Initial concentration set to 60 pM (in line with insulin secretion).

 1000 cell 0.95 cell 0.05 cell atp adp atp adp 1 -8 nadh nad nadh nad 1 -8 fbp grap fbp dhap grap fbp dhap lac pyr 10.1746465657385 glc_ext 2.4 10.1746465657385 0.184822580052604 glc_ext 2.4 glc_ext 2.4 glc_ext 4 9.87893043765431 0.184822580052604 glc_ext 4 glc_ext 4 glc_ext 8.6 9.02874656941233 0.184822580052604 glc_ext 8.6 glc_ext 8.6 glc_ext 14.5 7.93829334710197 0.184822580052604 glc_ext 14.5 glc_ext 14.5 glc_ext 16 7.66105947702306 glc_ext 16 0 0.3 glc_ext 1.39 glc_ext 1.39 5.32 1.39 0.59 0.23 glc_ext 1.53 glc_ext 1.53 9.13 1.53 0.13

rateOf(ATP) rate of change

 ATPformation ATPconsumption PGK PYK PFK26 HEX1 PFK ATP_ADP_CHANGE IRS cell 1.87 glc_ext 2.03 glc_ext 2.03 4.01 2.03 4.66

Steady state insulin secretion (IRS) depending on ATP/ADP ratio. Uses a Hill function.

 IRSss_Vm atp adp IRSss_hillatp_ratio atp adp IRSss_hillatp_ratio IRSss_Katp_ratio IRSss_hillatp_ratio

First phase insulin secretion (IRS) depending on the change in ATP: dATP/dt. The rise in ATP concentration triggers closure of APT-sensitive potassium (KATP) channels, which ultimately result in the release of insulin in the bloodstream.

 IRSfp_Vm datp_dt datp_dt IRSfp_Kdatp_dt datp_dt 0 0 cyto NUCLEOTIDE_CHANGE_Vm atp_adp_ss atp_adp cyto ATPconsumption_Vm atp ATPconsumption_KMatp 1 atp ATPconsumption_KMatp cyto ATPformation_Vm adp phos ATPformation_KMadp ATPformation_KMphos 1 adp ATPformation_KMadp 1 phos ATPformation_KMphos cyto ENO_Vm 1 ENO_KMpg2 pg2 pep ENO_Keq 1 pg2 ENO_KMpg2 1 pep ENO_KMpep 1 cyto FBA_Vm 1 FBA_KMfbp fbp dhap grap FBA_Keq 1 fbp FBA_KMfbp 1 dhap FBA_KMdhap 1 grap FBA_KMgrap 1 cyto FBP_Vm fbp FBP_KMfbp 1 fbp FBP_KMfbp cyto FBP26_Vm f26bp FBP26_KMf26bp 1 f26bp FBP26_KMf26bp cyto GAPD_Vm 1 GAPD_KMgrap GAPD_KMphos GAPD_KMnad grap phos nad bpg13 nadh GAPD_Keq 1 grap GAPD_KMgrap 1 phos GAPD_KMphos 1 nad GAPD_KMnad 1 bpg13 GAPD_KMbpg13 1 nadh GAPD_KMnadh 1 cyto GLCt1r_Vm 1 GLCt1r_KMglc_ext glc_ext glc GLCt1r_Keq 1 glc_ext GLCt1r_KMglc_ext 1 glc GLCt1r_KMglc 1 cyto HEX1_Vm 1 HEX1_KMglc HEX1_KMatp glc atp g6p adp HEX1_Keq 1 glc HEX1_KMglc 1 atp HEX1_KMatp 1 g6p HEX1_KMg6p 1 adp HEX1_KMadp 1 cyto IRS_ss IRS_fp cyto LDH_L_Vm 1 LDH_L_KMpyr LDH_L_KMnadh pyr nadh lac nad LDH_L_Keq 1 pyr LDH_L_KMpyr 1 nadh LDH_L_KMnadh 1 lac LDH_L_KMlac 1 nad LDH_L_KMnad 1 cyto L_LACt2r_Vm lac L_LACt2r_KMlac 1 lac L_LACt2r_KMlac cyto PFK_Vm f6p atp PFK_KMf6p PFK_KMatp 1 f6p PFK_KMf6p 1 atp PFK_KMatp cyto PFK26_Vm atp f6p PFK26_KMatp PFK26_KMf6p 1 atp PFK26_KMatp 1 f6p PFK26_KMf6p cyto PGI_Vm 1 PGI_KMg6p g6p f6p PGI_Keq 1 g6p PGI_KMg6p 1 f6p PGI_KMf6p 1 cyto PGK_Vm 1 PGK_KMadp PGK_KMbpg13 adp bpg13 atp pg3 PGK_Keq 1 adp PGK_KMadp 1 bpg13 PGK_KMbpg13 1 atp PGK_KMatp 1 pg3 PGK_KMpg3 1 cyto PGM_Vm 1 PGM_KMpg3 pg3 pg2 PGM_Keq 1 pg3 PGM_KMpg3 1 pg2 PGM_KMpg2 1 cyto PYK_Vm 1 PYK_KMpep PYK_KMadp pep adp pyr atp PYK_Keq 1 pep PYK_KMpep 1 adp PYK_KMadp 1 pyr PYK_KMpyr 1 atp PYK_KMatp 1 cyto PYRt2r_Vm pyr PYRt2r_KMpyr 1 pyr PYRt2r_KMpyr cyto TPI_Vm 1 TPI_KMdhap dhap grap TPI_Keq 1 dhap TPI_KMdhap 1 grap TPI_KMgrap 1

Event added to model to model simulation under given glucose concentration for parameter fitting.

 time presim_duration sim_glc_ext

Event for presimulation under given glucose concentration for parameter fitting.

 presim_duration 0 time presim_duration presim_glc_ext