#dallepezze2



#reactions
reaction_1:
 {1.0}Akt = {1.0}Akt_pS473
  Cell * function_4_reaction_1_1(Akt, Akt_S473_phos_by_insulin, Insulin)
reaction_10:
 {1.0}Mitophagy = {1.0}Nil
  Cell * function_4_reaction_10_1(Mitophagy, mTORC1_pS2448, mitophagy_inactiv_by_mTORC1_pS2448)
reaction_11:
 {1.0}FoxO3a = {1.0}FoxO3a_pS253
  Cell * function_4_reaction_11_1(Akt_pS473, FoxO3a, FoxO3a_phos_by_Akt_pS473)
reaction_12:
 {1.0}FoxO3a_pS253 = {1.0}FoxO3a
  Cell * function_4_reaction_12_1(FoxO3a_pS253, FoxO3a_phos_by_JNK_pT183, JNK_pT183)
reaction_13:
 {1.0}FoxO3a_pS253 = {1.0}Nil
  Cell * FoxO3a_pS253_degrad * FoxO3a_pS253
reaction_14:
 $pool = {1.0}FoxO3a
  Cell * function_2(FoxO3a_synthesis)
reaction_15:
 $pool = {1.0}CDKN1A
  Cell * function_4_reaction_15_1(CDKN1A_transcr_by_FoxO3a_n_DNA_damage, DNA_damage, FoxO3a)
reaction_16:
 {1.0}CDKN1A = {1.0}Nil
  Cell * function_4_reaction_16_1(Akt_pS473, CDKN1A, CDKN1A_inactiv_by_Akt_pS473)
reaction_17:
 $pool = {1.0}CDKN1B
  Cell * function_4_reaction_17_1(CDKN1B_transcr_by_FoxO3a_n_DNA_damage, DNA_damage, FoxO3a)
reaction_18:
 {1.0}CDKN1B = {1.0}Nil
  Cell * function_4_reaction_18_1(Akt_pS473, CDKN1B, CDKN1B_inactiv_by_Akt_pS473)
reaction_19:
 $pool = {1.0}DNA_damage
  Cell * function_4_reaction_19_1(DNA_damaged_by_irradiation, Irradiation)
reaction_2:
 {1.0}Akt_pS473 = {1.0}Akt
  Cell * function_4_reaction_2_1(Akt_pS473, Akt_pS473_dephos_by_mTORC1_pS2448, mTORC1_pS2448)
reaction_20:
 $pool = {1.0}DNA_damage
  Cell * function_4_reaction_20_1(DNA_damaged_by_ROS, ROS)
reaction_21:
 {1.0}DNA_damage = {1.0}Nil
  Cell * DNA_repair * DNA_damage
reaction_22:
 $pool = {1.0}ROS
  Cell * function_4_reaction_22_1(Mito_membr_pot_new, ROS_prod_by_Mito_membr_pot_new)
reaction_23:
 $pool = {1.0}ROS
  Cell * function_4_reaction_23_1(Mito_membr_pot_old, ROS_prod_by_Mito_membr_pot_old)
reaction_24:
 {1.0}ROS = {1.0}Nil
  Cell * ROS_turnover * ROS
reaction_25:
 {1.0}JNK = {1.0}JNK_pT183
  Cell * function_4_reaction_25_1(JNK, JNK_activ_by_ROS, ROS)
reaction_26:
 {1.0}JNK_pT183 = {1.0}JNK
  Cell * JNK_pT183_inactiv * JNK_pT183
reaction_27:
 $pool = {1.0}SA_beta_gal
  Cell * function_4_reaction_27_1(ROS, sen_ass_beta_gal_inc_by_ROS)
reaction_28:
 $pool = {1.0}SA_beta_gal
  Cell * function_4_reaction_28_1(Mitophagy, sen_ass_beta_gal_inc_by_Mitophagy)
reaction_29:
 {1.0}SA_beta_gal = $pool
  Cell * sen_ass_beta_gal_dec * SA_beta_gal
reaction_3:
 {1.0}AMPK = {1.0}AMPK_pT172
  Cell * AMPK_T172_phos * AMPK
reaction_30:
 {1.0}Mito_mass_turnover = {1.0}Mito_mass_new
  Cell * function_4_reaction_30_1(Mito_mass_turnover, mTORC1_pS2448, mito_biogenesis_by_mTORC1_pS2448)
reaction_31:
 {1.0}Mito_mass_turnover = {1.0}Mito_mass_new
  Cell * function_4_reaction_31_1(Mito_mass_turnover, mTORC1_pS2448, mito_biogenesis_by_AMPK_pT172)
reaction_32:
 {1.0}Mito_mass_new = {1.0}Mito_mass_turnover
  Cell * function_4_reaction_32_1(Mito_mass_new, Mitophagy, mitophagy_new)
reaction_33:
 {1.0}Mito_mass_old = {1.0}Mito_mass_turnover
  Cell * function_4_reaction_33_1(Mito_mass_old, Mitophagy, mitophagy_old)
reaction_34:
 {1.0}Mito_mass_new = {1.0}Mito_mass_old
  Cell * function_4_reaction_34_1(CDKN1A, Mito_mass_new, mito_dysfunction)
reaction_35:
 $pool = {1.0}Mito_membr_pot_new
  Cell * function_4_reaction_35_1(Mito_mass_new, mito_membr_pot_new_inc)
reaction_36:
 $pool = {1.0}Mito_membr_pot_old
  Cell * function_4_reaction_36_1(Mito_mass_old, mito_membr_pot_old_inc)
reaction_37:
 {1.0}Mito_membr_pot_new = {1.0}Nil
  Cell * mito_membr_pot_new_dec * Mito_membr_pot_new
reaction_38:
 {1.0}Mito_membr_pot_old = {1.0}Nil
  Cell * mito_membr_pot_old_dec * Mito_membr_pot_old
reaction_39:
 $pool = {1.0}IKKbeta
  Cell * function_4_reaction_39_1(IKKbeta_activ_by_ROS, ROS)
reaction_4:
 {1.0}AMPK_pT172 = {1.0}AMPK
  Cell * function_4_reaction_4_1(AMPK_pT172, AMPK_pT172_dephos_by_Mito_membr_pot_new, Mito_membr_pot_new)
reaction_40:
 {1.0}IKKbeta = {1.0}Nil
  Cell * IKKbeta_inactiv * IKKbeta
reaction_41:
 {1.0}mTORC1 = {1.0}mTORC1_pS2448
  Cell * function_4_reaction_41_1(Amino_Acids, IKKbeta, mTORC1, mTORC1_S2448_phos_by_AA_n_IKKbeta)
reaction_5:
 {1.0}AMPK_pT172 = {1.0}AMPK
  Cell * function_4_reaction_5_1(AMPK_pT172, AMPK_pT172_dephos_by_Mito_membr_pot_old, Mito_membr_pot_old)
reaction_6:
 {1.0}mTORC1 = {1.0}mTORC1_pS2448
  Cell * function_4_reaction_6_1(Amino_Acids, mTORC1, mTORC1_S2448_phos_by_AA)
reaction_7:
 {1.0}mTORC1 = {1.0}mTORC1_pS2448
  Cell * function_4_reaction_7_1(Akt_pS473, Amino_Acids, mTORC1, mTORC1_S2448_phos_by_AA_n_Akt_pS473)
reaction_8:
 {1.0}mTORC1_pS2448 = {1.0}mTORC1
  Cell * function_4_reaction_8_1(AMPK_pT172, mTORC1_pS2448, mTORC1_pS2448_dephos_by_AMPK_pT172)
reaction_9:
 $pool = {1.0}Mitophagy
  Cell * function_4_reaction_9_1(AMPK_pT172, FoxO3a, mitophagy_activ_by_FoxO3a_n_AMPK_pT172)


#parameters
AMPK_T172_phos = 0.355183987378767
AMPK_pT172_dephos_by_Mito_membr_pot_new = 0.117744691539618
AMPK_pT172_dephos_by_Mito_membr_pot_old = 1.00000000000003e-06
Akt_S473_phos_by_insulin = 0.588783148144923
Akt_pS473_dephos_by_mTORC1_pS2448 = 0.114598191621279
CDKN1A_inactiv_by_Akt_pS473 = 0.0667971061916905
CDKN1A_transcr_by_FoxO3a_n_DNA_damage = 0.0852182335681166
CDKN1B_inactiv_by_Akt_pS473 = 0.0596841598127919
CDKN1B_transcr_by_FoxO3a_n_DNA_damage = 0.0920526565951487
DNA_damaged_by_ROS = 0.118873655169353
DNA_damaged_by_irradiation = 9237.72311545872
DNA_repair = 0.325724769122274
FoxO3a_pS253_degrad = 39.4068609318082
FoxO3a_phos_by_Akt_pS473 = 6.83511123229576
FoxO3a_phos_by_JNK_pT183 = 0.112877630496044
FoxO3a_synthesis = 407.307409980937
IKKbeta_activ_by_ROS = 1.0
IKKbeta_inactiv = 1.0
JNK_activ_by_ROS = 0.00502329152478409
JNK_pT183_inactiv = 0.0718429173444438
ROS_prod_by_Mito_membr_pot_new = 4.55464788075885
ROS_prod_by_Mito_membr_pot_old = 772.829490967078
ROS_turnover = 3.23082321168464
mTORC1_S2448_phos_by_AA = 1.00008999860285e-06
mTORC1_S2448_phos_by_AA_n_Akt_pS473 = 162.471039450073
mTORC1_S2448_phos_by_AA_n_IKKbeta = 1.00008996727694e-05
mTORC1_pS2448_dephos_by_AMPK_pT172 = 191.297262771509
mito_biogenesis_by_AMPK_pT172 = 5.8915457309741e-05
mito_biogenesis_by_mTORC1_pS2448 = 0.0133620123598202
mito_dysfunction = 0.0270695257507146
mito_membr_pot_new_dec = 1094.58423149719
mito_membr_pot_new_inc = 9882.02736076158
mito_membr_pot_old_dec = 0.954903499913184
mito_membr_pot_old_inc = 0.00586017882122243
mitophagy_activ_by_FoxO3a_n_AMPK_pT172 = 1319.84219165251
mitophagy_inactiv_by_mTORC1_pS2448 = 645.999307230137
mitophagy_new = 0.22465992989378
mitophagy_old = 0.00122607614891116
scale_AMPK_pT172_obs = 1.0
scale_Akt_pS473_obs = 1.0
scale_CDKN1A_obs = 1.0
scale_CDKN1B_obs = 1.0
scale_DNA_damage_gammaH2AX_obs = 1.0
scale_FoxO3a_pS253_obs = 1.0
scale_FoxO3a_total_obs = 1.0
scale_JNK_pT183_obs = 1.0
scale_Mito_Mass_obs = 1.0
scale_Mito_Membr_Pot_obs = 1.0
scale_Mitophagy_obs = 1.0
scale_ROS_obs = 1.0
scale_SA_beta_gal_obs = 1.0
scale_mTOR_pS2448_obs = 1.0
sen_ass_beta_gal_dec = 0.154821166783837
sen_ass_beta_gal_inc_by_Mitophagy = 1.00000000000011e-06
sen_ass_beta_gal_inc_by_ROS = 0.0701139988718817
Cell = 1.0


#initial values
AMPK = 10.0
AMPK_pT172 = 10.0
Akt = 10.0
Akt_pS473 = 10.0
CDKN1A = 10.0
CDKN1B = 10.0
DNA_damage = 1.0
FoxO3a = 10.0
FoxO3a_pS253 = 10.0
IKKbeta = 10.0
JNK = 25.0
JNK_pT183 = 10.0
Mito_mass_new = 1.0
Mito_mass_old = 0.0
Mito_mass_turnover = 25.0
Mito_membr_pot_new = 12.12
Mito_membr_pot_old = 0.0
Mitophagy = 10.0
Nil = 0.0
ROS = 10.0
SA_beta_gal = 0.81
mTORC1 = 10.0
mTORC1_pS2448 = 10.0


#assignment rules
!F Akt_pS473_obs = scale_Akt_pS473_obs * Akt_pS473
!F Irradiation = piecewise(0.0, lt(time, -1.0), piecewise(0.0, lt(time, 0.0), piecewise(1.0, lt(time, 0.003472), 0.0)))
!F Amino_Acids = piecewise(1.0, lt(time, -1.0), piecewise(1.0, lt(time, 0.0), 1.0))
!F Insulin = piecewise(1.0, lt(time, -1.0), piecewise(1.0, lt(time, 0.0), 1.0))
!F DNA_damage_gammaH2AX_obs = scale_DNA_damage_gammaH2AX_obs * DNA_damage
!F mTOR_pS2448_obs = scale_mTOR_pS2448_obs * mTORC1_pS2448
!F FoxO3a_pS253_obs = scale_FoxO3a_pS253_obs * FoxO3a_pS253
!F AMPK_pT172_obs = scale_AMPK_pT172_obs * AMPK_pT172
!F Mito_Mass_obs = scale_Mito_Mass_obs * (Mito_mass_new + Mito_mass_old)
!F CDKN1A_obs = scale_CDKN1A_obs * CDKN1A
!F CDKN1B_obs = scale_CDKN1B_obs * CDKN1B
!F Mito_Membr_Pot_obs = scale_Mito_Membr_Pot_obs * (Mito_membr_pot_new + Mito_membr_pot_old)
!F Mitophagy_obs = scale_Mitophagy_obs * Mitophagy
!F FoxO3a_total_obs = scale_FoxO3a_total_obs * (FoxO3a + FoxO3a_pS253)
!F ROS_obs = scale_ROS_obs * ROS
!F JNK_pT183_obs = scale_JNK_pT183_obs * JNK_pT183
!F SA_beta_gal_obs = scale_SA_beta_gal_obs * SA_beta_gal