Wee1Pc has been defined as boundarycondition, only change with the assignment rule.

Simulation of Figure1, total amounts of String are constant, 0.8.

Total Wee1 are constant 0.8.

kdstg=0 for figure1B

ksstg=0 for figure1B

 Wee1T N E_1 Wee1n Wee1Pn 1 N E_1 Wee1c 1 N E_1 StgPc N E_1 StgPn 1 N E_1 MPFc preMPFc N E_1 MPFn preMPFn 0 ksc cytoplasm nuclei kdmp Stgm Jm Stgm kdm Xp Stgm cytoplasm ksstg Stgm cytoplasm kastgp kastg MPFc Stgc Jastg Stgc cytoplasm kistg StgPc Jistg StgPc cytoplasm kdstg Stgc cytoplasm kdstg StgPc cytoplasm kt MPFc E_1 N 1 N E_1 cytoplasm kstgp kstg StgPc preMPFc cytoplasm kt preMPFc E_1 N 1 N E_1 nuclei koutw_1 Wee1Pn cytoplasm kinw_1 Wee1Pc E_1 N 1 N E_1 nuclei koutw_1 Wee1n cytoplasm kinw_1 Wee1c E_1 N 1 N E_1 nuclei kouts_1 StgPn cytoplasm kins_1 StgPc E_1 N 1 N E_1 nuclei kouts_1 Stgn cytoplasm kins_1 Stgc E_1 N 1 N E_1 nuclei kstgp kstg StgPn preMPFn cytoplasm kweep kwee Wee1c MPFc nuclei kweep kwee Wee1n MPFn nuclei kdnp kdn FZYa preMPFn nuclei kdnp kdn FZYa MPFn nuclei kiie IEa_1 Jiie IEa_1 nuclei kaie 1 IEa_1 MPFn Jaie 1 IEa_1 nuclei kafzy IEa_1 1 FZYa Jafzy 1 FZYa nuclei kifzy FZYa Jifzy FZYa nuclei kiweep kiwee MPFn Wee1n Jiwee Wee1n nuclei kawee Wee1Pn Jawee Wee1Pn nuclei kastgp kastg MPFn Stgn Jastg Stgn nuclei kistg StgPn Jistg StgPn nuclei kdstg Stgn nuclei kdstg StgPn cytoplasm kdc preMPFc cytoplasm kdc MPFc cytoplasm kiweep kiwee MPFc Wee1c Jiwee Wee1c cytoplasm kawee Wee1Pc Jawee Wee1Pc cytoplasm kt MPFc cytoplasm kt preMPFc nuclei koutw_1 Wee1Pn N E_1 1 N E_1 cytoplasm kinw_1 Wee1Pc nuclei koutw_1 Wee1n N E_1 1 N E_1 cytoplasm kinw_1 Wee1c nuclei kouts_1 StgPn E_1 N 1 N E_1 cytoplasm kins_1 StgPc nuclei kouts_1 Stgn E_1 N 1 N E_1 cytoplasm kins_1 Stgc nuclei ksxm_1 N cytoplasm ksxp_1 Xm nuclei kout_1 MPFn nuclei kout_1 preMPFn nuclei kout_1 MPFn E_1 N 1 N E_1 nuclei kout_1 preMPFn N E_1 1 N E_1

When Fzy=Kez(increasing), then the following changes are made instantaneously (for X=MPF,preMPF,Wee1,Wee1P, Stg, and StgP) :

Xc-> (1-N*E)*Xc/(1-1.95*N*E)

Xn->Xn/1.95

N->1.95N

 FZYa kez_1 MPFc 1 N E_1 1 1.95 N E_1 StgPn 1.95 Stgc 1 N E_1 1 1.95 N E_1 StgPc 1 N E_1 1 1.95 N E_1 Stgn 1.95 Wee1c 1 N E_1 1 1.95 N E_1 Wee1Pn 1.95 Wee1n 1.95 preMPFn 1.95 MPFn 1.95 factor_1 N