begin name
calzone1
end name

begin reactions
Nuclei1 {1.0}$EXTERNAL = {1.0}N
R1 {1.0}$EXTERNAL = {1.0}MPFc
R10 {1.0}Stgm = {1.0}$EXTERNAL
R12 {1.0}$EXTERNAL = {1.0}Stgc
R13 {1.0}Stgc = {1.0}StgPc
R14 {1.0}StgPc = {1.0}Stgc
R15 {1.0}Stgc = {1.0}$EXTERNAL
R16 {1.0}StgPc = {1.0}$EXTERNAL
R19 {1.0}MPFc = {1.0}$EXTERNAL
R2 {1.0}preMPFc = {1.0}MPFc
R20 {1.0}preMPFc = {1.0}$EXTERNAL
R21 {1.0}Wee1Pn = {1.0}$EXTERNAL
R22 {1.0}$EXTERNAL = {1.0}$EXTERNAL
R23 {1.0}Wee1n = {1.0}$EXTERNAL
R24 {1.0}Wee1c = {1.0}$EXTERNAL
R25 {1.0}StgPn = {1.0}$EXTERNAL
R26 {1.0}StgPc = {1.0}$EXTERNAL
R27 {1.0}Stgn = {1.0}$EXTERNAL
R28 {1.0}Stgc = {1.0}$EXTERNAL
R29 {1.0}preMPFn = {1.0}MPFn
R3 {1.0}MPFc = {1.0}preMPFc
R30 {1.0}MPFn = {1.0}preMPFn
R33 {1.0}preMPFn = {1.0}$EXTERNAL
R34 {1.0}MPFn = {1.0}$EXTERNAL
R37 {1.0}IEa1 = {1.0}$EXTERNAL
R38 {1.0}$EXTERNAL = {1.0}IEa1
R39 {1.0}$EXTERNAL = {1.0}FZYa
R40 {1.0}FZYa = {1.0}$EXTERNAL
R41 {1.0}Wee1n = {1.0}Wee1Pn
R42 {1.0}Wee1Pn = {1.0}Wee1n
R43 {1.0}Stgn = {1.0}StgPn
R44 {1.0}StgPn = {1.0}Stgn
R45 {1.0}Stgn = {1.0}$EXTERNAL
R46 {1.0}StgPn = {1.0}$EXTERNAL
R6 {1.0}preMPFc = {1.0}$EXTERNAL
R7 {1.0}MPFc = {1.0}$EXTERNAL
R8 {1.0}Wee1c = {1.0}$EXTERNAL
R9 {1.0}$EXTERNAL = {1.0}Wee1c
16 {1.0}$EXTERNAL = {1.0}MPFn
18 {1.0}$EXTERNAL = {1.0}preMPFn
1821 {1.0}$EXTERNAL = {1.0}$EXTERNAL
20 {1.0}$EXTERNAL = {1.0}Wee1Pn
22 {1.0}$EXTERNAL = {1.0}Wee1c
24 {1.0}$EXTERNAL = {1.0}Wee1n
26 {1.0}$EXTERNAL = {1.0}StgPc
28 {1.0}$EXTERNAL = {1.0}StgPn
30 {1.0}$EXTERNAL = {1.0}Stgc
32 {1.0}$EXTERNAL = {1.0}Stgn
50 {1.0}$EXTERNAL = {1.0}Xm
51 {1.0}$EXTERNAL = {1.0}Xp
exportofMPFfromnucleus1 {1.0}MPFn = {1.0}$EXTERNAL
exportofpreMPFnfromnucleus1 {1.0}preMPFn = {1.0}$EXTERNAL
importofMPFintocytoplasm1 {1.0}$EXTERNAL = {1.0}MPFc
importofpreMPFintocytoplaslm1 {1.0}$EXTERNAL = {1.0}preMPFc
end reactions

begin rate equations
Nuclei1 = 0
R1 = cytoplasm*ksc
R10 = nuclei*((kdmp*Stgm[t])/(Jm + Stgm[t]) + kdm*Stgm[t]*Xp[t])
R12 = cytoplasm*ksstg*Stgm[t]
R13 = (cytoplasm*(kastgp + kastg*MPFc[t])*Stgc[t])/(Jastg + Stgc[t])
R14 = (cytoplasm*kistg*StgPc[t])/(Jistg + StgPc[t])
R15 = cytoplasm*kdstg*Stgc[t]
R16 = cytoplasm*kdstg*StgPc[t]
R19 = (cytoplasm*E1*kt*MPFc[t]*N[t])/(1 - E1*N[t])
R2 = cytoplasm*preMPFc[t]*(kstgp + kstg*StgPc[t])
R20 = (cytoplasm*E1*kt*N[t]*preMPFc[t])/(1 - E1*N[t])
R21 = koutw1*nuclei*Wee1Pn[t]
R22 = (cytoplasm*E1*kinw1*Wee1Pc*N[t])/(1 - E1*N[t])
R23 = koutw1*nuclei*Wee1n[t]
R24 = (cytoplasm*E1*kinw1*N[t]*Wee1c[t])/(1 - E1*N[t])
R25 = kouts1*nuclei*StgPn[t]
R26 = (cytoplasm*E1*kins1*N[t]*StgPc[t])/(1 - E1*N[t])
R27 = kouts1*nuclei*Stgn[t]
R28 = (cytoplasm*E1*kins1*N[t]*Stgc[t])/(1 - E1*N[t])
R29 = nuclei*preMPFn[t]*(kstgp + kstg*StgPn[t])
R3 = cytoplasm*MPFc[t]*(kweep + kwee*Wee1c[t])
R30 = nuclei*MPFn[t]*(kweep + kwee*Wee1n[t])
R33 = nuclei*(kdnp + kdn*FZYa[t])*preMPFn[t]
R34 = nuclei*(kdnp + kdn*FZYa[t])*MPFn[t]
R37 = (kiie*nuclei*IEa1[t])/(Jiie + IEa1[t])
R38 = (kaie*nuclei*(1 - IEa1[t])*MPFn[t])/(1 + Jaie - IEa1[t])
R39 = (kafzy*nuclei*(1 - FZYa[t])*IEa1[t])/(1 + Jafzy - FZYa[t])
R40 = (kifzy*nuclei*FZYa[t])/(Jifzy + FZYa[t])
R41 = (nuclei*(kiweep + kiwee*MPFn[t])*Wee1n[t])/(Jiwee + Wee1n[t])
R42 = (kawee*nuclei*Wee1Pn[t])/(Jawee + Wee1Pn[t])
R43 = (nuclei*(kastgp + kastg*MPFn[t])*Stgn[t])/(Jastg + Stgn[t])
R44 = (kistg*nuclei*StgPn[t])/(Jistg + StgPn[t])
R45 = kdstg*nuclei*Stgn[t]
R46 = kdstg*nuclei*StgPn[t]
R6 = cytoplasm*kdc*preMPFc[t]
R7 = cytoplasm*kdc*MPFc[t]
R8 = (cytoplasm*(kiweep + kiwee*MPFc[t])*Wee1c[t])/(Jiwee + Wee1c[t])
R9 = (cytoplasm*kawee*Wee1Pc)/(Jawee + Wee1Pc)
16 = cytoplasm*kt*MPFc[t]
18 = cytoplasm*kt*preMPFc[t]
1821 = (E1*koutw1*nuclei*N[t]*Wee1Pn[t])/(1 - E1*N[t])
20 = cytoplasm*kinw1*Wee1Pc
22 = (E1*koutw1*nuclei*N[t]*Wee1n[t])/(1 - E1*N[t])
24 = cytoplasm*kinw1*Wee1c[t]
26 = (E1*kouts1*nuclei*N[t]*StgPn[t])/(1 - E1*N[t])
28 = cytoplasm*kins1*StgPc[t]
30 = (E1*kouts1*nuclei*N[t]*Stgn[t])/(1 - E1*N[t])
32 = cytoplasm*kins1*Stgc[t]
50 = ksxm1*nuclei*N[t]
51 = cytoplasm*ksxp1*Xm[t]
exportofMPFfromnucleus1 = kout1*nuclei*MPFn[t]
exportofpreMPFnfromnucleus1 = kout1*nuclei*preMPFn[t]
importofMPFintocytoplasm1 = (E1*kout1*nuclei*MPFn[t]*N[t])/(1 - E1*N[t])
importofpreMPFintocytoplaslm1 = (E1*kout1*nuclei*N[t]*preMPFn[t])/(1 - E1*N[t])
end rate equations

begin parameters
E1 = "7e-05"
Jafzy = 0.01
Jaie = 0.01
Jastg = 0.05
Jawee = 0.05
Jifzy = 0.01
Jiie = 0.01
Jistg = 0.05
Jiwee = 0.05
Jm = 0.05
StringT = 0.8
Wee1T = 0.8
factor1 = 1.95
kafzy = 1.0
kaie = 1.0
kastg = 1.0
kastgp = 0.0
kawee = 0.3
kdc = 0.01
kdm = 0.2
kdmp = 0.002
kdn = 1.5
kdnp = 0.01
kdstg = 0.0
kez1 = 0.5
kifzy = 0.2
kiie = 0.4
kins1 = 0.08
kinw1 = 0.04
kistg = 0.3
kiwee = 1.0
kiweep = 0.01
kout1 = 0.0
kouts1 = 0.02
koutw1 = 0.01
ksc = 0.01
ksstg = 0.0
kstg = 2.0
kstgp = 0.2
ksxm1 = 0.0005
ksxp1 = 0.001
kt = 0.15
kwee = 1.0
kweep = 0.005
cytoplasm = 1.0
nuclei = 1.0
end parameters

begin initial conditions
FZYa[0] = FZYai
IEa1[0] = IEa1i
MPFc[0] = MPFci
MPFn[0] = MPFni
N[0] = Ni
StgPc[0] = StgPci
StgPn[0] = StgPni
Stgc[0] = Stgci
Stgm[0] = Stgmi
Stgn[0] = Stgni
Wee1Pn[0] = Wee1Pni
Wee1c[0] = Wee1ci
Wee1n[0] = Wee1ni
Xm[0] = Xmi
Xp[0] = Xpi
preMPFc[0] = preMPFci
preMPFn[0] = preMPFni
end initial conditions

begin initial values
FZYai = 0.0
IEa1i = 0.0
MPFci = 1.0
MPFni = 0.0
Ni = 1.0
StgPci = 0.8
StgPni = 0.0
Stgci = 0.0
Stgmi = 1.0
Stgni = 0.0
Wee1Pni = 0.0
Wee1ci = 0.0
Wee1ni = 0.0
Xmi = 0.0
Xpi = 0.0
preMPFci = 0.0
preMPFni = 0.0
end initial values

begin assignment rules
Wee1Pc := -Wee1c[t] + (Wee1T - E1*N[t]*(Wee1n[t] + Wee1Pn[t]))/(1 - E1*N[t])
StgPT := (1 - E1*N[t])*StgPc[t] + E1*N[t]*StgPn[t]
CycBT := (1 - E1*N[t])*(MPFc[t] + preMPFc[t]) + E1*N[t]*(MPFn[t] + preMPFn[t])
end assignment rules

begin function definitions
end function definitions

begin events
unnamed = (FZYa[t] >= kez1, {MPFc[t] -> (MPFc[t]*(1 - E1*N[t]))/(1 - 1.95*E1*N[t]), StgPn[t] -> 0.5128205128205129*StgPn[t], Stgc[t] -> ((1 - E1*N[t])*Stgc[t])/(1 - 1.95*E1*N[t]), StgPc[t] -> ((1 - E1*N[t])*StgPc[t])/(1 - 1.95*E1*N[t]), Stgn[t] -> 0.5128205128205129*Stgn[t], Wee1c[t] -> ((1 - E1*N[t])*Wee1c[t])/(1 - 1.95*E1*N[t]), Wee1Pn[t] -> 0.5128205128205129*Wee1Pn[t], Wee1n[t] -> 0.5128205128205129*Wee1n[t], preMPFn[t] -> 0.5128205128205129*preMPFn[t], MPFn[t] -> 0.5128205128205129*MPFn[t], N[t] -> factor1*N[t]}, 0)
end events

begin process annotations
end process annotations

begin species annotations
FZYa http://identifiers.org/uniprot/Q24044
FZYa http://identifiers.org/interpro/IPR000002
StgPc http://identifiers.org/uniprot/P20483
StgPn http://identifiers.org/uniprot/P20483
StgPn http://identifiers.org/interpro/IPR000751
Stgc http://identifiers.org/uniprot/P20483
Stgn http://identifiers.org/uniprot/P20483
Stgn http://identifiers.org/interpro/IPR000751
Wee1Pc http://identifiers.org/uniprot/P54350
Wee1Pc http://identifiers.org/uniprot/P30291
Wee1Pn http://identifiers.org/uniprot/P54350
Wee1Pn http://identifiers.org/uniprot/P30291
Wee1c http://identifiers.org/uniprot/P54350
Wee1c http://identifiers.org/uniprot/P30291
Wee1n http://identifiers.org/uniprot/P54350
Wee1n http://identifiers.org/uniprot/P30291
end species annotations

begin units
end units