begin name
curien2
end name

begin reactions
Vak1 {1.0}$Asp = {1.0}AspP
Vak2 {1.0}$Asp = {1.0}AspP
VakI {1.0}$Asp = {1.0}AspP
VakII {1.0}$Asp = {1.0}AspP
Vasadh {1.0}AspP = {1.0}ASA
Vcgs {1.0}PHser = {1.0}$Cysta
Vdhdps1 {1.0}ASA = {1.0}Lys
Vdhdps2 {1.0}ASA = {1.0}Lys
Vhsdh1 {1.0}ASA = {1.0}Hser
Vhsdh2 {1.0}ASA = {1.0}Hser
Vhsk {1.0}Hser = {1.0}PHser
VileTRNA {1.0}Ile = {1.0}$IleTRNA
VlysKR {1.0}Lys = {1.0}$Sacc
VlysTRNA {1.0}Lys = {1.0}$LysTRNA
Vtd {1.0}Thr = {1.0}Ile
Vtha {1.0}Thr = {1.0}$Gly
VthrTRNA {1.0}Thr = {1.0}$ThrTRNA
Vts1 {1.0}PHser = {1.0}Thr
end reactions

begin rate equations
Vak1 = (AK1*c1*(Vak1AK1kforwardappexp - Vak1AK1kreverseappexp*AspP[t]))/(1 + (((1 + AdoMet/Vak1AK1AdoMetKaappexp)*Lys[t])/Vak1AK1LysKiappexp)^Vak1AK1nHexp)
Vak2 = (AK2*c1*(Vak2AK2kforwardappexp - Vak2AK2kreverseappexp*AspP[t]))/(1 + (Lys[t]/Vak2AK2LysKiappexp)^Vak2AK2nHexp)
VakI = (AKHSDHI*c1*(VakIAKIkforwardappexp - VakIAKIkreverseappexp*AspP[t]))/(1 + (Thr[t]/VakIAKIThrKiappexp)^VakIAKInHexp)
VakII = (AKHSDHII*c1*(VakIIAKIIkforwardappexp - VakIIAKIIkreverseappexp*AspP[t]))/(1 + (Thr[t]/VakIIAKIIThrKiappexp)^VakIIAKIInHexp)
Vasadh = ASADH*c1*(-(VasadhASADHkreverseappexp*ASA[t]) + VasadhASADHkforwardappexp*AspP[t])
Vcgs = (c1*CGS*VcgsCGSkcatexp*PHser[t])/((1 + VcgsCGSCysKmexp/Cys)*(((1 + Phosphate/VcgsCGSPhosphateKiexp)*VcgsCGSPhserKmexp)/(1 + VcgsCGSCysKmexp/Cys) + PHser[t]))
Vdhdps1 = (c1*DHDPS1*Vdhdps1DHDPS1kappexp*ASA[t])/(1 + (Lys[t]/Vdhdps1DHDPS1LysKiappexp)^Vdhdps1DHDPS1nHexp)
Vdhdps2 = (c1*DHDPS2*Vdhdps2DHDPS2kappexp*ASA[t])/(1 + (Lys[t]/Vdhdps2DHDPS2LysKiappexp)^Vdhdps2DHDPS2nHexp)
Vhsdh1 = AKHSDHI*c1*Vhsdh1HSDHIkforwardappexp*ASA[t]*(Vhsdh1HSDHIThrrelativeresidualactivityappexp + Vhsdh1HSDHIThrrelativeinhibitionappexp/(1 + Thr[t]/Vhsdh1HSDHIThrKiappexp))
Vhsdh2 = AKHSDHII*c1*Vhsdh2HSDHIIkforwardappexp*ASA[t]*(Vhsdh2HSDHIIThrrelativeresidualactivityappexp + Vhsdh2HSDHIIThrrelativeinhibitionappexp/(1 + Thr[t]/Vhsdh2HSDHIIThrKiappexp))
Vhsk = (c1*HSK*VhskHSKkcatappexp*Hser[t])/(VhskHSKHserappexp + Hser[t])
VileTRNA = (c1*VIleRS*Ile[t])/(VileTRNAIletRNASIleKm + Ile[t])
VlysKR = (c1*LKR*VlysKRLKRkcatexp*Lys[t])/(VlysKRLKRLysKmexp + Lys[t])
VlysTRNA = (c1*VLysRS*Lys[t])/(VlysTRNALystRNASLysKm + Lys[t])
Vtd = (c1*TD*VtdTDkappexp*Thr[t])/(1 + (Ile[t]/(VtdTDIleKinoValappexp + (Val*VtdTDValKa1appexp)/(Val + VtdTDValKa2appexp)))^VtdTDnHappexp)
Vtha = (c1*THA*VthaTHAkcatexp*Thr[t])/(VthaTHAThrKmexp + Thr[t])
VthrTRNA = (c1*VThrRS*Thr[t])/(VthrTRNAThrtRNASThrKm + Thr[t])
Vts1 = (c1*((AdoMet^Vts1TS1nHexp*Vts1TS1AdoMetkcatmaxexp)/Vts1TS1AdoMetKa1exp + Vts1TS1kcatminexp)*PHser[t]*TS1[t])/((1 + AdoMet^Vts1TS1nHexp/Vts1TS1AdoMetKa1exp)*(((1 + AdoMet/Vts1TS1AdoMetKa2exp)*Vts1TS1AdoMEtKmnoAdoMetexp*(1 + Phosphate/Vts1TS1PhosphateKiexp))/((1 + AdoMet/Vts1TS1AdoMetKa3exp)*(1 + AdoMet^Vts1TS1nHexp/Vts1TS1AdoMetKa4exp)) + PHser[t]))
end rate equations

begin parameters
VAARS = 0.43
AK1 = 0.25
AK2 = 0.25
AKHSDHI = 0.63
AKHSDHII = 0.63
ASADH = 11.6
AdoMet = 20.0
Asp = 1500.0
CGS = 0.7
Cys = 15.0
Cysta = 0.0
DHDPS1 = 1.6
DHDPS2 = 1.6
Gly = 0.0
HSK = 4.0
IleTRNA = 0.0
LKR = 0.0
LysTRNA = 0.0
Phosphate = 10000.0
Sacc = 0.0
TD = 0.36
THA = 0.0
ThrTRNA = 0.0
Val = 100.0
Vak1AK1kforwardappexp = 5.65
Vak1AK1kreverseappexp = 1.6
Vak1AK1LysKiappexp = 550.0
Vak1AK1AdoMetKaappexp = 3.5
Vak1AK1nHexp = 2.0
Vak2AK2kforwardappexp = 3.15
Vak2AK2kreverseappexp = 0.86
Vak2AK2LysKiappexp = 22.0
Vak2AK2nHexp = 1.1
VakIAKIkforwardappexp = 0.36
VakIAKIkreverseappexp = 0.15
VakIAKIThrKiappexp = 124.0
VakIAKInHexp = 2.0
VakIIAKIIkforwardappexp = 1.35
VakIIAKIIkreverseappexp = 0.22
VakIIAKIIThrKiappexp = 109.0
VakIIAKIInHexp = 2.0
VasadhASADHkforwardappexp = 0.9
VasadhASADHkreverseappexp = 0.23
Vdhdps1DHDPS1kappexp = 1.0
Vdhdps1DHDPS1LysKiappexp = 10.0
Vdhdps1DHDPS1nHexp = 2.0
Vdhdps2DHDPS2kappexp = 1.0
Vdhdps2DHDPS2LysKiappexp = 33.0
Vdhdps2DHDPS2nHexp = 2.0
Vhsdh1HSDHIkforwardappexp = 0.84
Vhsdh1HSDHIThrrelativeresidualactivityappexp = 0.14
Vhsdh1HSDHIThrrelativeinhibitionappexp = 0.86
Vhsdh1HSDHIThrKiappexp = 400.0
Vhsdh2HSDHIIkforwardappexp = 0.64
Vhsdh2HSDHIIThrrelativeresidualactivityappexp = 0.25
Vhsdh2HSDHIIThrrelativeinhibitionappexp = 0.75
Vhsdh2HSDHIIThrKiappexp = 8500.0
VhskHSKkcatappexp = 2.8
VhskHSKHserappexp = 14.0
Vts1TS1kcatminexp = 0.42
Vts1TS1AdoMetkcatmaxexp = 3.5
Vts1TS1AdoMetKa1exp = 73.0
Vts1TS1nHexp = 2.0
Vts1TS1PhosphateKiexp = 1000.0
Vts1TS1AdoMEtKmnoAdoMetexp = 250.0
Vts1TS1AdoMetKa2exp = 0.5
Vts1TS1AdoMetKa3exp = 1.09
Vts1TS1AdoMetKa4exp = 140.0
VcgsCGSkcatexp = 30.0
VcgsCGSCysKmexp = 460.0
VcgsCGSPhserKmexp = 2500.0
VcgsCGSPhosphateKiexp = 2000.0
VtdTDkappexp = 0.0124
VtdTDIleKinoValappexp = 30.0
VtdTDValKa1appexp = 73.0
VtdTDValKa2appexp = 615.0
VtdTDnHappexp = 3.0
VlysTRNALystRNASLysKm = 25.0
VthrTRNAThrtRNASThrKm = 100.0
VileTRNAIletRNASIleKm = 20.0
VlysKRLKRkcatexp = 3.1
VlysKRLKRLysKmexp = 13000.0
VthaTHAkcatexp = 1.7
VthaTHAThrKmexp = 7100.0
c1 = 1.0
end parameters

begin initial conditions
ASA[0] = ASAi
AspP[0] = AspPi
Hser[0] = Hseri
Ile[0] = Ilei
Lys[0] = Lysi
PHser[0] = PHseri
TS1[0] = TS1i
Thr[0] = Thri
end initial conditions

begin initial values
ASAi = 0.0
AspPi = 0.0
Hseri = 0.0
Ilei = 0.0
Lysi = 0.0
PHseri = 0.0
TS1i = 7.4
Thri = 0.0
end initial values

begin assignment rules
VIleRS := VAARS
VThrRS := VAARS
VLysRS := VAARS
end assignment rules

begin function definitions
end function definitions

begin events
end events

begin process annotations
VileTRNA http://identifiers.org/ec-code/6.1.1.5
VileTRNA http://identifiers.org/kegg.reaction/R03656
VileTRNA http://identifiers.org/obo.go/GO:0006428
VlysKR http://identifiers.org/ec-code/1.5.1.8
VlysKR http://identifiers.org/kegg.reaction/R00716
VlysKR http://identifiers.org/obo.go/GO:0047130
Vtha http://identifiers.org/ec-code/4.1.2.5
Vtha http://identifiers.org/kegg.reaction/R00751
Vtha http://identifiers.org/obo.go/GO:0004793
VthrTRNA http://identifiers.org/ec-code/6.1.1.3
VthrTRNA http://identifiers.org/kegg.reaction/R03663
VthrTRNA http://identifiers.org/obo.go/GO:0006435
VthrTRNA http://identifiers.org/obo.go/GO:0004812
end process annotations

begin species annotations
AK1 http://identifiers.org/uniprot/Q9LYU8
AK2 http://identifiers.org/uniprot/O23653
AKHSDHI http://identifiers.org/uniprot/Q9SA18
AKHSDHII http://identifiers.org/uniprot/O81852
ASA http://identifiers.org/obo.chebi/CHEBI:13086
ASA http://identifiers.org/kegg.compound/C00441
ASADH http://identifiers.org/uniprot/Q9FVC4
AdoMet http://identifiers.org/obo.chebi/CHEBI:15414
AdoMet http://identifiers.org/kegg.compound/C00019
Asp http://identifiers.org/obo.chebi/CHEBI:17053
Asp http://identifiers.org/kegg.compound/C00049
Asp http://identifiers.org/pubchem.substance/47205730
AspP http://identifiers.org/obo.chebi/CHEBI:15836
AspP http://identifiers.org/kegg.compound/C03082
CGS http://identifiers.org/uniprot/P55217
Cys http://identifiers.org/obo.chebi/CHEBI:17561
Cys http://identifiers.org/kegg.compound/C00097
Cysta http://identifiers.org/obo.chebi/CHEBI:17755
Cysta http://identifiers.org/kegg.compound/C00542
DHDPS1 http://identifiers.org/uniprot/Q9LZX6
DHDPS2 http://identifiers.org/uniprot/Q9FVC8
Gly http://identifiers.org/obo.chebi/CHEBI:15428
Gly http://identifiers.org/kegg.compound/C00037
HSK http://identifiers.org/uniprot/Q9XEE0
Hser http://identifiers.org/obo.chebi/CHEBI:15699
Hser http://identifiers.org/kegg.compound/C00263
Ile http://identifiers.org/obo.chebi/CHEBI:17191
Ile http://identifiers.org/kegg.compound/C00407
IleTRNA http://identifiers.org/obo.chebi/CHEBI:29160
IleTRNA http://identifiers.org/kegg.compound/C03127
LKR http://identifiers.org/uniprot/Q9SMZ4
Lys http://identifiers.org/obo.chebi/CHEBI:18019
Lys http://identifiers.org/kegg.compound/C00047
Lys http://identifiers.org/pubchem.substance/47205736
LysTRNA http://identifiers.org/obo.chebi/CHEBI:16047
LysTRNA http://identifiers.org/kegg.compound/C01931
PHser http://identifiers.org/obo.chebi/CHEBI:15961
PHser http://identifiers.org/kegg.compound/C01102
Phosphate http://identifiers.org/obo.chebi/CHEBI:18367
Phosphate http://identifiers.org/kegg.compound/C00009
Sacc http://identifiers.org/obo.chebi/CHEBI:16927
Sacc http://identifiers.org/kegg.compound/C00449
TD http://identifiers.org/uniprot/Q9ZSS6
TS1 http://identifiers.org/uniprot/Q9S7B5
Thr http://identifiers.org/obo.chebi/CHEBI:16857
Thr http://identifiers.org/kegg.compound/C00188
ThrTRNA http://identifiers.org/obo.chebi/CHEBI:29163
ThrTRNA http://identifiers.org/kegg.compound/C02992
Val http://identifiers.org/obo.chebi/CHEBI:16414
Val http://identifiers.org/kegg.compound/C00183
end species annotations

begin units
end units