActCNuc + {1.0}NFATPiNuc
R7 {1.0}NFATActCNuc = {1.0}NFATActCCyt
R8 {1.0}NFATActCCyt = {1.0}NFATPiActCCyt
R9 {1.0}NFATPiActCCyt = {1.0}ActCCyt + {1.0}NFATPiCyt
end reactions

begin rate equations
R1 = nucleus*(-(k2*NFATNuc[t]) + k1*NFATPiNuc[t])
R10 = cytosol*k3*NFATPiCyt[t] - k4*nucleus*NFATPiNuc[t]
R11 = cytosol*(k15*NFATActCCyt[t] - k16*ActCCyt[t]*NFATCyt[t])
R12 = cytosol*(-(k2*NFATCyt[t]) + k1*NFATPiCyt[t])
R13 = cytosol*(-(k20*ActCCyt[t]) + CaCyt^3*k19*InactCCyt[t])
R14 = nucleus*(-(k20*ActCNuc[t]) + CaNuc^3*k19*InactCNuc[t])
R15 = cytosol*k5*InactCCyt[t] - k6*nucleus*InactCNuc[t]
R16 = CaCyt*cytosol*k21 - CaNuc*k22*nucleus
R17 = cytosol*k7*NFATPiActCCyt[t] - k8*nucleus*NFATPiActCNuc[t]
R2 = nucleus*(-(k15*NFATActCNuc[t]) + k16*ActCNuc[t]*NFATNuc[t])
R3 = -(cytosol*k17*NFATCyt[t]) + k18*nucleus*NFATNuc[t]
R4 = -(cytosol*k5*ActCCyt[t]) + k6*nucleus*ActCNuc[t]
R5 = nucleus*(k14*NFATActCNuc[t] - k13*NFATPiActCNuc[t])
R6 = nucleus*(k12*NFATPiActCNuc[t] - k11*ActCNuc[t]*NFATPiNuc[t])
R7 = -(cytosol*k9*NFATActCCyt[t]) + k10*nucleus*NFATActCNuc[t]
R8 = cytosol*(k14*NFATActCCyt[t] - k13*NFATPiActCCyt[t])
R9 = cytosol*(k12*NFATPiActCCyt[t] - k11*ActCCyt[t]*NFATPiCyt[t])
end rate equations

begin parameters
TimeinSeconds = 100.0
k1 = "2.56e-05"
k10 = 0.005
k11 = 6.63
k12 = 0.00168
k13 = 0.5
k14 = 0.00256
k15 = 0.00168
k16 = 6.63
k17 = 0.0015
k18 = 0.00096
k19 = 1.0
k2 = 0.00256
k20 = 1.0
k21 = 0.21
k22 = 0.5
k3 = 0.005
k4 = 0.5
k5 = 0.0019
k6 = 0.00092
k7 = 0.005
k8 = 0.5
k9 = 0.5
CaCyt = 1.0
CaNuc = 1.0
cytosol = "2.69e-13"
nucleus = "1.13e-13"
end parameters

begin initial conditions
ActCCyt[0] = ActCCyti
ActCNuc[0] = ActCNuci
InactCCyt[0] = InactCCyti
InactCNuc[0] = InactCNuci
NFATActCCyt[0] = NFATActCCyti
NFATActCNuc[0] = NFATActCNuci
NFATCyt[0] = NFATCyti
NFATNuc[0] = NFATNuci
NFATPiActCCyt[0] = NFATPiActCCyti
NFATPiActCNuc[0] = NFATPiActCNuci
NFATPiCyt[0] = NFATPiCyti
NFATPiNuc[0] = NFATPiNuci
end initial conditions

begin initial values
ActCCyti = 9.1e-06
ActCNuci = 5.05e-05
InactCCyti = 0.0097108
InactCNuci = 0.049198
NFATActCCyti = 6.1e-06
NFATActCNuci = 0.0009477
NFATCyti = 0.0001101
NFATNuci = 0.0005219
NFATPiActCCyti = 2.2e-06
NFATPiActCNuci = 2.5e-06
NFATPiCyti = 0.0094397
NFATPiNuci = 0.0002272
end initial values

begin assignment rules
end assignment rules

begin function definitions
end function definitions

begin events
event_0000001 = (-t + TimeinSeconds <= 0 && t < 1500, {CaCyt -> 1, CaNuc -> 1, TimeinSeconds -> 100 + TimeinSeconds}, 0)
event_0000003 = (t >= 1500, {CaCyt -> 0.1, CaNuc -> 0.1}, 0)
event_0000002 = (-t + TimeinSeconds <= 90 && t < 1500, {CaCyt -> 0.1, CaNuc -> 0.1}, 0)
end events

begin process annotations
R1 http://identifiers.org/ec-code/3.1.3.16
R1 http://identifiers.org/go/GO:0004722
R1 http://identifiers.org/go/GO:0004723
R1 http://identifiers.org/kegg.reaction/R00164
R12 http://identifiers.org/ec-code/3.1.3.16
R12 http://identifiers.org/go/GO:0004723
R3 http://identifiers.org/go/GO:0051531
end process annotations

begin species annotations
Ca_Cyt http://identifiers.org/chebi/CHEBI:29108
Ca_Cyt http://identifiers.org/kegg.compound/C00076
Ca_Nuc http://identifiers.org/chebi/CHEBI:29108
Ca_Nuc http://identifiers.org/kegg.compound/C00076
end species annotations

begin units
end units