JWS Online

Model

Name

machado1

Notes

The SBML for this model was obtained from the BioModels database (BioModels ID: BIOMD0000000565) Biomodels notes: Figure 4 of the reference paper has been reproduced here. Note that the difference in the y-axis measure is due to the difference in units. The y-axis is in mmol/L in the model, whereas it is in g/L in the paper. The model as such reproduces the Mutant_0 condition. To simulate the Mutant_1 and Mutant_2 condition, do the following. Mutant_1 - set rmaxACCOAC = 0.4634 Mutant_2 - set rmaxACCOAC = 0.4634; rmaxPK = 0.6113150258 JWS Online curation: This model was curated by reproducing the figures as described in the BioModels Notes. No additional changes were made.

Substance units

None

Time units

None

Volume units

None

Area units

None

Length units

None

Reaction extent units

None

Manuscript information

A kinetic model for curcumin production in Escherichia coli.

Daniel Machado
Lígia R Rodrigues
Isabel Rocha

BioSystems 2014; 125 : 16

PubMed: 25218090
DOI: 10.1016/j.biosystems.2014.09.001
ISSN: 1872-8324
URL: https://ncbi.nlm.nih.gov/pubmed/25218090

Abstract

Curcumin is a natural compound obtained from turmeric, and is well known for its pharmacological effects. In this work, we design a heterologous pathway for industrial production of curcumin in Escherichia coli. A kinetic model of the pathway is then developed and connected to a kinetic model of the central carbon metabolism of E. coli. This model is used for optimization of the mutant strain through a rational design approach, and two manipulation targets are identified for overexpression. Dynamic simulations are then performed to compare the curcumin production profiles of the different mutant strains. Our results show that it is possible to obtain a significant improvement in the curcumin production rates with the proposed mutants. The kinetic model here developed can be an important framework to optimize curcumin production at an industrial scale and add value to its biomedical potential.

Simulations using this model 3

Simulation
machado2014_Fig4	SED-ML COMBINE Archive
machado2014_Fig4	SED-ML COMBINE Archive
machado2014_Fig4	SED-ML COMBINE Archive

Unit definitions 6

Unit definitions have no effect on the numerical analysis of the model. It remains the responsibility of the modeler to ensure the internal numerical consistency of the model. If units are provided, however, the consistency of the model units will be checked.

Name	Definition
—	0.001 mole
—	0.001 mole litre^(-1.0)
—	1.0 second^(-1.0)
—	0.001 mole litre^(-1.0) second^(-1.0)
—	1000.0 mole^(-1.0) litre second^(-1.0)
—	1.0 dimensionless

Compartments 2

Id	Name	Spatial dimensions	Size
cytosol	—	3.0	1.0
extracellular	—	3.0	65.0

Species 26

Id	Name	Initial quantity	Compartment	Fixed
accoa	—	1.0	cytosol	✘
cdhap	Dihydroxyacetonephosphate	0.167	cytosol	✘
ce4p	Erythrose-4-phosphate	0.098	cytosol	✘
cf6p	Fructose-6-Phosphate	0.6	cytosol	✘
cfdp	Fructose-1,6-bisphosphate	0.272	cytosol	✘
cg1p	Glucose-1-Phosphate	0.653	cytosol	✘
cg6p	Glucose-6-Phosphate	3.48	cytosol	✘
cgap	Glyceraldehyde-3-Phosphate	0.218	cytosol	✘
cglcex	Extracellular Glucose	55.5	extracellular	✘
cpep	Phosphoenol pyruvate	2.67	cytosol	✘
cpg	6-Phosphogluconate	0.808	cytosol	✘
cpg2	2-Phosphoglycerate	0.399	cytosol	✘
cpg3	3-Phosphoglycerate	2.13	cytosol	✘
cpgp	1,3-diphosphosphoglycerate	0.008	cytosol	✘
cpyr	Pyruvate	2.67	cytosol	✘
crib5p	Ribose-5-phosphate	0.398	cytosol	✘
cribu5p	Ribulose-5-phosphate	0.111	cytosol	✘
csed7p	sedoheptulose-7-phosphate	0.276	cytosol	✘
cur	—	0.0	cytosol	✘
cur_ext	—	0.0	extracellular	✘
cxyl5p	Xylulose-5-phosphate	0.138	cytosol	✘
fer	—	0.0	cytosol	✘
fer_ext	—	271.5	extracellular	✘
fercoa	—	0.0	cytosol	✘
ferdicoa	—	0.0	cytosol	✘
malcoa	—	1.0	cytosol	✘

Initial assignments 0

Initial assignments are expressions that are evaluated at time=0. It is not recommended to create initial assignments for all model entities. Restrict the use of initial assignments to cases where a value is expressed in terms of values or sizes of other model entities. Note that it is not permitted to have both an initial assignment and an assignment rule for a single model entity.

Definition

Reactions 64

Id	Name	Reaction Equation and Kinetic Law
ACCOAC	—	accoa > malcoa cytosol * rmaxACCOAC * accoa / (K_ACCOAC_accoa * (1 + malcoa / Ki_ACCOAC_malcoa) + accoa)
CURS	—	fercoa + ferdicoa > cur E_CURS * kcat_CURS * (fercoa / (Km_CURS_fercoa + fercoa)) * (ferdicoa / (Km_CURS_ferdicoa + ferdicoa))
CUR_t	—	cur = cur_ext cytosol * k_CUR_t * (cur - cur_ext / Keq_CUR_t)
DCS	—	fercoa + malcoa > ferdicoa E_DCS * kcat_DCS * (pow(fercoa, n_DCS_fercoa) / (pow(Km_DCS_fercoa, n_DCS_fercoa) + pow(fercoa, n_DCS_fercoa))) * (malcoa / (Km_DCS_malcoa + malcoa))
EX_CUR	—	cur_ext > ∅ extracellular * Dil * cur_ext
EX_FER	—	∅ > fer_ext extracellular * Dil * (cfeed_fer - fer_ext)
FER_t	—	fer_ext = fer extracellular * k_FER_t * (fer_ext - fer / Keq_FER_t)
R4CL	—	fer > fercoa E_4CL * kcat_4CL * fer / (Km_4CL + fer)
Synth3	Synthesis 3	accoa > ∅ cytosol * rmaxSynth3 * accoa / (KSynth3accoa + accoa)
Synth4	Synthesis 4	malcoa > ∅ cytosol * rmaxSynth4 * malcoa / (KSynth4malcoa + malcoa)
vACCOA	ACCOA dilution	accoa = ∅ cytosol * Dil * accoa
vALDO	Aldolase	cfdp = cdhap + cgap cytosol * rmaxALDO * (cfdp - cgap * cdhap / kALDOeq) / (kALDOfdp + cfdp + kALDOgap * cdhap / (kALDOeq * VALDOblf) + kALDOdhap * cgap / (kALDOeq * VALDOblf) + cfdp * cgap / kALDOgapinh + cgap * cdhap / (VALDOblf * kALDOeq))
vCUR	CUR dilution	cur = ∅ cytosol * Dil * cur
vDAHPS	DAHP synthesis	ce4p + cpep > ∅ cytosol * rmaxDAHPS * pow(ce4p, nDAHPSe4p) * pow(cpep, nDAHPSpep) / ((KDAHPSe4p + pow(ce4p, nDAHPSe4p)) * (KDAHPSpep + pow(cpep, nDAHPSpep)))
vDHAP	DHAP degradation	cdhap = ∅ cytosol * Dil * cdhap
vE4P	E4P dilution	ce4p = ∅ cytosol * Dil * ce4p
vENO	Enolase	cpg2 = cpep cytosol * rmaxENO * (cpg2 - cpep / KENOeq) / (KENOpg2 * (1 + cpep / KENOpep) + cpg2)
vEXTER	Extracellular glucose kinetics	∅ > cglcex extracellular * Dil * (cfeed_glc - cglcex)
vFER	FER dilution	fer = ∅ cytosol * Dil * fer
vFERCOA	FERCOA dilution	fercoa = ∅ cytosol * Dil * fercoa
vFERDICOA	FERDICOA dilution	ferdicoa = ∅ cytosol * Dil * ferdicoa
vG1PAT	Glucose-1-phosphate adenyltransferase	cg1p > ∅ cytosol * rmaxG1PAT * cg1p * catp * (1 + pow(cfdp / KG1PATfdp, nG1PATfdp)) / ((KG1PATatp + catp) * (KG1PATg1p + cg1p))
vG3PDH	Glycerol-3-phosphate dehydrogenase	cdhap > ∅ cytosol * rmaxG3PDH * cdhap / (KG3PDHdhap + cdhap)
vG6P	G6P degradation	cg6p = ∅ cytosol * Dil * cg6p
vG6PDH	Glucose-6-phosphate dehydrogenase	cg6p > cpg cytosol * rmaxG6PDH * cg6p * cnadp / ((cg6p + KG6PDHg6p) * (1 + cnadph / KG6PDHnadphg6pinh) * (KG6PDHnadp * (1 + cnadph / KG6PDHnadphnadpinh) + cnadp))
vGAP	GAP degradation	cgap = ∅ cytosol * Dil * cgap
vGAPDH	Glyceraldehyde-3-phosphate dehydrogenase	cgap = cpgp cytosol * rmaxGAPDH * (cgap * cnad - cpgp * cnadh / KGAPDHeq) / ((KGAPDHgap * (1 + cpgp / KGAPDHpgp) + cgap) * (KGAPDHnad * (1 + cnadh / KGAPDHnadh) + cnad))
vGLP	GLP dilution	cg1p = ∅ cytosol * Dil * cg1p
vMALCOA	MALCOA dilution	malcoa = ∅ cytosol * Dil * malcoa
vMURSyNTH	Mureine synthesis	{2.0}cf6p > ∅ cytosol * rmaxMurSynth
vMethSynth	Methionine synthesis	∅ > cpyr cytosol * rmaxMetSynth
vPDH	Pyruvate dehydrogenase	cpyr > accoa cytosol * rmaxPDH * pow(cpyr, nPDH) / (KPDHpyr * (1 + accoa / Ki_PDH_accoa) + pow(cpyr, nPDH))
vPEP	PEP degradation	cpep = ∅ cytosol * Dil * cpep
vPFK	Phosphofructokinase	cf6p > cfdp cytosol * rmaxPFK * catp * cf6p / ((catp + KPFKatps * (1 + cadp / KPFKadpc)) * (cf6p + KPFKf6ps * (1 + cpep / KPFKpep + cadp / KPFKadpb + camp / KPFKampb) / (1 + cadp / KPFKadpa + camp / KPFKampa)) * (1 + LPFK / pow(1 + cf6p * (1 + cadp / KPFKadpa + camp / KPFKampa) / (KPFKf6ps * (1 + cpep / KPFKpep + cadp / KPFKadpb + camp / KPFKampb)), nPFK)))
vPG	PG dilution	cpg = ∅ cytosol * Dil * cpg
vPG3	PG3 degradation	cpg3 = ∅ cytosol * Dil * cpg3
vPGDH	6-Phosphogluconate dehydrogenase	cpg > cribu5p cytosol * rmaxPGDH * cpg * cnadp / ((cpg + KPGDHpg) * (cnadp + KPGDHnadp * (1 + cnadph / KPGDHnadphinh) * (1 + catp / KPGDHatpinh)))
vPGI	Glucose-6-phosphate isomerase	cg6p = cf6p cytosol * rmaxPGI * (cg6p - cf6p / KPGIeq) / (KPGIg6p * (1 + cf6p / (KPGIf6p * (1 + cpg / KPGIf6ppginh)) + cpg / KPGIg6ppginh) + cg6p)
vPGK	Phosphoglycerate kinase	cpgp = cpg3 cytosol * rmaxPGK * (cadp * cpgp - catp * cpg3 / KPGKeq) / ((KPGKadp * (1 + catp / KPGKatp) + cadp) * (KPGKpgp * (1 + cpg3 / KPGKpg3) + cpgp))
vPGM	Phosphoglucomutase	cg6p = cg1p cytosol * rmaxPGM * (cg6p - cg1p / KPGMeq) / (KPGMg6p * (1 + cg1p / KPGMg1p) + cg6p)
vPGP	PGP degradation	cpgp = ∅ cytosol * Dil * cpgp
vPK	Pyruvate kinase	cpep > cpyr cytosol * rmaxPK * cpep * pow(cpep / KPKpep + 1, nPK - 1) * cadp / (KPKpep * (LPK * pow((1 + catp / KPKatp) / (cfdp / KPKfdp + camp / KPKamp + 1), nPK) + pow(cpep / KPKpep + 1, nPK)) * (cadp + KPKadp))
vPPK	Ribose phosphate pyrophosphokinase	crib5p > ∅ cytosol * rmaxRPPK * crib5p / (KRPPKrib5p + crib5p)
vPTS	Phosphotransferase system	cglcex + cpep > cg6p + cpyr extracellular * rmaxPTS * cglcex * (cpep / cpyr) / ((KPTSa1 + KPTSa2 * (cpep / cpyr) + KPTSa3 * cglcex + cglcex * (cpep / cpyr)) * (1 + pow(cg6p, nPTSg6p) / KPTSg6p))
vR5PI	Ribose-phosphate isomerase	cribu5p = crib5p cytosol * rmaxR5PI * (cribu5p - crib5p / KR5PIeq)
vRIB5P	Rib5P dilution	crib5p = ∅ cytosol * Dil * crib5p
vRibu5p	Ribu5P dilution	cribu5p = ∅ cytosol * Dil * cribu5p
vRu5P	Ribulose-phosphate epimerase	cribu5p = cxyl5p cytosol * rmaxRu5P * (cribu5p - cxyl5p / KRu5Peq)
vSED7P	SED7P dilution	csed7p = ∅ cytosol * Dil * csed7p
vSynth1	Synthesis 1	cpep > ∅ cytosol * rmaxSynth1 * cpep / (KSynth1pep + cpep)
vSynth2	Synthesis 2	cpyr > ∅ cytosol * rmaxSynth2 * cpyr / (KSynth2pyr + cpyr)
vTA	Transaldolase	cgap + csed7p = cf6p + ce4p cytosol * rmaxTA * (cgap * csed7p - ce4p * cf6p / KTAeq)
vTIS	Triosephosphate isomerase	cdhap = cgap cytosol * rmaxTIS * (cdhap - cgap / kTISeq) / (kTISdhap * (1 + cgap / kTISgap) + cdhap)
vTKA	Transketolase a	crib5p + cxyl5p = cgap + csed7p cytosol * rmaxTKa * (crib5p * cxyl5p - csed7p * cgap / KTKaeq)
vTKB	Transketolase b	ce4p + cxyl5p = cgap + cf6p cytosol * rmaxTKb * (cxyl5p * ce4p - cf6p * cgap / KTKbeq)
vTRPSYNTH	Tryptophan synthesis	∅ > cpyr + cgap cytosol * rmaxTrpSynth
vXYL5P	XYL5P dilution	cxyl5p = ∅ cytosol * Dil * cxyl5p
vf6P	F6P degradation	cf6p = ∅ cytosol * Dil * cf6p
vfdP	FDP degradation	cfdp = ∅ cytosol * Dil * cfdp
vpepCxylase	PEP carboxylase	cpep > ∅ cytosol * rmaxpepCxylase * cpep * (1 + pow(cfdp / KpepCxylasefdp, npepCxylasefdp)) / (KpepCxylasepep + cpep)
vpg2	PG2 degradation	cpg2 = ∅ cytosol * Dil * cpg2
vpyr	Pyruvate dilution	cpyr = ∅ cytosol * Dil * cpyr
vrpGluMu	Phosphoglycerate mutase	cpg3 = cpg2 cytosol * rmaxPGluMu * (cpg3 - cpg2 / KPGluMueq) / (KPGluMupg3 * (1 + cpg2 / KPGluMupg2) + cpg3)
vsersynth	Serine synthesis	cpg3 > ∅ cytosol * rmaxSerSynth * cpg3 / (KSerSynthpg3 + cpg3)

Parameters 142 8

Global parameters

Id	Value
Dil	0.0 second inverse
cadp	0.595
camp	0.955
catp	4.27
cnad	1.47
cnadh	0.1
cnadp	0.195
cnadph	0.062

Local parameters

Id	Value	Reaction
rmaxPTS	7829.78 mM per second	vPTS (Phosphotransferase system)
KPTSa1	3082.3	vPTS (Phosphotransferase system)
KPTSa2	0.01	vPTS (Phosphotransferase system)
KPTSa3	245.3 dimensionless	vPTS (Phosphotransferase system)
nPTSg6p	3.66 dimensionless	vPTS (Phosphotransferase system)
KPTSg6p	2.15	vPTS (Phosphotransferase system)
rmaxPGI	650.9878687 mM per second	vPGI (Glucose-6-phosphate isomerase)
KPGIeq	0.1725 dimensionless	vPGI (Glucose-6-phosphate isomerase)
KPGIg6p	2.9	vPGI (Glucose-6-phosphate isomerase)
KPGIf6p	0.266	vPGI (Glucose-6-phosphate isomerase)
KPGIf6ppginh	0.2	vPGI (Glucose-6-phosphate isomerase)
KPGIg6ppginh	0.2	vPGI (Glucose-6-phosphate isomerase)
rmaxPGM	0.8398242773 mM per second	vPGM (Phosphoglucomutase)
KPGMeq	0.196 dimensionless	vPGM (Phosphoglucomutase)
KPGMg6p	1.038	vPGM (Phosphoglucomutase)
KPGMg1p	0.0136	vPGM (Phosphoglucomutase)
rmaxG6PDH	1.380196955 mM per second	vG6PDH (Glucose-6-phosphate dehydrogenase)
KG6PDHg6p	14.4	vG6PDH (Glucose-6-phosphate dehydrogenase)
KG6PDHnadphg6pinh	6.43	vG6PDH (Glucose-6-phosphate dehydrogenase)
KG6PDHnadp	0.0246	vG6PDH (Glucose-6-phosphate dehydrogenase)
KG6PDHnadphnadpinh	0.01	vG6PDH (Glucose-6-phosphate dehydrogenase)
rmaxPFK	1840.584747 mM per second	vPFK (Phosphofructokinase)
KPFKatps	0.123	vPFK (Phosphofructokinase)
KPFKadpc	4.14	vPFK (Phosphofructokinase)
KPFKf6ps	0.325	vPFK (Phosphofructokinase)
KPFKpep	3.26	vPFK (Phosphofructokinase)
KPFKadpb	3.89	vPFK (Phosphofructokinase)
KPFKampb	3.2	vPFK (Phosphofructokinase)
KPFKadpa	128.0	vPFK (Phosphofructokinase)
KPFKampa	19.1	vPFK (Phosphofructokinase)
LPFK	5629067.0 dimensionless	vPFK (Phosphofructokinase)
nPFK	11.1 dimensionless	vPFK (Phosphofructokinase)
rmaxTA	10.87164108 per mM per second	vTA (Transaldolase)
KTAeq	1.05 dimensionless	vTA (Transaldolase)
rmaxTKa	9.473384783 per mM per second	vTKA (Transketolase a)
KTKaeq	1.2 dimensionless	vTKA (Transketolase a)
rmaxTKb	86.55855855 per mM per second	vTKB (Transketolase b)
KTKbeq	10.0 dimensionless	vTKB (Transketolase b)
rmaxMurSynth	0.0 mM per second	vMURSyNTH (Mureine synthesis)
rmaxALDO	17.41464425 mM per second	vALDO (Aldolase)
kALDOeq	0.144	vALDO (Aldolase)
kALDOfdp	1.75	vALDO (Aldolase)
kALDOgap	0.088	vALDO (Aldolase)
VALDOblf	2.0 dimensionless	vALDO (Aldolase)
kALDOdhap	0.088	vALDO (Aldolase)
kALDOgapinh	0.6	vALDO (Aldolase)
rmaxGAPDH	921.5942861 mM per second	vGAPDH (Glyceraldehyde-3-phosphate dehydrogenase)
KGAPDHeq	0.63 dimensionless	vGAPDH (Glyceraldehyde-3-phosphate dehydrogenase)
KGAPDHgap	0.683	vGAPDH (Glyceraldehyde-3-phosphate dehydrogenase)
KGAPDHpgp	0.0000104	vGAPDH (Glyceraldehyde-3-phosphate dehydrogenase)
KGAPDHnad	0.252	vGAPDH (Glyceraldehyde-3-phosphate dehydrogenase)
KGAPDHnadh	1.09	vGAPDH (Glyceraldehyde-3-phosphate dehydrogenase)
rmaxTIS	68.67474392 mM per second	vTIS (Triosephosphate isomerase)
kTISeq	1.39 dimensionless	vTIS (Triosephosphate isomerase)
kTISdhap	2.8	vTIS (Triosephosphate isomerase)
kTISgap	0.3	vTIS (Triosephosphate isomerase)
rmaxTrpSynth	0.001037 mM per second	vTRPSYNTH (Tryptophan synthesis)
rmaxG3PDH	0.01162042696 mM per second	vG3PDH (Glycerol-3-phosphate dehydrogenase)
KG3PDHdhap	1.0	vG3PDH (Glycerol-3-phosphate dehydrogenase)
rmaxPGK	3021.773771 mM per second	vPGK (Phosphoglycerate kinase)
KPGKeq	1934.4 dimensionless	vPGK (Phosphoglycerate kinase)
KPGKadp	0.185	vPGK (Phosphoglycerate kinase)
KPGKatp	0.653	vPGK (Phosphoglycerate kinase)
KPGKpgp	0.0468	vPGK (Phosphoglycerate kinase)
KPGKpg3	0.473	vPGK (Phosphoglycerate kinase)
rmaxSerSynth	0.025712107 mM per second	vsersynth (Serine synthesis)
KSerSynthpg3	1.0	vsersynth (Serine synthesis)
rmaxPGluMu	89.04965407 mM per second	vrpGluMu (Phosphoglycerate mutase)
KPGluMueq	0.188 dimensionless	vrpGluMu (Phosphoglycerate mutase)
KPGluMupg3	0.2	vrpGluMu (Phosphoglycerate mutase)
KPGluMupg2	0.369	vrpGluMu (Phosphoglycerate mutase)
rmaxENO	330.4476151 mM per second	vENO (Enolase)
KENOeq	6.73	vENO (Enolase)
KENOpg2	0.1	vENO (Enolase)
KENOpep	0.135	vENO (Enolase)
rmaxPK	0.06113150238 mM per second	vPK (Pyruvate kinase)
KPKpep	0.31	vPK (Pyruvate kinase)
nPK	4.0 dimensionless	vPK (Pyruvate kinase)
LPK	1000.0 dimensionless	vPK (Pyruvate kinase)
KPKatp	22.5	vPK (Pyruvate kinase)
KPKfdp	0.19	vPK (Pyruvate kinase)
KPKamp	0.2	vPK (Pyruvate kinase)
KPKadp	0.26	vPK (Pyruvate kinase)
rmaxpepCxylase	0.1070205858 mM per second	vpepCxylase (PEP carboxylase)
KpepCxylasefdp	0.7	vpepCxylase (PEP carboxylase)
npepCxylasefdp	4.21 dimensionless	vpepCxylase (PEP carboxylase)
KpepCxylasepep	4.07	vpepCxylase (PEP carboxylase)
rmaxSynth1	0.01953897003 mM per second	vSynth1 (Synthesis 1)
KSynth1pep	1.0	vSynth1 (Synthesis 1)
rmaxSynth2	0.07361855055 mM per second	vSynth2 (Synthesis 2)
KSynth2pyr	1.0	vSynth2 (Synthesis 2)
rmaxDAHPS	0.1079531227 mM per second	vDAHPS (DAHP synthesis)
nDAHPSe4p	2.6 dimensionless	vDAHPS (DAHP synthesis)
nDAHPSpep	2.2 dimensionless	vDAHPS (DAHP synthesis)
KDAHPSe4p	0.035	vDAHPS (DAHP synthesis)
KDAHPSpep	0.0053	vDAHPS (DAHP synthesis)
rmaxPDH	270.27734 mM per second	vPDH (Pyruvate dehydrogenase)
nPDH	3.68 dimensionless	vPDH (Pyruvate dehydrogenase)
KPDHpyr	1159.0	vPDH (Pyruvate dehydrogenase)
Ki_PDH_accoa	0.0222222	vPDH (Pyruvate dehydrogenase)
rmaxMetSynth	0.0022627 mM per second	vMethSynth (Methionine synthesis)
rmaxPGDH	16.23235977 mM per second	vPGDH (6-Phosphogluconate dehydrogenase)
KPGDHpg	37.5	vPGDH (6-Phosphogluconate dehydrogenase)
KPGDHnadp	0.0506	vPGDH (6-Phosphogluconate dehydrogenase)
KPGDHnadphinh	0.0138	vPGDH (6-Phosphogluconate dehydrogenase)
KPGDHatpinh	208.0	vPGDH (6-Phosphogluconate dehydrogenase)
rmaxR5PI	4.83841193 second inverse	vR5PI (Ribose-phosphate isomerase)
KR5PIeq	4.0 dimensionless	vR5PI (Ribose-phosphate isomerase)
rmaxRu5P	6.739029475 second inverse	vRu5P (Ribulose-phosphate epimerase)
KRu5Peq	1.4 dimensionless	vRu5P (Ribulose-phosphate epimerase)
rmaxRPPK	0.01290045226 mM per second	vPPK (Ribose phosphate pyrophosphokinase)
KRPPKrib5p	0.1	vPPK (Ribose phosphate pyrophosphokinase)
rmaxG1PAT	0.007525458026 mM per second	vG1PAT (Glucose-1-phosphate adenyltransferase)
KG1PATfdp	0.119	vG1PAT (Glucose-1-phosphate adenyltransferase)
nG1PATfdp	1.2	vG1PAT (Glucose-1-phosphate adenyltransferase)
KG1PATatp	4.42	vG1PAT (Glucose-1-phosphate adenyltransferase)
KG1PATg1p	3.2	vG1PAT (Glucose-1-phosphate adenyltransferase)
cfeed_glc	110.96	vEXTER (Extracellular glucose kinetics)
rmaxACCOAC	0.04634 mM per second	ACCOAC
K_ACCOAC_accoa	0.0003	ACCOAC
Ki_ACCOAC_malcoa	0.1	ACCOAC
rmaxSynth3	0.284 mM per second	Synth3 (Synthesis 3)
KSynth3accoa	1.0	Synth3 (Synthesis 3)
rmaxSynth4	0.092372 mM per second	Synth4 (Synthesis 4)
KSynth4malcoa	1.0	Synth4 (Synthesis 4)
E_4CL	100.0	R4CL
kcat_4CL	9.572	R4CL
Km_4CL	0.026	R4CL
E_DCS	100.0	DCS
kcat_DCS	0.01343	DCS
Km_DCS_fercoa	0.046	DCS
Km_DCS_malcoa	0.0084	DCS
n_DCS_fercoa	1.8	DCS
E_CURS	100.0	CURS
kcat_CURS	0.02163	CURS
Km_CURS_fercoa	0.018	CURS
Km_CURS_ferdicoa	0.018	CURS
k_FER_t	1000.0 second inverse	FER_t
Keq_FER_t	1.0 dimensionless	FER_t
k_CUR_t	1000.0 second inverse	CUR_t
Keq_CUR_t	1.0 dimensionless	CUR_t
cfeed_fer	500.0	EX_FER

Rules 0 0 0

Assignment rules

Definition

Rate rules

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Algebraic rules

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Function definitions 0

Definition

Events 0

Trigger	Assignments